scholarly journals High-speed volumetric imaging of neuronal activity in freely moving rodents

2018 ◽  
Vol 15 (6) ◽  
pp. 429-432 ◽  
Author(s):  
Oliver Skocek ◽  
Tobias Nöbauer ◽  
Lukas Weilguny ◽  
Francisca Martínez Traub ◽  
Chuying Naomi Xia ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
High Speed ◽  
Volumetric Imaging ◽  
Freely Moving

scholarly journals Author Correction: High-speed volumetric imaging of neuronal activity in freely moving rodents

2018 ◽  
Vol 15 (6) ◽  
pp. 469-469 ◽  
Author(s):  
Oliver Skocek ◽  
Tobias Nöbauer ◽  
Lukas Weilguny ◽  
Francisca Martínez Traub ◽  
Chuying Naomi Xia ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
High Speed ◽  
Volumetric Imaging ◽  
Freely Moving

scholarly journals High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity

2021 ◽  
Vol 118 (8) ◽  
pp. 081104
Author(s):  
Andrew J. Bower ◽  
Carlos Renteria ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
High Speed ◽  
Label Free ◽  
Two Photon ◽  
Metabolic Transients ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals High-speed Fly-Scan Volumetric Imaging

2018 ◽  
Vol 24 (S1) ◽  
pp. 498-499 ◽  
Author(s):  
Doga Gursoy
Keyword(s):  
High Speed ◽  
Volumetric Imaging

scholarly journals Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice

2019 ◽  
Vol 13 ◽  
Author(s):  
Jiwei Yao ◽  
Qianwei Li ◽  
Xianping Li ◽  
Han Qin ◽  
Shanshan Liang ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Simultaneous Measurement ◽  
Freely Moving

scholarly journals Distal convoluted tubule sexual dimorphism revealed by advanced 3D imaging

2020 ◽  
Vol 319 (5) ◽  
pp. F754-F764
Author(s):  
Ebrahim Tahaei ◽  
Richard Coleman ◽  
Turgay Saritas ◽  
David H. Ellison ◽  
Paul A. Welling
Keyword(s):  
Sexual Dimorphism ◽  
High Speed ◽  
Distal Convoluted Tubule ◽  
Volumetric Imaging ◽  
Female Mice ◽  
Cellular Density ◽  
The Face ◽  
Antibody Labeling ◽  
Male Subjects ◽  
Insight Into

The thiazide-sensitive Na+-Cl− cotransporter (NCC) is more abundant in kidneys of female subjects than of male subjects. Because morphological remodeling of the distal convoluted tubule (DCT) is dependent on NCC activity, it has been generally assumed that there is a corresponding sexual dimorphism in the structure of the DCT, leading to a larger female DCT. Until now, this has never been directly examined. Here, optical clearing techniques were combined with antibody labeling of DCT segment markers, state-of-the-art high-speed volumetric imaging, and analysis tools to visualize and quantify DCT morphology in male and female mice and study the DCT remodeling response to furosemide. We found an unexpected sex difference in the structure of the DCT. Compared with the male mice, female mice had a shorter DCT, a higher cellular density of NCC, and a greater capacity to elongate in response to loop diuretics. Our study revealed a sexual dimorphism of the DCT. Female mice expressed a greater density of NCC transporters in a shorter structure to protect Na+ balance in the face of greater basal distal Na+ delivery yet have a larger reserve and structural remodeling capacity to adapt to unique physiological stresses. These observations provide insight into mechanisms that may drive sex differences in the therapeutic responses to diuretics.

scholarly journals Imaging localized neuronal activity at fast time scales through biomechanics

2019 ◽  
Vol 5 (4) ◽  
pp. eaav3816 ◽  
Author(s):  
Samuel Patz ◽  
Daniel Fovargue ◽  
Katharina Schregel ◽  
Navid Nazari ◽  
Miklos Palotai ◽  
...  
Keyword(s):  
Time Scales ◽  
Neuronal Activity ◽  
High Speed ◽  
Fast Time ◽  
Regional Patterns ◽  
Cortical Input ◽  
Brain Biomechanics ◽  
Neuronal Processes ◽  
High Level

Mapping neuronal activity noninvasively is a key requirement for in vivo human neuroscience. Traditional functional magnetic resonance (MR) imaging, with a temporal response of seconds, cannot measure high-level cognitive processes evolving in tens of milliseconds. To advance neuroscience, imaging of fast neuronal processes is required. Here, we show in vivo imaging of fast neuronal processes at 100-ms time scales by quantifying brain biomechanics noninvasively with MR elastography. We show brain stiffness changes of ~10% in response to repetitive electric stimulation of a mouse hind paw over two orders of frequency from 0.1 to 10 Hz. We demonstrate in mice that regional patterns of stiffness modulation are synchronous with stimulus switching and evolve with frequency. For very fast stimuli (100 ms), mechanical changes are mainly located in the thalamus, the relay location for afferent cortical input. Our results demonstrate a new methodology for noninvasively tracking brain functional activity at high speed.

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