Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy

2006 ◽  
Vol 1 (2) ◽  
pp. 749-754 ◽  
Author(s):  
Vitali Tugarinov ◽  
Voula Kanelis ◽  
Lewis E Kay
Keyword(s):  
Molecular Weight ◽  
Nmr Spectroscopy ◽  
High Molecular Weight ◽  
Isotope Labeling ◽  
Solution Nmr ◽  
High Molecular Weight Proteins ◽  
Solution Nmr Spectroscopy ◽  
Labeling Strategies

scholarly journals An optimized isotopic labelling strategy of isoleucine-γ2methyl groups for solution NMR studies of high molecular weight proteins

2012 ◽  
Vol 48 (10) ◽  
pp. 1434-1436 ◽  
Author(s):  
Isabel Ayala ◽  
Olivier Hamelin ◽  
Carlos Amero ◽  
Ombeline Pessey ◽  
Michael J. Plevin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Solution Nmr ◽  
Isotopic Labelling ◽  
Nmr Studies ◽  
High Molecular Weight Proteins

Probing Slow Dynamics in High Molecular Weight Proteins by Methyl-TROSY NMR Spectroscopy:  Application to a 723-Residue Enzyme

2004 ◽  
Vol 126 (12) ◽  
pp. 3964-3973 ◽  
Author(s):  
Dmitry M. Korzhnev ◽  
Karin Kloiber ◽  
Voula Kanelis ◽  
Vitali Tugarinov ◽  
Lewis E. Kay
Keyword(s):  
Molecular Weight ◽  
Nmr Spectroscopy ◽  
High Molecular Weight ◽  
Slow Dynamics ◽  
Methyl Trosy ◽  
High Molecular Weight Proteins ◽  
Methyl Trosy Nmr

INTERFERENCE BY SERUM PROTEINS WITH LH RADIOIMMUNOASSAY USING IMMUNOSORBENT

1973 ◽  
Vol 72 (2) ◽  
pp. 235-242 ◽  
Author(s):  
A. M. Reuter ◽  
J. C. Hendrick ◽  
J. Sulon ◽  
P. Franchimont
Keyword(s):  
Molecular Weight ◽  
Human Serum ◽  
High Molecular Weight ◽  
Serum Proteins ◽  
Void Volume ◽  
High Molecular Weight Proteins ◽  
Serum Fractionation ◽  
Covalently Bound

ABSTRACT The percentage of LH* bound to antibodies that have been covalently bound to cellulose is diminished in the presence of LH-free human serum and sera from various species of animals. Serum fractionation studies on Sephadex G 200 show that the greatest interference comes from the proteins eluted in the void volume i. e. the high molecular weight proteins. Specifically, the gamma M globulins and the α2-macroglobulins appear to play an important role, as demonstrated by tests in which these proteins were neutralized by gamma M and α2-macroglobulin antisera.

scholarly journals Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

2010 ◽  
Vol 49 (11) ◽  
pp. 1958-1962 ◽  
Author(s):  
Pierre Gans ◽  
Olivier Hamelin ◽  
Remy Sounier ◽  
Isabel Ayala ◽  
M. Asunción Durá ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Isotopic Labeling ◽  
Spectroscopic Studies ◽  
Methyl Groups ◽  
High Molecular Weight Proteins
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