Plant Regeneration from Immature Zygotic Embryo-Derived Embryogenic Calluses and Cell Suspension Cultures of Catharanthus roseus

2004 ◽  
Vol 76 (2) ◽  
pp. 131-135 ◽  
Author(s):  
Suk Weon Kim ◽  
Dong Su In ◽  
Pil Son Choi ◽  
Jang R. Liu
1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.


2018 ◽  
Vol 13 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Eun Yee Jie ◽  
Nornita Sham Atong ◽  
Woo Seok Ahn ◽  
Myung Suk Ahn ◽  
Byung Hwan Min ◽  
...  

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 743-748
Author(s):  
Suvi Häkkinen ◽  
Heli Nygren ◽  
Natalia Maiorova ◽  
Raisa Haavikko ◽  
Sami Alakurtti ◽  
...  

AbstractIn this study, three semisynthetic betulonic acid-based compounds, 20(29)-dihydrolup-2-en[2,3-d]isoxazol-28-oic acid, 1-betulonoylpyrrolidine, and lupa-2,20(29)-dieno[2,3-b]pyrazin-28-oic acid, were studied in biotransformation experiments using Nicotiana tabacum and Catharanthus roseus cell suspension cultures. Biotransformation was performed using cyclodextrin to aid dissolving poorly water-soluble substrates. Several new derivatives were found, consisting of oxidized and glycosylated (pentose- and hexose-conjugated) products.


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