Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing

2004 ◽  
Vol 14 (2) ◽  
pp. 185-197 ◽  
Author(s):  
Anastasia Missiou ◽  
Kriton Kalantidis ◽  
Alexandra Boutla ◽  
Sergia Tzortzakaki ◽  
Martin Tabler ◽  
...  
2018 ◽  
Author(s):  
Marta Grech-Baran ◽  
Kamil Witek ◽  
Katarzyna Szajko ◽  
Agnieszka I Witek ◽  
Karolina Morgiewicz ◽  
...  

ABSTRACTPotato virus Y (PVY) is a major potato pathogen that causes annual losses of billions of dollars. Control of its transmission requires extensive use of environmentally damaging insecticides. Rysto confers extreme resistance (ER) to PVY and is a valuable trait in resistance breeding programs. We isolated Rysto using Resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences Single-Molecule Real Time Sequencing). Rysto encodes a nucleotide binding-leucine rich repeat (NLR) protein with an N-terminal TIR domain, and is sufficient for PVY perception and extreme resistance in transgenic potato plants. We investigated the requirements for Rysto-dependent extreme resistance, and showed that Rysto function is temperature-independent and requires EDS1 and NRG1 proteins. Rysto may prove valuable for creating PVY-resistant cultivars of potato and other Solanaceae crops.


2002 ◽  
Vol 30 (2) ◽  
pp. 177-185 ◽  
Author(s):  
V. Doreste ◽  
P. L. Ramos ◽  
G. A. Enríquez ◽  
R. Rodríguez ◽  
R. Peral ◽  
...  

Planta ◽  
1999 ◽  
Vol 210 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Sophia Biemelt ◽  
Mohammad Reza Hajirezaei ◽  
Michael Melzer ◽  
Gerd Albrecht ◽  
Uwe Sonnewald

1999 ◽  
Vol 55 (3) ◽  
pp. 143-150 ◽  
Author(s):  
J. HINRICHS-BERGER ◽  
M. HARFOLD ◽  
S. BERGER ◽  
H. BUCHENAUER

Sign in / Sign up

Export Citation Format

Share Document