The NCI60 human tumour cell line anticancer drug screen

Transglutaminases are a family of Ca2(+)-dependent enzymes that catalyse the formation of isopeptide bonds between the side chains of glutamine and lysine residues. The enzymes have been hypothesized to be involved in a wide range of cellular processes, including growth and differentiation and stabilization of the cytoskeleton. The human epidermal carcinoma-cell line, A431 cells, have relatively high amounts of a cytosolic transglutaminase activity that varies upon treatment of the cells with epidermal growth factor. We demonstrate here that this cytosolic activity has the biochemical and immunological properties of a tissue transglutaminase. We also report the purification of this enzyme to apparent homogeneity by a protocol which involves a novel affinity-elution step. Polyclonal antibodies to the transglutaminase were raised and used to identify the enzyme by Western blotting. The availability of purified transglutaminase and antitransglutaminase antibodies will permit further study of the role of this enzyme in the growth of this hormone-responsive human tumour-cell line.

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Induction of changes in the behaviour of endothelial cells by factors produced by a human tumour cell line

Cell Biology International Reports ◽

10.1016/0309-1651(87)90049-x ◽

1987 ◽

Vol 11 (3) ◽

pp. 235 ◽

Cited By ~ 1

Author(s):

M DANIEL ◽

C WALKER ◽

D PUMFORD

Keyword(s):

Endothelial Cells ◽

Cell Line ◽

Tumour Cell ◽

Tumour Cell Line ◽

Human Tumour ◽

Human Tumour Cell Line

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Hypersensitivity of a Human Tumour Cell Line to Very Low Radiation Doses

International Journal of Radiation Biology ◽

10.1080/09553009314450831 ◽

1993 ◽

Vol 63 (5) ◽

pp. 639-650 ◽

Cited By ~ 79

Author(s):

P. Lambin ◽

B. Marples ◽

B. Fertil ◽

E.P. Malaise ◽

M.C. Joiner

Keyword(s):

Cell Line ◽

Tumour Cell ◽

Tumour Cell Line ◽

Radiation Doses ◽

Human Tumour ◽

Human Tumour Cell Line ◽

Low Radiation Doses

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Cytotoxicity and activation of CB1954 in a human tumour cell line

Biochemical Pharmacology ◽

10.1016/0006-2952(91)90100-j ◽

1991 ◽

Vol 41 (9) ◽

pp. 1293-1298 ◽

Cited By ~ 11

Author(s):

Andrew Suntes ◽

John Baer ◽

Kenneth D. Bagshawe

Keyword(s):

Cell Line ◽

Tumour Cell ◽

Tumour Cell Line ◽

Human Tumour ◽

Human Tumour Cell Line

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Cytotoxic Cholestane and Pregnane Glycosides from Tribulus macropterus

Zeitschrift für Naturforschung C ◽

10.1515/znc-2007-5-602 ◽

2007 ◽

Vol 62 (5-6) ◽

pp. 319-325 ◽

Cited By ~ 8

Author(s):

El-Sayed S. Abdel-Hameed ◽

Hanan A. El-Nahas ◽

Eman A. El-Wakil

Keyword(s):

Cytotoxic Activity ◽

Cell Line ◽

Tumour Cell ◽

Methanol Extract ◽

Methanolic Extract ◽

Chemical Data ◽

Tumour Cell Line ◽

Human Tumour ◽

Human Tumour Cell Line ◽

Chromatographic Techniques

The methanol extract of the whole parts of Tribulus macropterus Boiss. (family Zygophyllaceae) showed cytotoxic activity against a human tumour cell line (hepatocyte generation 2, HepG2) (IC50 = 2.9 μg/ml). The n-butanolic fraction obtained from successive fractionation of the methanolic extract exhibited activity against HepG2 (IC50 = 2.6 μg/ml). Therefore, this fraction was subjected to separation using different chromatographic techniques. Five compounds, 1-5, were isolated and identified as: (22S,25S)-16β,22,26-trihydroxy-cholest-4- en-3-one-16-O-β-D-glucopyranosyl-(1→3)-β-D-xylopyranoside (1), (22S,25S)-16β,22,26-trihydroxy- cholest-4-en-3-one-16-O-β-D-glucopyranosyl-(1→3)-β-d-glucopyranoside (2), sucrose (3), d-pinitol (4) and 3β-hydroxy-5α-pregn-16(17)en-20-one-3-O-β-D-xylopyranosyl-(1→2)- [β-d-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-galactopyranoside (5) on the basis of spectroscopic and chemical data. The three steroidal compounds 1, 2 and 5 were also tested against the same cell line HepG2 and their IC50 values were 2.4, 2.2 and 1.1 μg/ml, respectively.

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