Analysis of Phenolic Components and Related Biological Activities of 35 Apple (Malus pumila Mill.) Cultivars

Apple (Malus pumila Mill.) is a popular fruit with high economic values and various biological activities that are beneficial to human health. In this study, 35 apple cultivars were collected and were evaluated for their basic quality indexes, phenolic compositions, antioxidant activity, anti-tumour, and anti-diabetic activities. The compositions of phenolics were detected by using high-performance liquid chromatography (HPLC) and high-resolution mass spectroscopy (HRMS) assays. The antioxidant activities of peel and pulp extracts from 35 apple cultivars were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and ferric reducing antioxidant power (FRAP) assay. Results showed that the contents of phenolic acids and proanthocyanidins showed significant correlations with the antioxidant activities. Phenolic-rich extracts significantly inhibited HepG2 cell proliferation, with the inhibition activity varied significantly between cultivars. ‘Gold Delicious’ pulp extract, ‘Xiboliyabaidian’ peel and pulp extracts showed protective effects on H2O2-induced injury of human umbilical vein endothelial cells (HUVEC). ‘Red Fuji’ peel extract, ‘Xiboliyabaidian’ peel and pulp extracts, as well as ‘Gold Delicious’ peel extract, significantly increased glucose consumption of HepG2 cells, in a dose-dependent manner. This research may provide theoretical guidance for further nutritional investigation of the apple resources.

Control of Insects and Diseases with Intelligent Variable-rate Sprayers in Ornamental Nurseries1

Intelligent spray technology can reduce pesticide use and safeguard the environment; however, its ability to effectively control insects and disease must be validated before its adoption by growers. Comparative tests for two different laser-guided variable-rate intelligent sprayers and the same sprayers with conventional constant-rate mode were conducted to control pests at two ornamental nurseries in two growing seasons in Ohio. Crabapple [Malus ‘Sutyzam’ (Sugar Tyme®), M. sargentii], apple (Malus pumila), maple [Acer ×freemanii ‘Jeffersred' (Autumn Blaze®), A. rubrum ‘Franksred' (Red Sunset®) and A. rubrum], birch (Betula nigra and Betula populifolia ‘Whitespire'), London planetree (Platanus ×acerifolia ‘Bloodgood') and dogwood (Cornus florida) were used as the test plants. Intelligent spray technology reduced pesticide use by 56.1% and 51.8% on average at the two nurseries, respectively. Compared to conventional air-assisted sprayers, severity of scab on apple trees and powdery mildew in dogwood was reduced on intelligent spray-treated plants at one nursery, and there were equal or fewer leafhoppers in maple trees and aphids in birch trees when sprayed using intelligent spray technology at both nurseries. These results suggest that intelligent, variable-rate sprayers achieve equivalent or greater insect and disease control in ornamental tree nurseries compared to conventional, constant-rate sprayers. Index words: aphid, apple scab, environmental protection, leafhopper, precision spray, powdery mildew, sustainable. Species used in this study: apple (Malus pumila Mill), birches (Betula nigra L, Betula populifolia Marsh. ‘Whitespire'), crabapples [Malus ‘Sutyzam' (Sugar Tyme®), M. sargentii Rehder], dogwood (Cornus florida L.), maples [Acer ×freemanii E. Murray ‘Jeffersred' (Autumn Blaze®), A. rubrum L. ‘Franksred'(Red Sunset®) and A. rubrum L.], London planetree [Platanus ×acerifolia (Ait.) Willd. ‘Bloodgood'].

Effect of Chitosan and Fish Gelatin Coatings on Preventing the Deterioration and Preserving the Quality of Fresh-Cut Apples

The effect of fish gelatin and chitosan coatings on the physicochemical characteristics of fresh-cut apples (Malus pumila Mill.), stored at 5 °C and 22 °C, was investigated. Chitosan provided an effective control for microbial growth, maintained firmness during 4 days of storage at room temperature (22 °C), and 12 days at refrigerator (5 °C). The results indicated that chitosan coating caused a significant decrease (p < 0.05) in the L* value of cube color of cut apples. Fish gelatin–chitosan coatings mitigated the L* value and decrease in hue angle of the cut apple samples, at cold storage. Experimental results showed that fish gelatin–chitosan and chitosan coatings, can be used to mitigate the formation of vitamin C, due to respiration, microbial growth, and weight loss at cold storage. Fish gelatin–chitosan coating might be a better combination for maintaining appearance and extending shelf-life of cut apples, compared to only chitosan coatings.