Recovery and characterization of amino acid analog-resistant carrot and tobacco suspension-cultured cells after cryostorage for over 10 years

1998 ◽  
Vol 103 (2) ◽  
pp. 257-263 ◽  
Author(s):  
Sami Kamal ◽  
Sam Ethington ◽  
Ximing Luo ◽  
Jack Widholm
Keyword(s):  
Amino Acid ◽  
Cultured Cells ◽  
Suspension Cultured Cells ◽  
Acid Analog ◽  
Amino Acid Analog

Selection and characterization of cultured carrot and tobacco cells resistant to lysine, methionine, and proline analogs

1976 ◽  
Vol 54 (13) ◽  
pp. 1523-1529 ◽  
Author(s):  
Jack M. Widholm
Keyword(s):  
Amino Acid ◽  
Free Amino ◽  
Low Frequency ◽  
Free Proline ◽  
Daucus Carota L ◽  
Acid Analog ◽  
Nicotiana Tabacum L ◽  
Resistant Lines ◽  
Amino Acid Analog

Amino-acid-analog-resistant lines were selected from mutagen-treated suspension-cultured tobacco (Nicotiana tabacum L.) and carrot (Daucus carota L.) cells. Tobacco cell lines resistant to the lysine analogs aminoethyl-L-cysteine and delta-hydroxylysine and carrot lines resistant to a methionine analog and a proline analog, ethionine and hydroxyproline, respectively, were selected. The resistant lines were 10 to more than 1000 times less sensitive to the analogs than the parent strains. The resistant cells were selected with a low frequency, were generally stable during growth in the absence of the selective agent, and accumulated the corresponding natural free amino acid. The lysine-analog-resistant tobacco lines had more than 10 times the normal levels of free lysine while the methionine-analog-resistant carrot line had similar increases in free methionine. The hydroxyproline-resistant carrot line contained from 15 to 30 times the normal free proline levels and was cross resistant to certain other proline analogs.

Generation of a Tryptophan Radical in High Quantum Yield from a Novel Amino Acid Analog Using Near-UV/Visible Light

1997 ◽  
Vol 119 (28) ◽  
pp. 6457-6460 ◽  
Author(s):  
Doug Burdi ◽  
Béatrice M. Aveline ◽  
Paul D. Wood ◽  
JoAnne Stubbe ◽  
Robert W. Redmond
Keyword(s):  
Amino Acid ◽  
Quantum Yield ◽  
Visible Light ◽  
High Quantum Yield ◽  
High Quantum ◽  
Acid Analog ◽  
Amino Acid Analog ◽  
Uv Visible

Characterization of transmembrane auxin transport in Arabidopsis suspension-cultured cells

2014 ◽  
Vol 171 (6) ◽  
pp. 429-437 ◽  
Author(s):  
Daniela Seifertová ◽  
Petr Skůpa ◽  
Jan Rychtář ◽  
Martina Laňková ◽  
Markéta Pařezová ◽  
...  
Keyword(s):  
Auxin Transport ◽  
Cultured Cells ◽  
Suspension Cultured Cells
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