In vivo and in vitro effect of α-amanitin on preimplantation mouse embryo RNA polymerase

Nature ◽  
1974 ◽  
Vol 248 (5450) ◽  
pp. 678-680 ◽  
Author(s):  
CAROL M. WARNER ◽  
LARRY R. VERSTEEGH
Keyword(s):  
Rna Polymerase ◽  
Mouse Embryo ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Vitro Effect

Comparative effects of essential and nonessential metals on preimplantation mouse embryo development in vitro

Toxicology ◽  
1997 ◽  
Vol 116 (1-3) ◽  
pp. 123-131 ◽  
Author(s):  
Lynn A. Hanna ◽  
Jeffrey M. Peters ◽  
Lynn M. Wiley ◽  
Michael S. Clegg ◽  
Carl L. Keen
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

scholarly journals Effects of peritoneal fluid on preimplantation mouse embryo development and apoptosis in vitro

2005 ◽  
Vol 11 (5) ◽  
pp. 615-619 ◽  
Author(s):  
Navid Esfandiari ◽  
Tommaso Falcone ◽  
Jeffrey M Goldberg ◽  
Ashok Agarwal ◽  
Rakesh K Sharma
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Peritoneal Fluid ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse

Recombinant human leukemia inhibitory factor enhances the development of preimplantation mouse embryo in vitro

1999 ◽  
Vol 71 (4) ◽  
pp. 722-725 ◽  
Author(s):  
Horng-Der Tsai ◽  
Chi-Chen Chang ◽  
Yao-Yuan Hsieh ◽  
Hui-Yu Lo ◽  
Li-Wei Hsu ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Leukemia Inhibitory Factor ◽  
Inhibitory Factor ◽  
Human Leukemia ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse

X-Ray Sensitivity of the Preimplantation Mouse Embryo in Vitro

1975 ◽  
Vol 62 (2) ◽  
pp. 276 ◽  
Author(s):  
L. S. Goldstein ◽  
Akiko I. Spindle ◽  
Roger A. Pedersen
Keyword(s):  
Mouse Embryo ◽  
X Ray ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse

340. ANALYSIS OF PROTEINS SECRETED BY THE PREIMPLANTATION MOUSE EMBRYO

2010 ◽  
Vol 22 (9) ◽  
pp. 140
Author(s):  
R. Gentles ◽  
C. O'Neill
Keyword(s):  
Western Blot ◽  
Mouse Embryo ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Reproductive Technologies ◽  
Stage Of Development ◽  
Cellular Expression ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse

Many embryos generated by assisted reproductive technologies do not have the capacity for full development. A non-destructive test that allows the most viable embryos to be identified would allow development of a range of strategies to improve these technologies. Analysis of the proteinaceous secretome of the preimplantation mouse embryo by electrospray mass spectrometry of tryptic digests of embryo-conditioned culture media identified 20 peptides.1 Lactate dehydrogenase beta (LDH) and protein disulfide isomerase (PDI) were consistently among the most abundant peptides identified. PDI has been found to be secreted and surface expressed in a number of other cell types suggesting a biologically significant role. LDH-beta is a marker of plasma membrane integrity and is widely used in medicine for assessment of cardiac health. The aim of this study was to assess the expression of these proteins in the early embryo to gain some understanding of the potential significance of their presence within the embryonic secretome. Zygotes (B6CBF2) were collected 20–22 h post hCG. They were cultured in groups of 10 embryos for 96 h in 10 mL of modHTF 30 µg BSA/mL media. Embryonic protein was extracted for western blot analysis or embryos subjected were to immunolocalization studies for PDI and LDH. Western blot analysis revealed presence of proteins of expected molecular mass. Staining for both proteins occurred at high levels at each stage of development throughout pre-implantation development. Staining was predominantly cytoplasmic and excluded from nuclei. Cultured blastocysts had less LDH than fresh but the level for PDI was similar for both. Staining of non-permeabilized embryos revealed extracellular staining of PDI. The results show high levels of cellular expression of two proteins reported to be released by the embryo in vitro. (1) Beardsley A, Li Y, O’Neill C (2010). Characterization of a diverse secretome generated by the mouse preimplantation embryo in vitro. Reproductive Biology and Endocrinology 8, 71.

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