Effects of fusicoccin and indole-3-acetic acid on the levels of ascorbic acid and dehydroascorbic acid in the apoplast during elongation of epicotyl segments of Vigna angularis

1996 ◽  
Vol 98 (4) ◽  
pp. 731-736 ◽  
Author(s):  
Umeo Takahama
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Dehydroascorbic Acid ◽  
Vigna Angularis ◽  
Indole 3 Acetic Acid

Assay of L( + )-Ascorbic Acid in Buttermilk by Densitometric Transmittance Measurement of the Dehydroascorbic Acid Osazone

1974 ◽  
Vol 57 (1) ◽  
pp. 65-69
Author(s):  
Paul R Beljaars ◽  
Wouter Van Steenbergen Horrocks ◽  
Theo M M Rondags
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Quantitative Analysis ◽  
Thin Layer ◽  
Coefficient Of Variation ◽  
Dehydroascorbic Acid ◽  
Tlc Plates ◽  
The Relationship ◽  
Layer Chromatography ◽  
Flying Spot

Abstract A study is presented for the quantitative analysis of ascorbic acid in buttermilk samples, using densitometric transmittance measurements. The procedure is based on oxidation of ascorbic acid to dehydroascorbic acid, followed by reaction with 2,4-dinitrophenylhydrazine to form the dinitrosazone. The osazone is separated from interfering substances in the sample by thin layer chromatography (TLC) on silica gel G plates developed with chloroform-ethyl acetate-acetic acid (60+35+5) . The TLC plates are scanned with a flying-spot densitometer. The relationship between integrated signal and concentration is linear for 0.08—1.00 fig ascorbic acid (coefficient of variation 3—4%). Deviations from Beer's law start to appear at levels higher than 1.00 fig ascorbic acid. A mean coefficient of variation of 3.5±1.1% (P = 95%) was established for standard spot measurements (3 spots averaged) on 15 chromatoplates. Recovery of ascorbic acid added to buttermik was 98% (coefficient of variation 7.2%). Results of this study are compared with those reported for spectrophotometric, titrimetric, and potentiometric procedures. The proposed method is less accurate because of interferences and the subsequent variables which arise as a result of taking transmittance measurements through an opaque medium.

Modification of RNA Metabolism in Barley by Gibberellic Acid, Indole-3-Acetic Acid and 1-Ascorbic Acid

1978 ◽  
Vol 86 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Thomas Mathew ◽  
Indravadan Chhelshanker ^Dave ◽  
Bhagwan Krishan Gaur
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Gibberellic Acid ◽  
Rna Metabolism ◽  
Indole 3 Acetic Acid

scholarly journals Analysis of Vitamin C (ascorbic acid) Contents in Various Fruits and Vegetables by UV-spectrophotometry

1970 ◽  
Vol 42 (4) ◽  
pp. 417-424 ◽  
Author(s):  
Md Mizanur Rahman ◽  
Mohammad Mizanur Rahman Khan ◽  
Mohammad Mazedul Hosain
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Vitamin C ◽  
Fruits And Vegetables ◽  
Dehydroascorbic Acid ◽  
Storage Period ◽  
Uv Spectrophotometry ◽  
Total Vitamin ◽  
Red Color ◽  
Color Complex

Total vitamin C (ascorbic acid + dehydroascorbic acid) has been determined by UVspectrophotometric method in various fruits and vegetables. In this method bromine water oxidizes ascorbic acid to dehydroascorbic acid in presence of acetic acid. After coupling with 2,4-dinitrophenyl hydrazine at 37&deg:C temperature for three hours, the solution is treated with 85% H2SO4 to produce a red color complex and the absorbance was spectrophotometrically measured at 521 nm. The content of vitamin C were 10 mg/100g to 80 mg/100g in fruits and 16 mg/100g to 42 mg/100g in vegetables. Aloss of 64% at -10°C and 76% at 5°C was observed after two months storage period of one leafy vegetable "Enhydra fluctuans" (Helencha shak). Key words: Vitamin C determination, Vegetables, Fruits, UV-spectrophotometer.

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