scholarly journals MOLECULAR-GENETIC CHARACTERIZATION OF FIELD ISOLATES OF RABIES VIRUS IDENTIFIED IN THE TERRITORY OF VLADIMIR, MOSCOW, TVER, NIZHNY NOVGOROD AND RYAZAN REGIONS

2017 ◽  
Vol 62 (3) ◽  
pp. 101-108
Author(s):  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
A. M. Gulyukin ◽  
A. A. Shabeykin ◽  
I. V. Polyakova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rabies Virus ◽  
Molecular Genetic ◽  
Phylogenetic Group ◽  
Molecular Genetic Study ◽  
Field Isolates ◽  
Vladimir Region ◽  
Pcr Products ◽  
Molecular Genetic Characterization

The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein. The samples were provided by the Vladimir veterinary service. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely - 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region. Eight studied isolates from the Nizhny Novgorod and Ryazan regions were attributed to the Eurasian phylogenetic group.

Molecular genetic characterization of rabies virus glycoprotein gene sequences from rabid dogs in Bangkok and neighboring provinces in Thailand, 2013–2014

2016 ◽  
Vol 161 (5) ◽  
pp. 1261-1271 ◽  
Author(s):  
Surachet Benjathummarak ◽  
Chanon Fa-ngoen ◽  
Chonlatip Pipattanaboon ◽  
Khwanchit Boonha ◽  
Pongrama Ramasoota ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Genetic Characterization ◽  
Molecular Genetic ◽  
Gene Sequences ◽  
Rabies Virus Glycoprotein ◽  
Virus Glycoprotein ◽  
Glycoprotein Gene ◽  
Molecular Genetic Characterization

scholarly journals Characterization of the Full-Length Genome Sequences and Phylogenetic Analysis of HDV Strains Isolated from Patients with Chronic HBV and HDV Infection in Kyrgyz Republic

2020 ◽  
pp. 124-132
Author(s):  
Yu. V. Ostankova ◽  
K. A. Nogoybaeva ◽  
E. B. Zueva ◽  
K. T. Kasymbekova ◽  
S. T. Tobokalova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Molecular Genetic ◽  
Kyrgyz Republic ◽  
Genotype 1 ◽  
Hepatitis D ◽  
Hdv Infection ◽  
Molecular Genetic Characterization ◽  
Hdv Genotype

Objective. The purpose of our work was molecular genetic characterization of the hepatitis D virus isolates, circulating in the region with high prevalence of HBV + HDV super-infection. Materials and methods. The study material was 64 blood serum samples obtained from Kyrgyz Republic residents - patients with chronic viral hepatitis B+D. The hepatitis D virus complete genomes were sequenced, followed by phylogenetic analysis. Results and discussion. Based on the phylogenetic analysis of 64 HDV samples, it was shown that HDV genotype 1 (96.9 %) predominates in the examined group compared with HDV genotype 2 (3.1 %). Sequences were submitted to GenBank under access No MN984407 through MN984470. When assessing the genetic variability over the examined HDV genotype 1 samples, the maximum genetic distance was 12,49 %, and the minimum – 7,41 %. Within individual clusters, the genetic distance averaged from 2.6 % to 8.5 %. Among the sequences in GenBank, the closest resemblance to the HDV-2 Kyr41 and Kyr43 samples (nucleotide identity was 92.31 % and 89.57 %, respectively) was shown for the virus described earlier in Yakutia (AJ309880). To study the genetic relationships between the analyzed HDV genotype 1 strains in comparison with the HDV reference sequences, the predicted amino acid sequence was studied (111–214). Although hepatitis B preventive measures, including vaccination, have reduced the hepatitis D incidence, there is no effective way to prevent HDV infection in HBV carriers in endemic areas. The HDV sequence molecular-genetic characterization in this study, as well as the viral genomic sequence phylogenetic analysis, will help identify pathogen transmission pathways to control and / or prevent the spread of infection.

scholarly journals FEATURES OF EPISOTIC PROCESS AND MOLECULAR-GENETIC CHARACTERISTICS OF VIRUS ISOLATES OF RABIES IN TVER REGION

2018 ◽  
Vol 63 (3) ◽  
pp. 115-123
Author(s):  
A. M. Gulukin ◽  
A. A. Shabejkin ◽  
V. V. Makarov ◽  
O. N. Zaykova ◽  
T. V. Grebennikova ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rabies Virus ◽  
Molecular Genetic ◽  
Phylogenetic Group ◽  
N Gene ◽  
Genetic Characteristics ◽  
Temporal Features ◽  
Epizootic Process ◽  
Tver Region ◽  
Virus Isolates

Rabies epidemic situation in the Tver Region has been studied. Animals of different species that had confirmed clinical rabies were statistically analyzed. It was established that the features of the course of epizootics in the Tver region correspond to the regularities characteristic of rabies of the natural-focal type. As a result of sequencing of the rabies virus N gene and phylogenetic analysis, the isolates studied were assigned to the central phylogenetic group. With the help of the geoinformatic system, nosological maps of the Tver region were obtained and the spatial- temporal features of the course of the epizootic process of rabies infection were studied.

scholarly journals Metabolic Flux in Both the Purine Mononucleotide and Histidine Biosynthetic Pathways Can Influence Synthesis of the Hydroxymethyl Pyrimidine Moiety of Thiamine in Salmonella enterica

2002 ◽  
Vol 184 (22) ◽  
pp. 6130-6137 ◽  
Author(s):  
Shara Allen ◽  
Julie L. Zilles ◽  
Diana M. Downs
Keyword(s):  
Metabolic Flux ◽  
Genetic Characterization ◽  
Molecular Genetic ◽  
Thiamine Pyrophosphate ◽  
Wild Type ◽  
Regulatory Pathway ◽  
Informational Suppressors ◽  
Pyrimidine Moiety ◽  
Molecular Genetic Characterization

ABSTRACT Together, the biosyntheses of histidine, purines, and thiamine pyrophosphate (TPP) contain examples of convergent, divergent, and regulatory pathway integration. Mutations in two purine biosynthetic genes (purI and purH) affect TPP biosynthesis due to flux through the purine and histidine pathways. The molecular genetic characterization of purI mutants and their respective pseudorevertants resulted in the conclusion that <1% of the wild-type activity of the PurI enzyme was sufficient for thiamine but not for purine synthesis. The respective pseudorevertants were found to be informational suppressors. In addition, it was shown that accumulation of the purine intermediate aminoimidazole carboxamide ribotide inhibits thiamine synthesis, specifically affecting the conversion of aminoimidazole ribotide to hydroxymethyl pyrimidine.

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