scholarly journals Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination

1992 ◽  
Vol 65 (4) ◽  
pp. 519-522 ◽  
Author(s):  
G Nicoletti ◽  
C De Giovanni ◽  
L Landuzzi ◽  
G Simone ◽  
P Rocchi ◽  
...  
Keyword(s):  
Myogenic Differentiation ◽  
Ionising Radiation

Lumiflavin Enhances the Effects of Ionising Radiation on Ovarian Cancer Stem-Like Cells by Inhibiting Autophagy

2021 ◽  
Vol 21 ◽  
Author(s):  
Meiyuan Wu ◽  
Yangsheng Huang ◽  
Zhaoxia Song ◽  
Ruhui Yang
Keyword(s):  
Ovarian Cancer ◽  
Ovarian Cancer Cell ◽  
Synergistic Effects ◽  
Ionising Radiation ◽  
Adaptive Process ◽  
Cell Vitality ◽  
Ovarian Cancer Cell Lines ◽  
Associated Proteins ◽  
Autophagy Pathway ◽  
Proliferation Ability

Background: The development of cancer stem-like cells (CSCs) is one of the main causes of ovarian cancer tolerance to radiotherapy. Autophagy is an adaptive process by which cells repair damage due to radiation. As a metabolite of riboflavin, lumiflavin can enhance the chemotherapeutic effects of cisplatin on ovarian cancer CSCs. Objective: This study aimed to investigate the synergistic effects of lumiflavin and ionising radiation on ovarian cancer CSCs and explore the association of this metabolite with autophagy. Methods: CSCs of human ovarian cancer cell lines HO8910 were treated with lumiflavin and rapamycin and then subjected to irradiation at a cumulative dose of 8 Gy. Cell proliferation ability, clonal formation ability, apoptosis rate, autophagy changes and autophagy-related protein changes were detected. Results: Lumiflavin and ionising radiation synergistically reduced cell vitality and clone formation and increased the apoptosis of CSCs compared with irradiation alone. In addition, ionising radiation increased autophagy and the expression of associated proteins, whereas lumiflavin reduced those changes in autophagy progression. Moreover, rapamycin, an autophagy inhibitor, was observed to block the synergistic effects of lumiflavin and ionising radiation on CSC apoptosis. Conclusion: Lumiflavin can enhance the effects of ionising radiation on ovarian cancer CSCs. The mechanism by which these effects are exerted is related to blocking the autophagy pathway.

Three-Dimensional Printable Gelatin Hydrogels Incorporating Graphene Oxide to Enable Spontaneous Myogenic Differentiation

2021 ◽  
Vol 10 (4) ◽  
pp. 426-432
Author(s):  
Moon Sung Kang ◽  
Jeon Il Kang ◽  
Phuong Le Thi ◽  
Kyung Min Park ◽  
Suck Won Hong ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Myogenic Differentiation ◽  
Three Dimensional

scholarly journals Surface-directed engineering of tissue anisotropy in microphysiological models of musculoskeletal tissue

2021 ◽  
Vol 7 (11) ◽  
pp. eabe9446 ◽  
Author(s):  
Mark J. Mondrinos ◽  
Farid Alisafaei ◽  
Alex Y. Yi ◽  
Hossein Ahmadzadeh ◽  
Insu Lee ◽  
...  
Keyword(s):  
Muscle Injury ◽  
Cancer Cachexia ◽  
Myogenic Differentiation ◽  
Mechanical Anisotropy ◽  
Musculoskeletal Tissue ◽  
Boundary Constraints ◽  
Cell Contractility ◽  
Engineered Tissue ◽  
Tissue Anisotropy

Here, we present an approach to model and adapt the mechanical regulation of morphogenesis that uses contractile cells as sculptors of engineered tissue anisotropy in vitro. Our method uses heterobifunctional cross-linkers to create mechanical boundary constraints that guide surface-directed sculpting of cell-laden extracellular matrix hydrogel constructs. Using this approach, we engineered linearly aligned tissues with structural and mechanical anisotropy. A multiscale in silico model of the sculpting process was developed to reveal that cell contractility increases as a function of principal stress polarization in anisotropic tissues. We also show that the anisotropic biophysical microenvironment of linearly aligned tissues potentiates soluble factor-mediated tenogenic and myogenic differentiation of mesenchymal stem cells. The application of our method is demonstrated by (i) skeletal muscle arrays to screen therapeutic modulators of acute oxidative injury and (ii) a 3D microphysiological model of lung cancer cachexia to study inflammatory and oxidative muscle injury induced by tumor-derived signals.

scholarly journals Bovine Satellite Cells Isolated after 2 and 5 Days of Tissue Storage Maintain the Proliferative and Myogenic Capacity Needed for Cultured Meat Production

2021 ◽  
Vol 22 (16) ◽  
pp. 8376
Author(s):  
Stig Skrivergaard ◽  
Martin Krøyer Rasmussen ◽  
Margrethe Therkildsen ◽  
Jette Feveile Young
Keyword(s):  
Satellite Cells ◽  
Myogenic Differentiation ◽  
Expression Patterns ◽  
Meat Production ◽  
Positive Trend ◽  
Tissue Storage ◽  
Myogenic Potential ◽  
Cultured Meat ◽  
Start Up ◽  
Myogenic Satellite Cells

Cultured meat is an emerging alternative food technology which aims to deliver a more ethical, sustainable, and healthy muscle-tissue-derived food item compared to conventional meat. As start-up companies are rapidly forming and accelerating this technology, many aspects of this multi-faceted science have still not been investigated in academia. In this study, we investigated if bovine satellite cells with the ability to proliferate and undergo myogenic differentiation could be isolated after extended tissue storage, for the purpose of increasing the practicality for cultured meat production. Proliferation of bovine satellite cells isolated on the day of arrival or after 2 and 5 days of tissue storage were analyzed by metabolic and DNA-based assays, while their myogenic characteristics were investigated using RT-qPCR and immunofluorescence. Extended tissue storage up to 5 days did not negatively affect proliferation nor the ability to undergo fusion and create myosin heavy chain-positive myotubes. The expression patterns of myogenic and muscle-specific genes were also not affected after tissue storage. In fact, the data indicated a positive trend in terms of myogenic potential after tissue storage, although it was non-significant. These results suggest that the timeframe of which viable myogenic satellite cells can be isolated and used for cultured meat production can be greatly extended by proper tissue storage.

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