Plant Regeneration from Immature Embryo Cultures of Vigna unguiculata

2003 ◽  
Vol 46 (2) ◽  
pp. 305-308 ◽  
Author(s):  
P.-S. Choi ◽  
D.-Y. Cho ◽  
W.-Y. Soh
Keyword(s):  
Plant Regeneration ◽  
Vigna Unguiculata ◽  
Immature Embryo ◽  
Embryo Cultures

Regeneration of immature and mature embryos from diverse sets of wheat genotypes using media containing different auxins

2012 ◽  
Vol 60 (2) ◽  
pp. 97-108 ◽  
Author(s):  
R. Murín ◽  
K. Mészáros ◽  
P. Nemeček ◽  
R. Kuna ◽  
J. Faragó
Keyword(s):  
Plant Regeneration ◽  
Immature Embryo ◽  
Shoot Formation ◽  
Mature Embryo ◽  
Regeneration Ability ◽  
Wheat Embryo ◽  
Mature Embryos ◽  
Embryo Cultures ◽  
2,4 Dichlorophenoxyacetic Acid

The effect of explant type (immature vs. mature embryos) and two auxin types (2,4-dichlorophenoxyacetic acid vs. Dicamba) on the callogenesis and plant regeneration ability of 26 wheat cultivars was studied. In general, the callus induction, plant regeneration and shoot formation frequencies were higher in mature embryo-derived cultures as compared to immature ones on media originally developed for mature wheat embryo cultures. In both culture types, the auxin Dicamba was found to be more efficient, especially when mature embryos were cultured. The separation of means using Duncan’s multiple range test revealed the best in vitro response, in terms of the frequency of callus regeneration, in the cultivar Astella for both immature and mature embryo cultures. This cultivar gave very promising results, suggesting that it could be used in the future for further tissue culture investigations and as a donor material for genetic transformation experiments in wheat. Correlation analyses revealed significant similarities between the evaluated parameters within each group (immature and mature embryo-derived cultures). However, there were no significant correlations between these two groups for most of the parameters. This suggests that the mechanism of plant regeneration in the two in vitro regeneration systems (mature vs. immature embryo culture) may be different enough to hamper the development of an optimal plant regeneration protocol for use in both systems.

Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes

2009 ◽  
Vol 56 (4) ◽  
pp. 540-545 ◽  
Author(s):  
N. Mitić ◽  
D. Dodig ◽  
R. Nikolić ◽  
S. Ninković ◽  
D. Vinterhalter ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Immature Embryo ◽  
Donor Plant ◽  
Wheat Genotypes ◽  
Embryo Cultures

Optimisation of Tissue Culture Conditions for Transformation Studies Using Immature Embryos of Australian Barley Cultivars

1995 ◽  
Vol 43 (5) ◽  
pp. 499 ◽  
Author(s):  
AM Walmsley ◽  
RJ Henry ◽  
RG Birch
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Immature Embryo ◽  
Culture Conditions ◽  
Medium Composition ◽  
Immature Embryos ◽  
Systematic Testing ◽  
Barley Cultivars ◽  
Key Variables

Eight Australian barley cultivars were tested for efficiency of embryonic callus initiation and plant regeneration, from immature embryo explants in tissue culture. Optimisation of tissue culture conditions was performed for cultivars Bandulla, Clipper, Schooner and Tallon in an attempt to increase regeneration frequencies to levels suitable for genetic engineering of barley. Variables tested were 2,4-D concentration, salt composition, carbon source and immature embryo explant. Optimal culture medium composition varied between cultivars. Shoot regeneration rates from culture of isolated scutellar tissues were low for all four cultivars. Halved, immature embryos produced most shoots for cultivars Clipper, Schooner and Tallon, whereas Bandulla performed best with entire immature embryo explants. Clipper (a malting barley) and Bandulla (a feed barley) are suggested as model Australian cultivars for transformation studies. Immature embryos of Bandulla produced an average of 5.3 shoots and Clipper 10.1 shoots per embryo under optimal conditions. Our results show that rates of somatic embryo and plant regeneration sufficient for use in transformation studies can be achieved for diverse Australian Barley cultivars, through systematic testing of a range of key variables including explant type and medium composition.

An efficient method for immature embryo rescue and plant regeneration from the Calli ofZiziphus jujuba‘Lengbaiyu’

2018 ◽  
Vol 94 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Haiyan Ren ◽  
Xuemei Du ◽  
Dengke Li ◽  
Ailing Zhao ◽  
Yongkang Wang ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Efficient Method ◽  
Embryo Rescue ◽  
Immature Embryo
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