Levels of endogenous indole-3-acetic acid and indole3-acetylaspartic acid during adventitious root formation in pea cuttings

1991 ◽  
Vol 82 (4) ◽  
pp. 599-605 ◽  
Author(s):  
Ann-Caroline Nordstrom ◽  
Lennart Eliasson
Keyword(s):  
Acetic Acid ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Indole 3 Acetic Acid

Silicon induces adventitious root formation in rice (Oryza sativa L.) under arsenate stress with the involvement of nitric oxide and indole-3-acetic acid

2020 ◽  
Author(s):  
Durgesh Kumar Tripathi ◽  
Padmaja Rai ◽  
Gea Guerriero ◽  
Shivesh Sharma ◽  
Francisco J Corpas ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Acetic Acid ◽  
Adventitious Root ◽  
Root Formation ◽  
Oryza Sativa L ◽  
Adventitious Root Formation ◽  
Growth Media ◽  
Simultaneous Application ◽  
Arsenate Stress ◽  
Indole 3 Acetic Acid

Abstract Arsenic (As) negatively affects plant development. Using rice as a model, this study evaluates how the application of silicon (10 µM Si) can favour the formation of adventitious roots under arsenate stress (50 µM As V) as a mechanism to mitigate its negative effects. Indeed, the simultaneous application of As V and Si up-regulated the expression of genes involved in nitric oxide (NO) metabolism (OsNOA1), cell cycle progression (G2-M, OsCDKA1), auxin (IAA, indole-3-acetic acid) biosynthesis (OsYUCCA1 and OsTAA1) and transport (OsPIN1, OsPIN5 and OsPIN10) and Si uptake (OsLsi1 and OsLsi2), which accompanied adventitious root formation. Furthermore, Si triggered the expression and activity of MDHAR and DHAR involved in ascorbate recycling. The treatment with L-NAME, an inhibitor of NO generation, significantly suppressed adventitious root formation, even in the presence of Si; however, supplying NO in the growth media rescued its effects. The data obtained suggest that both NO and IAA are essential for Si-mediated adventitious root formation under As V stress. Interestingly, TIBA (a polar auxin transport inhibitor) suppressed adventitious root formation, even in the presence of Si and SNP (an NO donor), suggesting that Si is involved in a mechanism whereby a cellular signal is triggered and requires NO formation first and, then, IAA.

Stimulation of adventitious rooting in sunflower (Helianthus annuus) by low pH: possible role of auxin

1993 ◽  
Vol 71 (12) ◽  
pp. 1645-1650 ◽  
Author(s):  
Jin-Hao Liu ◽  
Ilabanta Mukherjee ◽  
David M. Reid
Keyword(s):  
Acetic Acid ◽  
Helianthus Annuus ◽  
Shoot Apex ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Low Ph ◽  
Acid Transport ◽  
Acidic Conditions ◽  
Indole 3 Acetic Acid

Adventitious root formation by the hypocotyl cuttings of sunflower seedlings was greatly affected by the pH of buffered and unbuffered solutions bathing their basal portion. Exposure to low pH for 5 h after original root excision promoted root formation. Reduction of endogenous indole-3-acetic acid movement from the cotyledons and shoot apex was achieved by using N-1-naphthylphthalamic acid (an inhibitor of indole-3-acetic acid transport) and by removal of the cotyledons and shoot apex. Both the inhibitor and organ removal inhibited adventitious root formation, but acidic conditions could, to varying degrees, overcome this inhibition. Acidic conditions also increased the rate of [3H]indole-3-acetic acid uptake from the solutions around the hypocotyl bases and the rate of [3H]indole-3-acetic acid movement from cotyledons to the hypocotyl bases. Thus, acidic conditions may stimulate rooting by increasing the rate of basipetal indole-3-acetic acid transport to the zone of root initiation. These experiments show that in studies of the effects of various substances on rooting, the experimenter must be aware of these pH effects and take appropriate precautions. Key words: adventitious roots, auxin, indole-3-acetic acid, Helianthus annuus, pH.

scholarly journals Immunohistochemical Localization of Indole-3-Acetic Acid During Induction of Adventitious Root Formation from Cotyledon Explants of Walnut

2011 ◽  
Vol 136 (5) ◽  
pp. 315-319 ◽  
Author(s):  
Ningguang Dong ◽  
Qingmin Wang ◽  
Junpei Zhang ◽  
Dong Pei
Keyword(s):  
Acetic Acid ◽  
Adventitious Root ◽  
Root Formation ◽  
Juglans Regia ◽  
Adventitious Root Formation ◽  
Vascular Bundles ◽  
Root Induction ◽  
Vascular Differentiation ◽  
Cotyledon Explants ◽  
Indole 3 Acetic Acid

Cotyledon explants of walnut (Juglans regia) have been shown to generate adventitious roots on growth regulator-free medium. The spatial distribution of endogenous indole-3-acetic acid (IAA) and its dynamic changes during adventitious root formation were investigated using an in situ immunohistochemical approach. Before root induction, IAA signal was distributed throughout the freshly excised cotyledon explants. During provascular bundle differentiation, the IAA signal was mainly located in the provascular bundles. At the stage of annular meristematic zones formation, the IAA signal was mainly distributed in the meristematic zones and decreased in the vascular bundles and cotyledonous parenchyma. As primordia formed, the IAA signal became localized in the root primordia and gradually disappeared in the meristematic zones. In emerging roots, the IAA signal was mainly localized in the root cap and root meristem. These results suggest that accumulation of IAA in the provascular bundles may induce vascular differentiation and the increase in IAA through meristematic zones may be responsible for the adventitious root formation from walnut cotyledons. The direct evidence presented here indicates that IAA accumulated in the meristematic zones is not the sole signal needed to induce adventitious root.

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