Reduction in Carbonic Anhydrase Activity in the Tongue Epithelium and Submandibular Gland in Zinc-Deficient Rats

We investigated the effects of zinc deficiency on carbonic anhydrase (CA) activity in the tongue epithelium and submandibular gland in rats. Male 4-week-old SD rats were given free access to a diet containing 2.2 (zinc-deficient), 4.1 (low-zinc), or 33.7 (zinc-sufficient) mg zinc/kg diet for 6 weeks. Rats in the fourth group (receiving 33.7 mg zinc/kg) were pair-fed against the zinc-deficient rats. Biochemical analysis at the end of the experimental period indicated that zinc deficiency significantly reduced CA activity in the tongue epithelium and submandibular gland, and the CA activity levels in these tissues seemed to parallel the dietary zinc levels. By enzyme histochemistry, an intensely positive reaction for CA was observed in the middle and basal regions of the taste buds in the circumvallate papilla in the zinc-sufficient and pair-fed (control) rats. The cells in von Ebner’s glands also showed a strong positive reaction in control rats, although only a weak reaction product was found in zinc-deficient rats. These results suggest that CA activity is affected by the dietary content of zinc, which is considered to be an indispensable factor for the maintenance of normal taste sensation.

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Effect of Reduction in Carbonic Anhydrase Activity Caused by Zinc Deficiency on CO2 Transfer Resistance

Photosynthesis: Mechanisms and Effects ◽

10.1007/978-94-011-3953-3_806 ◽

1998 ◽

pp. 3455-3458 ◽

Cited By ~ 1

Author(s):

Haruto Sasaki ◽

Tatsuro Hirose ◽

Yoshito Watanabe ◽

Ryu Ohsugi

Keyword(s):

Carbonic Anhydrase ◽

Zinc Deficiency ◽

Carbonic Anhydrase Activity ◽

Transfer Resistance

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Carbonic Anhydrase Activity as an Indicator of Zinc Deficiency in Citrus Leaves

Journal of Horticultural Science ◽

10.1080/00221589.1969.11514318 ◽

1969 ◽

Vol 44 (4) ◽

pp. 359-362 ◽

Cited By ~ 26

Author(s):

A. Bar-Akiva ◽

RUTH Lavon

Keyword(s):

Carbonic Anhydrase ◽

Zinc Deficiency ◽

Carbonic Anhydrase Activity ◽

Citrus Leaves

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Effects of zinc deficiency on developmental changes in alkaline phosphatase and carbonic anhydrase activities in the submandibular gland of the rat

Archives of Oral Biology ◽

10.1016/0003-9969(83)90009-2 ◽

1983 ◽

Vol 28 (7) ◽

pp. 609-615 ◽

Cited By ~ 9

Author(s):

D.W. Gandor ◽

D.J. Fanslow ◽

Julia Meyer

Keyword(s):

Alkaline Phosphatase ◽

Carbonic Anhydrase ◽

Submandibular Gland ◽

Zinc Deficiency ◽

Developmental Changes

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Carbonic anhydrase activity as an indicator of zinc deficiency in pecan leaves

Plant and Soil ◽

10.1007/bf02143619 ◽

1983 ◽

Vol 74 (2) ◽

pp. 287-289 ◽

Cited By ~ 8

Author(s):

I. Snir

Keyword(s):

Carbonic Anhydrase ◽

Zinc Deficiency ◽

Carbonic Anhydrase Activity

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Branchial and Antennal Gland Na+/K+- Dependent ATPase and Carbonic Anhydrase Activity During Salinity Acclimation of the Euryhaline Crayfish Pacifastacus Leniusculus

Journal of Experimental Biology ◽

10.1242/jeb.133.1.73 ◽

1987 ◽

Vol 133 (1) ◽

pp. 73-86 ◽

Cited By ~ 4

Author(s):

MICHÈLE G. WHEATLY ◽

RAYMOND P. HENRY

Keyword(s):

Carbonic Anhydrase ◽

Sea Water ◽

Marine Species ◽

Carbonic Anhydrase Activity ◽

Pacifastacus Leniusculus ◽

Activity Levels ◽

Salinity Acclimation ◽

Antennal Gland ◽

The Difference ◽

Uptake Mechanisms

Haemolymph and urine electrolyte status and branchial and antennal gland activities of Na+/K+-ATPase and carbonic anhydrase (CA) were determined in the crayfish Padfastacus leniusculus after 3 weeks acclimation in fresh water (FW) and 350 and 750mosmolkg−1 sea water (SW). In FW the crayfish maintained haemolymph osmolality around 370 mosmol kg−1 due to hyperionic regulation of the major electrolytes. Involved in this are ion uptake mechanisms situated on the gills, and mechanisms of ion reabsorption from the primary urinary filtrate in the antennal gland (AG). Both of these processes are associated with high activities of Na+/K+- ATPase and CA. The two enzymes are uniformly distributed on gill sets 2–7, unlike the situation in euryhaline marine species. Additionally, activity levels of both enzymes are extremely high in the AG and can be correlated with the ability to produce a hypo-osmotic urine. In comparison, enzyme activity is negligible in marine species which produce isosmotic urine. Crayfish continued to hyperosmoregulate in 350 mosmolkg−1 SW. High levels of Na+/K+-ATPase confirmed the presence of a component active in the uptake of major electrolytes in the gills and also in the AG, where ion reabsorption persisted. In 750 mosmolkg−1 SW crayfish became isosmotic. Since ATPase is regulated chiefly by deactivation/activation of pre-existing enzyme, overall activity was mostly unchanged. CA activity was significantly reduced in both 350 and 750 mosmol kg−1 SW and correlated with the transition from osmoregulation to osmoconformity, suggesting that it is regulated primarily by deinduction/induction of new enzyme. The difference in the mechanism of regulation exhibited by these two enzymes is believed to relate to their subcellular distribution.

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