scholarly journals The effect of the use of commercial tempeh starter on the diversity of Rhizopus tempeh in Indonesia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wellyzar Sjamsuridzal ◽  
Mangunatun Khasanah ◽  
Rela Febriani ◽  
Yura Vebliza ◽  
Ariyanti Oetari ◽  
...  

AbstractAt present, only a single Rhizopus species, R. microsporus, can be found in fresh tempeh produced in Java, Indonesia. The loss of diversity of Rhizopus in tempeh has been associated with the widespread use of commercial tempeh starter in Indonesia since the 2000s. However, the identities of the previous Rhizopus strains associated with tempeh, which have been preserved in a culture collection in Indonesia, have not been verified. The present study aimed to verify the identities of 22 Rhizopus strains isolated from tempeh produced using the traditional tempeh starters from the 1960s to the 2000s. Phylogenetic analysis based on the ITS regions in the rRNA gene sequence data, revealed that the Rhizopus strains belonged to the species R. arrhizus (five strains); R. delemar (14 strains); and R. microsporus (three strains). Verification of the identities of these Rhizopus strains in the present study confirmed the loss of diversity of Rhizopus species in tempeh produced in Indonesia, particularly in Java. Our findings confirmed that the morphological changes in Rhizopus species isolated from tempeh as a result of centuries of domestication.

2004 ◽  
Vol 54 (2) ◽  
pp. 493-497 ◽  
Author(s):  
Brian J. Henson ◽  
Sharon M. Hesselbrock ◽  
Linda E. Watson ◽  
Susan R. Barnum

The heterocystous cyanobacteria are currently placed in subsections IV and V, which are distinguished by cellular division in one plane (false branching) and in more than one plane (true branching), respectively. Published phylogenies of 16S rRNA gene sequence data support the monophyly of the heterocystous cyanobacteria, with members of subsection V embedded within subsection IV. It has been postulated that members of subsection V arose from within subsection IV. Therefore, phylogenetic analysis of nucleotide sequences of the nitrogen-fixation gene nifD from representatives of subsections IV and V was performed by using maximum-likelihood criteria. The heterocystous cyanobacteria are supported as being monophyletic, with the non-heterocystous cyanobacteria as their closest relative. However, neither subsection IV nor subsection V is monophyletic, with representatives of both subsections intermixed in two sister clades. Analysis of nifD does not support recognition of two distinct subsections.


2007 ◽  
Vol 43 (1) ◽  
pp. 344-352 ◽  
Author(s):  
N.E. Redmond ◽  
R.W.M. van Soest ◽  
M. Kelly ◽  
J. Raleigh ◽  
S.A.A. Travers ◽  
...  

2006 ◽  
Vol 56 (3) ◽  
pp. 671-673 ◽  
Author(s):  
Paul N. Levett ◽  
Roger E. Morey ◽  
Renee L. Galloway ◽  
Arnold G. Steigerwalt

Isolates of Leptospira from two human cases of leptospirosis in Denmark and France were studied using DNA–DNA relatedness, G+C content, 16S rRNA gene sequence data and pulsed-field gel electrophoresis. These isolates differed from previously described species of Leptospira and are defined as Leptospira broomii sp. nov. The type strain is 5399T (=ATCC BAA-1107T=KIT 5399T).


1993 ◽  
Vol 43 (4) ◽  
pp. 754-760 ◽  
Author(s):  
D. J. SAUL ◽  
A. G. RODRIGO ◽  
R. A. REEVES ◽  
L. C. WILLIAMS ◽  
K. M. BORGES ◽  
...  

Check List ◽  
2021 ◽  
Vol 17 (4) ◽  
pp. 1021-1029
Author(s):  
Hmar Tlawmte Lalremsanga ◽  
Jayaditya Purkayastha ◽  
Mathipi Vabeiryureilai ◽  
Lal Muansanga ◽  
Ht Decemson ◽  
...  

We report a substantial range extension of Ichthyophis multicolor Wilkinson, Presswell, Sherratt, Papadopoulou & Gower, 2014, with new material from Mizoram State, Northeast India. The species was previously known only from its type locality more than 800 km away in Ayeyarwady Region, Myanmar. The species was identified by both its morphology and 16s rRNA gene sequence data. One of the studied individuals represents the largest known specimen for the species (total length = 501 mm; mid-body width = 18.8 mm). Brief comparisons of I. multicolor with the sympatric as well as parapatric congeners in the region, and first barcode data for I. moustakius Kamei, Wilkinson, Gower & Biju, 2009 are also presented.


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