Estimation of Genetic Variability of Vigna radiata Cultivars by RAPD Analysis

2004 ◽  
Vol 48 (2) ◽  
pp. 205-209 ◽  
Author(s):  
S. Betal ◽  
P. Chowdhury ◽  
S. Kundu ◽  
S. Raychaudhuri
Keyword(s):  
Genetic Variability ◽  
Vigna Radiata ◽  
Rapd Analysis

Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 201-210 ◽  
Author(s):  
F. N. Wachira ◽  
R. Waugh ◽  
W. Powell ◽  
C. A. Hackett
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Southeast Asia ◽  
Camellia Sinensis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Systematic Assessment ◽  
Tree Crop ◽  
Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

scholarly journals Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

2016 ◽  
Vol 97 (6) ◽  
pp. 1307-1315 ◽  
Author(s):  
Elangovan Dilipan ◽  
Jutta Papenbrock ◽  
Thirunavakkarasu Thangaradjou
Keyword(s):  
Genetic Variability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Seagrass Species ◽  
Complex Species ◽  
Marine Angiosperms ◽  
Different Populations ◽  
High Degree ◽  
Identification Tool

In India 14 seagrass species can be found with monospecific genera (Enhalus, ThalassiaandSyringodium),Cymodoceawith two species andHalophilaandHalodulerepresented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of theHalophilaandHalodulecomplex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.

scholarly journals The use of random amplified polymorphic DNA to evaluate the genetic variability of Ponkan mandarin (Citrus reticulata Blanco) accessions

2000 ◽  
Vol 23 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Helvécio Della Coletta Filho ◽  
Marcos Antonio Machado ◽  
M. Luiza P.N. Targon ◽  
Jorgino Pompeu Jr.
Keyword(s):  
Genetic Variability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Point Mutations ◽  
The Other ◽  
Citrus Reticulata ◽  
Random Primers ◽  
Citrus Reticulata Blanco ◽  
Ponkan Mandarin

RAPD analysis of 19 Ponkan mandarin accessions was performed using 25 random primers. Of 112 amplification products selected, only 32 were polymorphic across five accessions. The absence of genetic variability among the other 14 accessions suggested that they were either clonal propagations with different local names, or that they had undetectable genetic variability, such as point mutations which cannot be detected by RAPD.

scholarly journals Genetic variability in segregating generations of greengram (Vigna radiata L.Wilczek) for quantitative traits.

2019 ◽  
Vol 10 (1) ◽  
pp. 293
Author(s):  
I. Mariyammal ◽  
M. Pandiyan ◽  
C. Vanniarajan ◽  
J. S. Kennedy ◽  
N. Senthil
Keyword(s):  
Genetic Variability ◽  
Vigna Radiata ◽  
Quantitative Traits

scholarly journals The RAPD Analysis of Genetic Variability in the Granary Weevil (Sitophilus Granarius L.) Populations

2008 ◽  
Vol 48 (4) ◽  
Author(s):  
Katarzyna Nowaczyk ◽  
Aleksandra Obrępalska-Stęplowska ◽  
Magdalena Gawlak ◽  
Paweł Olejarski ◽  
Jan Nawrot
Keyword(s):  
Genetic Variability ◽  
Rapd Analysis ◽  
Sitophilus Granarius ◽  
Granary Weevil

scholarly journals Occurrence and genetic variability of Candida parapsilosis sensu lato in Hungary

2007 ◽  
Vol 56 (2) ◽  
pp. 190-195 ◽  
Author(s):  
Sándor Kocsubé ◽  
Mónika Tóth ◽  
Csaba Vágvölgyi ◽  
Ilona Dóczi ◽  
Miklós Pesti ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Candida Parapsilosis ◽  
Sequence Data ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Its Region ◽  
Molecular Data ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Group I

The occurrence and genetic variability of Candida parapsilosis isolates in two Hungarian hospitals, located in Debrecen and Pécs, were examined. Among the 209 Candida isolates examined, 20 were found to belong to C. parapsilosis sensu lato, based on morphological, physiological and molecular data. The frequency of occurrence of C. parapsilosis isolates (9.6 %) was lower than that observed in Europe but higher than that observed previously in Hungary. The genetic variability of C. parapsilosis sensu lato isolates was also examined using random amplified polymorphic DNA (RAPD) analysis and sequence analysis of the intergenic transcribed spacer (ITS) region of the rRNA gene cluster. The genetic variability of the isolates was relatively high, as revealed by RAPD analysis. Two isolates were found to belong to the recently described Candida metapsilosis species (C. parapsilosis group III), based on ITS sequence data, RAPD analysis and phenotypic data. These two isolates could also be distinguished from C. parapsilosis sensu stricto isolates using a primer pair developed for the detection of C. parapsilosis group I isolates. To the best of the authors' knowledge, this is the first report on the identification of C. metapsilosis from bloodstream infection.

A Study on the Genetic Variability of Biatora Helvola using Rapd Markers

1999 ◽  
Vol 31 (5) ◽  
pp. 491-499 ◽  
Author(s):  
Christian Printzen ◽  
H. Thorsten Lumbsch ◽  
Imke Schmitt ◽  
G. Benno Feige
Keyword(s):  
Genetic Variability ◽  
Central Europe ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genetic Differences ◽  
Disjunct Distribution ◽  
Spruce Forest ◽  
Beech Forests ◽  
Last Glaciation ◽  
The Alps

AbstractBiatora helvola is a corticolous crustose lichen occurring in boreal and montane spruce and spruce-fir-beech forests. After the last glaciation, spruce reinvaded Europe from three refugia situated in the Carpathians, southeastern parts of the Alps and the Ural Mts., resulting in a slightly disjunct distribution. Our aim was to find out whether the glacial fragmentation of the distributional area of spruce is reflected by genetic differences in a typical spruce-forest lichen. Collections of Biatora helvola from Scandinavia and various parts of Central Europe were investigated using RAPD analysis. Algal free periclinal sections of the apothecia were obtained using a freezing microtome and transferred directly into PCR tubes. Six different RAPD primers were used. The data were analysed using PAUP*. It was shown that genetic differences between samples of B. helvola reflect the glacial disjunction of spruce in Europe.

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