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Author(s):  
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Franco Radaelli ◽  
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Vittorio Terruzzi
2017 ◽  
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Pradeep Kakkadasam Ramaswamy ◽  
Amit Yelsangikar ◽  
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1996 ◽  
Vol 24 (10) ◽  
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Author(s):  
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Ronald W. Hilwig ◽  
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Giancarlo Spinzi ◽  
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Stephen M. Cohen ◽  
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...  

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Over the past five years it has become evident that radiation damage provides the fundamental limit to the study of blomolecular structure by electron microscopy. In some special cases structural determinations at very low doses can be achieved through superposition techniques to study periodic (Unwin & Henderson, 1975) and nonperiodic (Saxton & Frank, 1977) specimens. In addition, protection methods such as glucose embedding (Unwin & Henderson, 1975) and maintenance of specimen hydration at low temperatures (Taylor & Glaeser, 1976) have also shown promise. Despite these successes, the basic nature of radiation damage in the electron microscope is far from clear. In general we cannot predict exactly how different structures will behave during electron Irradiation at high dose rates. Moreover, with the rapid rise of analytical electron microscopy over the last few years, nvicroscopists are becoming concerned with questions of compositional as well as structural integrity. It is important to measure changes in elemental composition arising from atom migration in or loss from the specimen as a result of electron bombardment.


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