Comparison of immunocytochemistry, reverse transcriptase polymerase chain reaction, and nucleic acid sequence–based amplification for the detection of circulating breast cancer cells

1999 ◽  
Vol 56 (3) ◽  
pp. 217-229 ◽  
Author(s):  
A.C. Lambrechts ◽  
A.J. Bosma ◽  
S.G. Klaver ◽  
B. Top ◽  
L. Perebolte ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Reverse Transcriptase ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Nucleic Acid Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

Evaluation of quantitative polymerase chain reaction markers for the detection of breast cancer cells in ovarian tissue stored for fertility preservation

2015 ◽  
Vol 104 (2) ◽  
pp. 410-417.e4 ◽  
Author(s):  
Laurence Bockstaele ◽  
Selma Boulenouar ◽  
Géraldine Van Den Steen ◽  
Julie Dechène ◽  
Sophie Tsepelidis ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Cancer Cells ◽  
Fertility Preservation ◽  
Breast Cancer Cells ◽  
Ovarian Tissue ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells by suppressing the PI3K/Akt/mTOR signalling pathway

2020 ◽  
Author(s):  
Akosua Kotaa Kwakye ◽  
Sylvanus Kampo ◽  
Jiaxin Lv ◽  
Muhammad Noman Ramzan ◽  
Seidu A. Richard ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Western Blot ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Mtor Signalling ◽  
Mtor Signalling Pathway

Abstract Objective This study aimed to test the hypothesis that levobupivacaine has anti-tumour effects on breast cancer cells Results Colony formation and transwell assay were used to determine breast cancer cells proliferation. Flow Cytometry (annexin V and PI staining) was used to investigate breast cancer cells apoptosis. The effects of levobupivacaine on cellular signalling and molecular response were studied with Quantitative Polymerase Chain Reaction and western blot. Induction of apoptosis was confirmed by cell viability, morphological changes showed cell shrinkage, rounding, and detachments from plates. The results of the western blot and Quantitative Polymerase Chain Reaction indicated activation of active caspase-3 and inhibition of FOXO1. The results of the flow Cytometry confirmed that levobupivacaine inhibited breast cancer cell proliferation and enhanced apoptosis of breast cancer cells. Quantitative Polymerase Chain Reaction and Western blot analysis showed increased p21 and decreased cyclin D. Quantitative Polymerase Chain Reaction and western blot analysis showed that levobupivacaine significantly increased Bax expression, accompanied by a significant decreased Bcl-2 expression and inhibition of PI3K/Akt/mTOR signalling pathway. These findings suggested that levobupivacaine inhibits proliferation and promotes breast cancer cells apoptosis in vitro.

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