Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms

The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein. The samples were provided by the Vladimir veterinary service. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely - 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region. Eight studied isolates from the Nizhny Novgorod and Ryazan regions were attributed to the Eurasian phylogenetic group.

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Molecular characterization of field isolates of infectious bursal disease virus from three decades, 1987–2018, reveals a distinct genotypic subgroup in Vietnam

Archives of Virology ◽

10.1007/s00705-019-04287-w ◽

2019 ◽

Vol 164 (8) ◽

pp. 2137-2145

Author(s):

Xuyen Thi Kim Le ◽

Huong Thi Thanh Doan ◽

Roan Thi Do ◽

Thanh Hoa Le

Keyword(s):

Molecular Characterization ◽

Disease Virus ◽

Infectious Bursal Disease Virus ◽

Infectious Bursal Disease ◽

Field Isolates ◽

Bursal Disease

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Characterization of Natural Isolates of Bipolaris maydis Associated with Mating Types, Genetic Diversity, and Pathogenicity in Fujian Province, China

Plant Disease ◽

10.1094/pdis-03-19-0650-re ◽

2020 ◽

Vol 104 (2) ◽

pp. 323-329

Author(s):

Yuli Dai ◽

Lin Gan ◽

Hongchun Ruan ◽

Niuniu Shi ◽

Yixin Du ◽

...

Keyword(s):

Genetic Diversity ◽

Mating Type ◽

Dna Polymorphism ◽

Sweet Corn ◽

Issr Markers ◽

Bipolaris Maydis ◽

Fujian Province ◽

Field Isolates ◽

Rational Distribution

Due to the natural destructiveness and persistence of the southern corn leaf blight (SCLB) fungus Bipolaris maydis (Nisikado et Miyake) Shoem, the characterization of B. maydis field isolates is essential to guide the rational distribution of resistant materials in corn-growing regions. In the present study, 102 field isolates collected from seven locations covering the entire region of Fujian Province, China, were assessed for mating type distribution, genetic diversity, and pathogenicity toward local sweet corn cultivars. Mating type detection via polymerase chain reaction indicated that 36.3 and 63.7% of isolates were MAT1-1 and MAT1-2, respectively; more than 80% of these isolates were confirmed using cross assays with known mating type isolates. Thirteen intersimple sequence repeat (ISSR) markers within and among two mating type populations revealed a high level of DNA polymorphism for all combined isolates and between MAT1-1 and MAT1-2 populations. The MAT1-2 population was more diverse based on DNA polymorphism than the MAT1-1 population. The value of GST was 0.0070, ranging from 0.0399 to 0.3044 based on analysis of combined isolates and individual regional populations, respectively, suggesting the presence of genetic differentiation in the two mating type populations from different locations. Pathogenicity assays revealed that both MAT1-1 and MAT1-2 populations were pathogenic to all 11 local sweet corn cultivars tested in this study. The potential of sexual reproduction, existence of genetic diversity in the two mating type populations, and pathogenicity suggest that B. maydis populations have independently clonally adapted under natural field conditions during corn cultivation.

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Characterization of a Plasmodium falciparum PHISTc protein, PF3D7_0801000, in blood- stage malaria parasites

Parasitology International ◽

10.1016/j.parint.2020.102240 ◽

2021 ◽

Vol 80 ◽

pp. 102240

Author(s):

Hikaru Nagaoka ◽

Bernard N. Kanoi ◽

Masayuki Morita ◽

Takahiro Nakata ◽

Nirianne M.Q. Palacpac ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Blood Stage ◽

Malaria Parasites ◽

Blood Stage Malaria

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Characterization of the Duffy-Binding-Like Domain of Plasmodium falciparum Blood-Stage Antigen 332

Malaria Research and Treatment ◽

10.4061/2011/671439 ◽

2011 ◽

Vol 2011 ◽

pp. 1-14 ◽

Cited By ~ 4

Author(s):

Sandra Nilsson ◽

Kirsten Moll ◽

Davide Angeletti ◽

Letusa Albrecht ◽

Inari Kursula ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Immunofluorescence Microscopy ◽

Homology Model ◽

Cross Reactivity ◽

Blood Stage ◽

Conserved Domain ◽

Erythrocyte Binding ◽

High Degree ◽

Degree Of Similarity

Studies on Pf332, a major Plasmodium falciparum blood-stage antigen, have largely been hampered by the cross-reactive nature of antibodies generated against the molecule due to its high content of repeats, which are present in other malaria antigens. We previously reported the identification of a conserved domain in Pf332 with a high degree of similarity to the Duffy-binding-like (DBL) domains of the erythrocyte-binding-like (EBL) family. We here describe that antibodies towards Pf332-DBL are induced after repeated exposure to P. falciparum and that they are acquired early in life in areas of intense malaria transmission. Furthermore, a homology model of Pf332-DBL was found to be similar to the structure of the EBL-DBLs. Despite their similarities, antibodies towards Pf332-DBL did not display any cross-reactivity with EBL-proteins as demonstrated by immunofluorescence microscopy, Western blotting, and peptide microarray. Thus the DBL domain is an attractive region to use in further studies on the giant Pf332 molecule.

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