scholarly journals U-DCS: characterization of the first permanent human dendritic sarcoma cell line

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kevin Mellert ◽  
Julian Benckendorff ◽  
Frank Leithäuser ◽  
Katarzyna Zimmermann ◽  
Peter Wiegand ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Cell Line ◽  
S100 Protein ◽  
Sarcoma Cell ◽  
Vimentin Expression ◽  
Sarcoma Cell Line ◽  
Cell Sarcoma ◽  
Dendritic Cell Sarcoma ◽  
Functional Features

AbstractA dendritic cell sarcoma cell line, U-DCS, was established from a dendritic cell sarcoma in a 53-year-old Caucasian male patient. Since its establishment, U-DCS has maintained stable phenotypic characteristics in vitro and has a doubling time of approximately 2 days under standard culture conditions. U-DCS is growing with typical dendritic cell morphology in tissue and expresses the dendritic cell sarcoma immunophenotypic markers S100 protein, MHCI, MHCII, and vimentin. Expression analysis revealed transcripts for the toll-like receptors TLR3, -4, -9 and DDX58 (RIG-I), but not for TLR2. U-DCS shows functional features of dendritic cells with the ability of phagocytosis and antigen-specific T cell stimulation. Karyotype-, CGH-, and mFISH analysis point to a chromosomal instability and a hypotetraploid karyotype with approximately 130 chromosomes. U-DCS is the first immortalized human dendritic cell sarcoma cell line and has some morphological and functional features of dendritic cells without dependency on growth factors.

scholarly journals Establishment and Characterization of a Small Round Cell Sarcoma Cell Line, SCCH-196, with t(11;22)(q24;q12)

1989 ◽  
Vol 80 (9) ◽  
pp. 861-865 ◽  
Author(s):  
Chieko Homma ◽  
Yasuhiko Kaneko ◽  
Ki-ichi Sekine ◽  
Shin-ichi Hara ◽  
Jun-ichi Hata ◽  
...  
Keyword(s):  
Cell Line ◽  
Round Cell ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Cell Sarcoma ◽  
Small Round Cell

Establishment of a new continuous clear cell sarcoma cell line

1997 ◽  
Vol 431 (1) ◽  
pp. 45-51 ◽  
Author(s):  
H. Hiraga ◽  
Takayuki Nojima ◽  
Syuiti Abe ◽  
Katsushige Yamashiro ◽  
Shinya Yamawaki ◽  
...  
Keyword(s):  
Cell Line ◽  
Clear Cell ◽  
Clear Cell Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Cell Sarcoma

Establishment and characterization of a new human clear-cell sarcoma cell-line, HS-MM

1993 ◽  
Vol 169 (3) ◽  
pp. 317-322 ◽  
Author(s):  
Hiroshi Sonobe ◽  
Mutsuo Furihata ◽  
Jun Iwata ◽  
Yuji Ohtsuki ◽  
Hiroo Mizobuchi ◽  
...  
Keyword(s):  
Cell Line ◽  
Clear Cell ◽  
Clear Cell Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Cell Sarcoma

Interdigitating Dendritic Cell Sarcoma of the Duodenum With Rapidly Fatal Course: A Case Report and Review of the Literature

2006 ◽  
Vol 130 (2) ◽  
pp. 205-208 ◽  
Author(s):  
Hassan Kanaan ◽  
Jaudah Al-Maghrabi ◽  
Ayman Linjawi ◽  
Amira Al-Abbassi ◽  
Abdelghani Dandan ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Epigastric Pain ◽  
Final Diagnosis ◽  
Lymph Node Involvement ◽  
Whipple Procedure ◽  
Upper Gastrointestinal Endoscopy ◽  
Cell Sarcoma ◽  
Dendritic Cell Sarcoma ◽  
Interdigitating Dendritic Cell Sarcoma

Abstract Interdigitating dendritic cell sarcoma is an extremely rare malignancy derived from antigen-presenting cells. Dendritic cells constitute a heterogeneous group of cells, which includes Langerhans cells, dermal dendrocytes, follicular dendritic cells, and interdigitating dendritic cells present in lymphoid and nonlymphoid organs. We report the case of a 36-year-old woman who presented with epigastric pain, projectile vomiting, and significant weight loss. Upper gastrointestinal endoscopy showed a duodenal lesion; a biopsy of the lesion was taken and was diagnosed as sarcoma. She underwent a Whipple procedure. A final diagnosis of interdigitating dendritic cell sarcoma was made, with liver and peripancreatic lymph node involvement. The patient deteriorated rapidly and died 4 months later. Although interdigitating dendritic cell sarcoma of the duodenum is extremely rare, we think it should be included in the differential diagnosis of unusual spindle cell tumors with a rich lymphocytic infiltrate.

Regulation of the exopolysaccharide from an anamorph of Cordyceps sinensis on dendritic cell sarcoma (DCS) cell line

2012 ◽  
Vol 52 (2) ◽  
pp. 687-694 ◽  
Author(s):  
Dan Song ◽  
Zhenyue He ◽  
Chenhao Wang ◽  
Fengjiao Yuan ◽  
Ping Dong ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Cell Line ◽  
Cordyceps Sinensis ◽  
Cell Sarcoma ◽  
Dendritic Cell Sarcoma

scholarly journals Establishment of a novel clear cell sarcoma cell line (Hewga-CCS), and investigation of the antitumor effects of pazopanib on Hewga-CCS

BMC Cancer ◽  
2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Hidetatsu Outani ◽  
Takaaki Tanaka ◽  
Toru Wakamatsu ◽  
Yoshinori Imura ◽  
Kenichiro Hamada ◽  
...  
Keyword(s):  
Cell Line ◽  
Clear Cell ◽  
Clear Cell Sarcoma ◽  
Sarcoma Cell ◽  
Antitumor Effects ◽  
Sarcoma Cell Line ◽  
Cell Sarcoma

Modulation of glucose transport by parathyroid hormone and insulin in UMR 106–01, a clonal rat osteogenic sarcoma cell line

1995 ◽  
Vol 14 (2) ◽  
pp. 263-275 ◽  
Author(s):  
D M Thomas ◽  
S D Rogers ◽  
M W Sleeman ◽  
G M Pasquini ◽  
F R Bringhurst ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cell Line ◽  
Glucose Transport ◽  
Transport System ◽  
Osteogenic Sarcoma ◽  
Regulatory Subunit ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Glucose Transport System ◽  
Umr 106

ABSTRACT This study characterizes the actions of insulin and parathyroid hormone (PTH) on the glucose transport system in the rat osteogenic sarcoma cell line UMR 106–01, which expresses a number of features of the osteoblast phenotype. Using [1,2-3H]2-deoxyglucose (2-DOG) as a label, UMR 106–01 cells were shown to possess a glucose transport system which was enhanced by insulin. In contrast, PTH influenced glucose transport in a biphasic manner with a stimulatory effect at 1 h and a more potent inhibitory effect at 16 h on basal and insulin-stimulated 2-DOG transport. To explore the mechanism of PTH action, a direct agonist of cAMP-dependent protein kinase (PKA) was tested. 8-Bromo-cAMP had no acute stimulatory effect but inhibited basal and insulin-stimulated 2-DOG transport at 16 h. This result suggested that the prolonged, but not the acute, effect of PTH was mediated by the generation of cAMP. Further studies with the cell line UMR 4–7, a UMR 106–01 clone stably transfected with an inducible mutant inactive regulatory subunit of PKA, confirmed that the inhibitory but not the stimulatory effect of PTH was mediated by the PKA pathway. Northern blot data indicated that the prolonged inhibitory effects of PTH and 8-bromo-cAMP on glucose transport were likely to be mediated in part by reduction in the levels of GLUT1 (HepG2/brain glucose transporter) mRNA.

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