Transcriptome profiling of interaction effects of soybean cyst nematodes and soybean aphids on soybean

AbstractSoybean cyst nematode (SCN), Heterodera glycines, is one of the most important pests in soybean production worldwide. In China, 11 different races of SCN, including a newly identified race ‘X12’ with super-virulence, have been surveyed and found to be distributed in 22 provinces. Among them, races 1, 3 and 4 are dominant in the two principal soybean-producing areas, Northeast China and Huanghuaihai Valley, causing over 120 million dollars of annual yield loss. Rapid and reliable PCR-based approaches have been developed for the molecular diagnosis of SCN. High-throughput methods for the identification of soybean resistance against SCN are also developed with specific single nucleotide polymorphism markers by using Kompetitive Allele Specific PCR technology. Over 10,000 soybean germplasm sources were evaluated for their SCN resistance, and 28 SCN-resistant soybean accessions were selected to construct an applied core collection, which has been used for soybean breeding in China. Recently, the genome sequences of SCN and soybean are publically available, and two major SCN-resistant genes (rhg1 and Rhg4) have been identified in soybean, which greatly facilitate the researches on SCN virulence and soybean resistance, and also soybean resistance breeding against SCN. However, the management of SCN still faces many bottlenecks, for instance, the single resistance genes in soybean cultivars can be easily overcome by new SCN races; the identified resistance genes are inadequate to meet the practical breeding needs; and our understanding of the mechanisms of SCN virulence and soybean resistance to SCN are limited. SCN is a destructive threat to soybean production throughout the world including China. In this review, the major progress on soybean SCN is summarized, mainly focusing on the recent research progress in SCN, soybean resistance to SCN and integrated management of SCN in China, and aiming at a better understanding of the current SCN research status and prospects for future work.

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Genetic Relationships among Soybean Plant Introductions with Resistance to Soybean Cyst Nematodes

Crop Science ◽

10.2135/cropsci1997.0011183x003700060048x ◽

1997 ◽

Vol 37 (6) ◽

pp. 1966-1972 ◽

Cited By ~ 32

Author(s):

B. W. Diers ◽

H. T. Skorupska ◽

A. P. Rao‐Arelli ◽

S. R. Cianzio

Keyword(s):

Genetic Relationships ◽

Soybean Plant ◽

Cyst Nematodes ◽

Plant Introductions ◽

Soybean Cyst Nematodes

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Response of soybean cyst nematodes to herbicide application on soybeans

10.31274/rtd-180813-10149 ◽

1995 ◽

Author(s):

Brian Charles Levene

Keyword(s):

Herbicide Application ◽

Cyst Nematodes ◽

Soybean Cyst Nematodes

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Selection and Reproduction of Soybean Cyst Nematodes on Resistant Soybeans 1

Crop Science ◽

10.2135/cropsci1982.0011183x002200010017x ◽

1982 ◽

Vol 22 (1) ◽

pp. 78-80 ◽

Cited By ~ 10

Author(s):

J. McCann ◽

V. D. Luedders ◽

V. H. Dropkin

Keyword(s):

Cyst Nematodes ◽

Soybean Cyst Nematodes

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Status of soybean cyst nematodes and integrated management in China.

10.1079/9781789247541.0016 ◽

2021 ◽

pp. 111-116

Author(s):

De-liang Peng

Keyword(s):

Heterodera Glycines ◽

Integrated Management ◽

Management Strategies ◽

Economic Importance ◽

Cyst Nematode ◽

Cyst Nematodes ◽

Future Outlook ◽

Nematode Management ◽

Soybean Cyst Nematodes ◽

Damage Symptoms

Abstract This chapter focuses on the host range, geographical distribution, economic importance, damage symptoms and biology and life cycle of the soyabean cyst nematode Heterodera glycines in China. Some information on their interactions with other nematodes and pathogens, efficacy and optimization of some recommended integrated nematode management systems and future outlook and research requirements for nematode management strategies are also presented.

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Genome-wide identification of soybean microRNA responsive to soybean cyst nematodes infection by deep sequencing

BMC Genomics ◽

10.1186/s12864-017-3963-4 ◽

2017 ◽

Vol 18 (1) ◽

Cited By ~ 24

Author(s):

Bin Tian ◽

Shichen Wang ◽

Timothy C. Todd ◽

Charles D. Johnson ◽

Guiliang Tang ◽

...

Keyword(s):

Deep Sequencing ◽

Cyst Nematodes ◽

Genome Wide ◽

Soybean Cyst Nematodes

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Response of soybean cyst nematodes and soybeans (Glycine max) to herbicides

Weed Science ◽

10.1017/s0043174500090512 ◽

1998 ◽

Vol 46 (2) ◽

pp. 264-270 ◽

Cited By ~ 15

Author(s):

Brian C. Levene ◽

Micheal D.K. Owen ◽

Gregory L. Tylka

Keyword(s):

Glycine Max ◽

Nonionic Surfactant ◽

Untreated Control ◽

Soybean Cyst Nematode ◽

Cyst Nematode ◽

Additive Effect ◽

Cyst Nematodes ◽

Population Densities ◽

Soybean Cyst Nematodes

The effect of herbicides applied to V3 soybeans on race 3 soybean cyst nematode (SCN) reproduction and glyceollin production in roots was measured. Soybeans were treated postemergence with 1 × and 2 × rates of herbicides plus adjuvants or with adjuvants alone, and SCN development was measured. Acifluorfen, bentazon, lactofen, crop oil concentrate (COC), and nonionic surfactant (NIS) applications reduced SCN egg population densities 50 to 60% compared with the untreated control 4 and 8 wk after application. The SCN reproduction on plants treated with fluazifop-P, sethoxydim, and imazethapyr was similar to the untreated control. Crop oil concentrate or NIS applications alone were as effective as acifluorfen, bentazon, or lactofen applications for reducing SCN reproduction. However, no additive effect of adjuvant-herbicide combinations was observed, nor did herbicide rate affect SCN reproduction. Treatments reduced SCN reproduction only when applied to soybeans and had no effect on SCN reproduction when applied directly to the soil. No treatment stimulated SCN reproduction relative to the untreated control. Soybeans treated with COC, NIS, acifluorfen, and bentazon also had more glyceollin detected than the untreated control. Herbicide-induced glyceollin production may have increased the resistance of soybean to SCN.

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Nutritional Requirements of Soybean Cyst Nematodes

Soybean - Pest Resistance ◽

10.5772/54247 ◽

2013 ◽

Author(s):

Steven C. ◽

James A. ◽

Patricia Donal

Keyword(s):

Nutritional Requirements ◽

Cyst Nematodes ◽

Soybean Cyst Nematodes

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In Vitro Screening of a Culturable Soybean Cyst Nematode Cyst Mycobiome for Potential Biological Control Agents and Biopesticides

Phytopathology ◽

10.1094/phyto-01-20-0015-r ◽

2020 ◽

Vol 110 (8) ◽

pp. 1388-1397 ◽

Cited By ~ 2

Author(s):

Deepak Haarith ◽

Dong-gyu Kim ◽

Noah B. Strom ◽

Senyu Chen ◽

Kathryn E. Bushley

Keyword(s):

Biological Control ◽

Soybean Cyst Nematode ◽

Fluorescent Microscopy ◽

Biological Control Agents ◽

Cyst Nematodes ◽

Egg Hatch ◽

High Performing ◽

Soybean Cyst Nematodes ◽

Egg Parasitism

Fungal biological control of soybean cyst nematodes (SCN) is an important component of integrated pest management for soybean. However, very few fungal biological control agents are available in the market. In this study, we have screened fungi previously isolated from SCN cysts over 3 years from a long-term crop rotation field experiment for their ability to antagonize SCN using (i) parasitism, (ii) egg hatch inhibition, and (iii) J2 mortality. We evaluated egg parasitism using an in-vitro egg parasitism bioassays and scored parasitism using the egg parasitic index (EPI) and fluorescent microscopy. The ability of these fungi to produce metabolites causing egg hatch inhibition and J2 mortality was assessed in bioassays using filter-sterilized culture filtrates. We identified 10 high-performing isolates each for egg parasitism and toxicity toward SCN eggs and J2s and repeated the tests after storage for 1 year of cryopreservation at −80°C to validate the durability of biocontrol potential of the chosen 20 isolates. Although the parasitic ability changed slightly for the majority of strains after cryopreservation, they still scored 5/10 on EPI scales. There were no differences in the ability of fungi to produce antinemic metabolites after cryopreservation. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

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