Effects of α-Amanitin in vivo on RNA Polymerase and Nuclear RNA Synthesis

1972 ◽  
Vol 238 (84) ◽  
pp. 161-164 ◽  
Author(s):  
J. R. TATA ◽  
MARY JO HAMILTON ◽  
D. SHIELDS
Keyword(s):  
Rna Polymerase ◽  
Rna Synthesis ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna

RNA SYNTHESIS IN RAT LIVER AFTER CORTISOL TREATMENT: A POSSIBLE MITOCHONDRIAL-NUCLEAR RELATIONSHIP

1974 ◽  
Vol 77 (2) ◽  
pp. 298-309 ◽  
Author(s):  
A. Maher Mansour ◽  
Sylvan Nass
Keyword(s):  
Rna Polymerase ◽  
Ethidium Bromide ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Ovariectomized Rats ◽  
Saline Control ◽  
Mitochondrial Rna ◽  
Nuclear Rna Synthesis ◽  
Hormone Injection ◽  
Nuclear Rna

ABSTRACT Liver mitochondrial RNA synthesis (as measured by an RNA polymerase assay) in adrenalectomized ovariectomized rats increased (42 %) 1 h after injection of 0.5 mg cortisol/100 g b. wt. Nuclear RNA synthesis increased (130–171 %) 4 h after injection of 4 mg cortisol/100 g but did not increase after treatment with the lower dosage of cortisol after 4 h. There was no effect on the nuclei from either 0.5 or 4 mg cortisol after 1 h. If either ethidium bromide, rifampicin or actinomycin D were injected 10 min before the hormone injection, a complete inhibition of the hormonal action on mitochondrial RNA synthesis was found. On the other hand, nuclear RNA synthesis was partly inhibited if either ethidium bromide or rifampicin preceded the hormone injection. Actinomycin D inhibited nuclear RNA polymerase below the saline control level. Assuming that ethidium bromide and rifampicin act specifically on mitochondrial RNA synthesis, it would appear that there is a mitochondrial component to the RNA synthesis measured in the nucleus after cortisol treatment.

scholarly journals Effect of tannic acid on the synthesis of protein and nucleic acid by rat liver

1969 ◽  
Vol 113 (2) ◽  
pp. 307-313 ◽  
Author(s):  
A. A.-B. Badawy ◽  
Audrey E. White ◽  
G. H. Lathe
Keyword(s):  
Tannic Acid ◽  
Rna Synthesis ◽  
Intraperitoneal Administration ◽  
Nuclear Fraction ◽  
Sequence Of Events ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna ◽  
Tryptophan Pyrrolase

1. As early as 1hr. after the intraperitoneal administration of tannic acid to rats, it could be demonstrated in the liver. At 3hr. the nuclear fraction contained the largest amount of tannic acid. 2. Nuclear RNA synthesis was inhibited in vivo 2hr. after the administration of tannic acid. Induction by cortisol of tryptophan pyrrolase was 90% inhibited at 24hr. 3. Incorporation of [1−14C]leucine into protein by liver slices from treated rats was decreased by 50% after 24hr. Its incorporation into postmitochondrial supernatant from treated animals was not inhibited. Incorporation into slices and postmitochondrial supernatants were inhibited in vitro by tannic acid. 4. The sequence of events: concentration of tannic acid in nuclei, inhibition of nuclear RNA synthesis, inhibition of protein synthesis and production of necrosis, is discussed.

Antibody against a stimulatory factor of RNA polymerase II inhibits nuclear RNA synthesis

Nature ◽  
1979 ◽  
Vol 277 (5692) ◽  
pp. 145-146 ◽  
Author(s):  
KOHJI UENO ◽  
KAZUHISA SEKIMIZU ◽  
DEN'ICHI MIZUNO ◽  
SHUNJI NATORI
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rna Synthesis ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna ◽  
Stimulatory Factor

THE NUCLEAR RNA-SYNTHESIS IN THE ANTERIOR PITUITARY OF RATS

1965 ◽  
Vol 49 (3_Suppl) ◽  
pp. S160 ◽  
Author(s):  
E. Stöcker ◽  
G. Dhom
Keyword(s):  
Anterior Pituitary ◽  
Rna Synthesis ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna

scholarly journals ELECTRON MICROSCOPIC EXAMINATION OF THE SITES OF NUCLEAR RNA SYNTHESIS DURING AMPHIBIAN EMBRYOGENESIS

1965 ◽  
Vol 26 (3) ◽  
pp. 937-958 ◽  
Author(s):  
Shuichi Karasaki
Keyword(s):  
Rna Synthesis ◽  
Developmental Stages ◽  
Early Embryo ◽  
Tail Bud ◽  
Electron Microscopic ◽  
Electron Microscopic Autoradiography ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna ◽  
Chromatin Material ◽  
Labeled Rna

The site of H3-uridine incorporation and the fate of labeled RNA during early embryo-genesis of the newt Triturus pyrrhogaster were studied with electron microscopic autoradiography. Isolated ectodermal and mesodermal tissues from the embryos were treated in H3-uridine for 3 hours and cultured in cold solution for various periods before fixation with OsO4 and embedding in Epon. At the blastula stage, the only structural component of the nucleus seen in electron micrographs is a mass of chromatin fibrils. At the early gastrula stage, the primary nucleoli originate as small dense fibrous bodies within the chromatin material. These dense fibrous nucleoli enlarge during successive developmental stages by the acquisition of granular components 150 A in diameter, which form a layer around them. Simultaneously larger granules (300 to 500 A) appear in the chromatin, and they fill the interchromatin spaces by the tail bud stage. Autoradiographic examination has demonstrated that nuclear RNA synthesis takes place in both the nucleolus and the chromatin, with the former consistently showing more label per unit area than the latter. When changes in the distribution pattern of radioactivity were studied 3 to 24 hours after immersion in isotope at each developmental stage, the following results were obtained. Labeled RNA is first localized in the fibrous region of the nucleolus and in the peripheral region of chromatin material. After longer culture in non-radioactive medium, labeled materials also appear in the granular region of the nucleolus and in the interchromatin areas. Further incubation gives labeling in cytoplasm.

scholarly journals Nuclear RNA synthesis in rat liver during the early stages of chemical carcinogenesis.

1967 ◽  
Vol 21 (1) ◽  
pp. 166-173 ◽  
Author(s):  
E L Godwin ◽  
K R Rees ◽  
J S Varcoe
Keyword(s):  
Rat Liver ◽  
Rna Synthesis ◽  
Chemical Carcinogenesis ◽  
Early Stages ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna

Estrogen Receptor Transformation and Nuclear RNA Synthesis

1973 ◽  
pp. 60-79 ◽  
Author(s):  
Elwood V. Jensen ◽  
Suresh Mohla ◽  
Peter I. Brecher ◽  
Eugene R. DeSombre
Keyword(s):  
Estrogen Receptor ◽  
Rna Synthesis ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna

Control of Nuclear RNA Synthesis in 2-Cell and 4-Cell Mouse Embryos

Nature ◽  
1972 ◽  
Vol 238 (5365) ◽  
pp. 457-459 ◽  
Author(s):  
R. M. BERNSTEIN ◽  
B. B. MUKHERJEE
Keyword(s):  
Rna Synthesis ◽  
Mouse Embryos ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna
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