scholarly journals Biochemical Characterization of Eight Genetic Variants of Human DNA Polymerase κ Involved in Error-Free Bypass across Bulky N2-Guanyl DNA Adducts

2014 ◽  
Vol 27 (5) ◽  
pp. 919-930 ◽  
Author(s):  
Insil Song ◽  
Eun-Jin Kim ◽  
In-Hyeok Kim ◽  
Eun-Mi Park ◽  
Kyung Eun Lee ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Adducts ◽  
Genetic Variants ◽  
Biochemical Characterization ◽  
Human Dna ◽  
Bulky N2

scholarly journals Characterization of human DNA polymerase delta and its immunochemical relationships with DNA polymerase alpha and epsilon

1991 ◽  
Vol 266 (4) ◽  
pp. 2423-2429
Author(s):  
M Y Lee ◽  
Y Q Jiang ◽  
S J Zhang ◽  
N L Toomey
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase Delta ◽  
Dna Polymerase Alpha ◽  
Human Dna

Detection and characterization of DNA polymerase activity in Toxoplasma gondii

Parasitology ◽  
1993 ◽  
Vol 107 (2) ◽  
pp. 135-139 ◽  
Author(s):  
A. Makioka ◽  
B. Stavros ◽  
J. T. Ellis ◽  
A. M. Johnson
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Sedimentation Coefficient ◽  
Polymerase Activity ◽  
Magnesium Ions ◽  
Dna Polymerase Β ◽  
Human Dna ◽  
Approximate Molecular Weight

SUMMARYA DNA polymerase activity has been detected and characterized in crude extracts from tachzoites of Toxoplasma gondii. The enzyme has a sedimentation coefficient of 6·4 S, corresponding to an approximate molecular weight of 150000 assuming a globular shape. Like mammalian DNA polymerase α, the DNA polymerase of T. gondii was sensitive to N-ethylmaleimide and inhibited by high ionic strength. However, the enzyme activity was not inhibited by aphidicolin which is an inhibitor of mammalian DNA polymerases α, δ and ε and also cytosine-β-D-arabinofuranoside-5′-triphosphate which is an inhibitor of α polymerase. The activity was inhibited by 2′,3′-dideoxythymidine-5′-triphosphate which is an inhibitor of mammalian DNA polymerase β and γ. Magnesium ions (Mg2+) were absolutely required for activity and its optimal concentration was 6 mM. The optimum potassium (K+) concentration was 50 mM and a higher concentration of K+ markedly inhibited the activity. Activity was optimal at pH 8. Monoclonal antibodies against human DNA polymerase did not bind to DNA polymerase of T. gondii. Thus the T. gondii enzyme differs from the human enzymes and may be a useful target for the design of toxoplasmacidal drugs.

scholarly journals Characterization of a Natural Mutator Variant of Human DNA Polymerase λ which Promotes Chromosomal Instability by Compromising NHEJ

PLoS ONE ◽  
2009 ◽  
Vol 4 (10) ◽  
pp. e7290 ◽  
Author(s):  
Gloria Terrados ◽  
Jean-Pascal Capp ◽  
Yvan Canitrot ◽  
Miguel García-Díaz ◽  
Katarzyna Bebenek ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Chromosomal Instability ◽  
Human Dna ◽  
Dna Polymerase Λ

Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form

2015 ◽  
Vol 114 (4) ◽  
pp. 1313-1326 ◽  
Author(s):  
Edio Maldonado ◽  
Diego A. Rojas ◽  
Sandra Moreira-Ramos ◽  
Fabiola Urbina ◽  
Vicente J. Miralles ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Dna Polymerase ◽  
Biochemical Characterization ◽  
Recombinant Dna ◽  
Dna Polymerase Beta ◽  
Native Form ◽  
Polymerase Beta

scholarly journals The Effect of DNA Structure on the Catalytic Efficiency and Fidelity of Human DNA Polymerase β on Templates with Platinum-DNA Adducts

2001 ◽  
Vol 276 (22) ◽  
pp. 18999-19005 ◽  
Author(s):  
Alexandra Vaisman ◽  
Matthew W. Warren ◽  
Stephen G. Chaney
Keyword(s):  
Dna Polymerase ◽  
Dna Adducts ◽  
Catalytic Efficiency ◽  
Dna Structure ◽  
Dna Polymerase Β ◽  
Human Dna

Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme

2002 ◽  
Vol 7 (7) ◽  
pp. 639-651 ◽  
Author(s):  
Noriko Shimazaki ◽  
Kenta Yoshida ◽  
Toshiko Kobayashi ◽  
Shingo Toji ◽  
Katsuyuki Tamai ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Recombinant Enzyme ◽  
Over Expression ◽  
E Coli ◽  
Human Dna

Characterization of human DNA polymerase κ promoter in response to benzo[a]pyrene diol epoxide

2012 ◽  
Vol 33 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Huifang Zhu ◽  
Yanfeng Fan ◽  
Jing Shen ◽  
Hongyan Qi ◽  
Jimin Shao
Keyword(s):  
Dna Polymerase ◽  
Human Dna ◽  
Diol Epoxide

scholarly journals Purification and Characterization of the Catalytic Subunit of Human DNA Polymerase δ Expressed in Baculovirus-infected Insect Cells

1996 ◽  
Vol 271 (47) ◽  
pp. 29740-29745 ◽  
Author(s):  
Jin-Qiu Zhou ◽  
Cheng-Keat Tan ◽  
Antero G. So ◽  
Kathleen M. Downey
Keyword(s):  
Dna Polymerase ◽  
Insect Cells ◽  
Catalytic Subunit ◽  
Purification And Characterization ◽  
Dna Polymerase Δ ◽  
Human Dna ◽  
Infected Insect
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