scholarly journals A temporary immersion system improves in vitro regeneration of peach palm through secondary somatic embryogenesis

2011 ◽  
Vol 108 (8) ◽  
pp. 1463-1475 ◽  
Author(s):  
D. A. Steinmacher ◽  
M. P. Guerra ◽  
K. Saare-Surminski ◽  
R. Lieberei
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Regeneration ◽  
Secondary Somatic Embryogenesis ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
Peach Palm

scholarly journals In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 177 ◽  
Author(s):  
Valeria Gianguzzi ◽  
Paolo Inglese ◽  
Ettore Barone ◽  
Francesco Sottile
Keyword(s):  
Liquid Culture ◽  
Solid Medium ◽  
In Vitro Regeneration ◽  
Production Costs ◽  
Gelling Agent ◽  
Temporary Immersion System ◽  
Relative Growth ◽  
Temporary Immersion ◽  
Capparis Spinosa

Three caper (Capparis spinosa L.) biotypes grown on the Sicilian island of Salina (38°33′49″ N) were micropropagated to evaluate two different in vitro culture systems: one using the traditional solid medium, and the other based on liquid culture in a PlantForm bioreactor. PlantForm is a temporary immersion system (TIS), a new propagation method in which the shoots undergo temporary immersion in a liquid medium in order to avoid the accumulation of gas through forced ventilation. This study proposes a protocol to improve the efficiency of in vitro propagation of caper plants, while also reducing production costs, because of the elimination of the gelling agent, and manual labor, requiring limited subcultures and posing minimal contamination risks. Our results show that the caper shoots propagated in bioreactors demonstrated good adaptability and better growth rates than those grown in the conventional system. Statistically significant differences were observed between plants grown in the PlantForm liquid culture and those grown in solid medium regarding the number and length of shoots, which were further promoted by the addition of plant growth regulators (PGRs). The relative growth and real proliferation rate of the caper explants were higher when using meta-Topolin than when using 6-benzylaminopurine as a PGR. Overall, the TIS improved in vitro caper culture by promoting the proliferation, length, and vigor of the shoots.

Improved high-efficiency protocol for somatic embryogenesis in Peach Palm (Bactris gasipaes Kunth) using RITA® temporary immersion system

2014 ◽  
Vol 179 ◽  
pp. 284-292 ◽  
Author(s):  
Angelo S. Heringer ◽  
Douglas A. Steinmacher ◽  
Hugo P.F. Fraga ◽  
Leila N. Vieira ◽  
Tiago Montagna ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
High Efficiency ◽  
Bactris Gasipaes ◽  
Temporary Immersion System ◽  
Temporary Immersion ◽  
Peach Palm

Antimicrobial and hormetic effects of silver nanoparticles on in vitro regeneration of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system

2017 ◽  
Vol 129 (2) ◽  
pp. 195-207 ◽  
Author(s):  
J. L. Spinoso-Castillo ◽  
R. A. Chavez-Santoscoy ◽  
Nina Bogdanchikova ◽  
J. A. Pérez-Sato ◽  
V. Morales-Ramos ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
In Vitro Regeneration ◽  
Vanilla Planifolia ◽  
Temporary Immersion System ◽  
Temporary Immersion

scholarly journals In Vitro Culture of Rosmarinus officinalis L. in a Temporary Immersion System: Influence of Two Phytohormones on Plant Growth and Carnosol Production

2021 ◽  
Vol 14 (8) ◽  
pp. 747
Author(s):  
Eder Villegas Sánchez ◽  
Mariana Macías-Alonso ◽  
Soraya Osegueda Robles ◽  
Lisset Herrera-Isidrón ◽  
Hector Nuñez-Palenius ◽  
...  
Keyword(s):  
High Performance ◽  
Emerging Infectious Diseases ◽  
Rosmarinus Officinalis ◽  
Wild Plant ◽  
Array Detector ◽  
Phase System ◽  
Naphthaleneacetic Acid ◽  
Temporary Immersion System ◽  
Temporary Immersion

Emerging infectious diseases have become a major global problem with public health and economic consequences. It is an urgent need to develop new anti-infective therapies. The natural diterpene carnosol exhibit a wide variety of interesting antibacterial and antiviral properties, and it is considered a theoretical inhibitor of COVID-19 Mpro. However, this compound is present in the family Lamiaceae in low quantities. To obtain carnosol in concentrations high enough to develop pharmacological studies, we evaluated the efficiency of a micropropagation protocol of Rosmarinus officinalis using a solid medium and a temporary immersion system (TIS), as well as the effect of 6-benzylaminopurine (6-BAP) and α-naphthaleneacetic acid (NAA) on the growth of shoots. Moreover, we developed and validated an analytical method to quantify carnosol using the H-point standard additions method in the high-performance liquid chromatography diode array detector (HPLC-DAD). After 30 days of culture, TIS produced the maximum number of shoots per explant (24.33 ± 1.15) on a liquid medium supplemented with 6-BAP at 5.0 mg L−1. Next, we also evaluated the effect of immersion time and frequency for TIS. After 72 days of culture, the best results were obtained with an immersion cycle of 1 min every 12 h, yielding 170.33 ± 29.40 shoots. The quantification of carnosol on the samples was performed at a flow rate of 1.2 mL min−1 using binary isocratic mobile phase system 60:40 (v/v) 10 mM formic acid (pH 3.0) (A) and acetonitrile (B) on a reverse-phase column. The content of carnosol in the in vitro cultures was around 8-fold higher than in the wild plant. The present study represents an efficient alternative method to obtain carnosol for its pre-clinical and clinical development.

scholarly journals In vitro Regeneration of Dryland Kenyan Maize Genotypes Through Somatic Embryogenesis

2006 ◽  
Vol 2 (2) ◽  
pp. 146-151 ◽  
Author(s):  
R.O. Oduor ◽  
E.N.M. Njagi ◽  
S. Ndung` u ◽  
J.S. Machuka
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Regeneration ◽  
Maize Genotypes

AN ALTERNATIVE PATHWAY FOR PLANT IN VITRO REGENERATION OF INSECTICIDAL TREE MELIA AZEDARACH L. DERIVED FROM THE INDUCTION OF SOMATIC EMBRYOGENESIS

2006 ◽  
pp. 489-494
Author(s):  
S. Sharry ◽  
W. Abedini ◽  
S. Lede ◽  
J.L. Cabrera Ponce ◽  
L. Herrera Estrella ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Regeneration ◽  
Alternative Pathway ◽  
Melia Azedarach

In vitro Regeneration of Irvingia gabonensis by Somatic Embryogenesis

2008 ◽  
Vol 11 (5) ◽  
pp. 726-732 ◽  
Author(s):  
Fotso . ◽  
Oumar . ◽  
Niemenak Nicolas ◽  
Donfagsiteli Tchinda Ne ◽  
Omokolo Ndoumou De
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Regeneration ◽  
Irvingia Gabonensis

scholarly journals ESTABLECIMIENTO DEL SISTEMA DE REGENERACION POR EMBRIOGÉNESIS SOMÁTICA DE Azadirachta indica A. Juss.

2014 ◽  
Vol 20 (2) ◽  
Author(s):  
Maria D. Artigas R ◽  
Rafael Fernandez Da Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Azadirachta Indica ◽  
Culture System ◽  
Plant Metabolites ◽  
Secondary Somatic Embryogenesis ◽  
Short Term ◽  
Efficient System ◽  
Low Concentrations ◽  
Whole Plants

<p><em>Azadirachta indica</em>, es una planta con múltiples aplicaciones tanto forestal como farmacológica. Por ende, el establecimiento del sistema de cultivo <em>in vitro</em> por embriogénesis somática ofrece diversas y variadas ventajas, tales como obtener plantas altamente productivas en metabolitos. En este estudio, se utilizaron secciones foliares y cotiledonares, inducidas en medios MS (1962) suplementados con: BAP sólo y combinado con ANA/2,4-D, TDZ sólo y con ABA. La regeneración fue con MS sólo o con K + AIA y BAP + AIA. Como resultado se estableció un sistema eficiente con secciones de cotiledones, observándose organogénesis a bajas concentraciones de BAP, mientras a altos niveles de BAP (2,5 mg.L<sup>-1</sup>), así como  con TDZ + ABA (0,02 + 1mg.L<sup>-1</sup>) respectivamente favorecieron la embriogénesis somática primaria y secundaria en un 96 % y 71 % respectivamente. La regeneración fue 71 % con MS, mientras que el enraizamiento fue de 86,67 % con MS½, obteniéndose plantas completas a corto plazo. </p><p><strong>ABSTRACT</strong></p><p><em>Azadirachta indica</em>, is a plant with multiple forest and pharmacological application. Therefore, the establishment of <em>in vitro</em> culture system for somatic embryogenesis offers several distinct advantages such as obtaining highly productive plant metabolites. In this study, were used sections cotyledon and leaf, induced on MS medium (1962) supplemented with: BAP alone and combined with NAA / 2,4-D, TDZ alone and ABA. Regeneration was with MS alone or with K + BAP + IAA and IAA. As a result was established an efficient system with cotyledon sections, being observed organogenesis at low concentrations of BAP, while high levels of BAP (2.5 mg.L<sup>-1</sup>) with 96 % and TDZ + ABA (0.02 + 1mg.L<sup>-1</sup>) with 71 %, favoring the primary and secondary somatic embryogenesis. Regeneration was 71 % with MS; rooting was 86.67 % with MS½, presenting whole plants obtained short term.</p><p><strong>RESUMO</strong></p><p><em>Azadirachta indica, </em>é uma planta com várias aplicaçãos florestas e farmacológicas. Por conseguinte, a criação de sistema de cultur<em>a in vitro </em>para a embriogénese somática apresenta várias vantagens, tais como a obtenção de plantas altamente produtivas de metabolitos. Neste estudo, as seções de cotilédones e folhas, induzidas em meios suplementado en MS (1962) con: BAP sozinho e combinado com ANA / 2,4-D, TDZ e ABA sozinho. A regeneração foi apenas com MS ou com K + BAP + IAA e IAA. Como resultado, foi eficiente con seções cotiledones, observados organogênese em baixas concentrações de BAP, en quanto altos níveis de BAP (2,5 mg L-1) e com TDZ + ABA (0,02 + 1mg.L-1) favoreceu a embriogênese somática primária e secundária até 96 % e 71%, respectivamente. Regeneração estava com MS 71 %, en quanto que o enraizamento foi de 86,67 %, com ½ MS produzindo plantas inteiras curto prazo.</p><p><strong><br /></strong></p><p> </p>

scholarly journals In vitro regeneration and somatic embryogenesis in citrus

2015 ◽  
Vol 24 (2) ◽  
pp. 247-262 ◽  
Author(s):  
El Sawy A Mohamed ◽  
Amina Gomaa ◽  
Nancy Danial
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Rt Pcr ◽  
Mother Plants

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December

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