scholarly journals Involvement of the miR156/SPL module in flooding response in Medicago sativa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Biruk A. Feyissa ◽  
Lisa Amyot ◽  
Vida Nasrollahi ◽  
Yousef Papadopoulos ◽  
Susanne E. Kohalmi ◽  
...  
Keyword(s):  
Stress Response ◽  
Medicago Sativa ◽  
Flooding Tolerance ◽  
Adaptive Responses ◽  
Abiotic Stress Response ◽  
Squamosa Promoter Binding Protein ◽  
Transgenic Lines ◽  
Physiological Analysis ◽  
Aba Insensitive

AbstractThe highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription factors. Here we investigated the involvement and possible mechanism of action of the miR156/SPL module in flooding tolerance in alfalfa. For that, we used miR156 overexpressing, SPL13RNAi, flood-tolerant (AAC-Trueman) and -sensitive (AC-Caribou) alfalfa cultivars exposed to flooding. We also used Arabidopsis ABA insensitive (abi1-2, abi5-8) mutants and transgenic lines with either overexpressed (KIN10-OX1, KIN10-OX2) or silenced (KIN10RNAi-1, KIN10RNAi-2) catalytic subunit of SnRK1 to investigate a possible role of ABA and SnRK1 in regulating miR156 expression under flooding. Physiological analysis, hormone profiling and global transcriptome changes revealed a role for miR156/SPL module in flooding tolerance. We also identified nine novel alfalfa SPLs (SPL1, SPL1a, SPL2a, SPL7, SPL7a, SPL8, SPL13a, SPL14, SPL16) responsive to flooding. Our results also showed a possible ABA-dependent SnRK1 upregulation to enhance miR156 expression, resulting in downregulation of SPL4, SPL7a, SPL8, SPL9, SPL13, and SPL13a. We conclude that these effects induce flooding adaptive responses in alfalfa and modulate stress physiology by affecting the transcriptome, ABA metabolites and secondary metabolism.

The role of microRNA in abiotic stress response in plants

2016 ◽  
Vol 50 (3) ◽  
pp. 337-343 ◽  
Author(s):  
N. V. Koroban ◽  
A. V. Kudryavtseva ◽  
G. S. Krasnov ◽  
A. F. Sadritdinova ◽  
M. S. Fedorova ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response

scholarly journals The Role of Micro‐Ribonucleic Acids in Legumes with a Focus on Abiotic Stress Response

2013 ◽  
Vol 6 (3) ◽  
Author(s):  
Nitin Mantri ◽  
Nagalingam Basker ◽  
Rebecca Ford ◽  
Edwin Pang ◽  
Varsha Pardeshi
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response ◽  
Ribonucleic Acids

scholarly journals Positive role of a wheatHvABI5ortholog in abiotic stress response of seedlings

2008 ◽  
Vol 134 (1) ◽  
pp. 74-86 ◽  
Author(s):  
Fuminori Kobayashi ◽  
Eri Maeta ◽  
Akihiro Terashima ◽  
Shigeo Takumi
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response ◽  
Positive Role

scholarly journals Unraveling the role of tomato Bcl-2-associated athanogene (BAG) proteins during abiotic stress response and fruit ripening

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Irfan ◽  
Pankaj Kumar ◽  
Irshad Ahmad ◽  
Asis Datta
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Subcellular Localization ◽  
Plant Development ◽  
Fruit Ripening ◽  
Interaction Network ◽  
In Planta ◽  
Abiotic Stress Response ◽  
Specific Expression

AbstractB-cell lymphoma2 (Bcl-2)-associated athanogene (BAG) family proteins are evolutionary conserved across all eukaryotes. These proteins interact with HSP70/HSC70 and function as co-chaperones during stress response and developmental pathways. Compared to the animal counterpart, the BAG proteins in plants are much less studied and primarily Arabidopsis BAG proteins have been identified and characterized for their role in programmed cell death, homeostasis, growth and development, abiotic and biotic stress response. Here, we have identified BAG protein family (SlBAGs) in tomato, an economically important and a model fruit crop using genome-wide scanning. We have performed phylogenetic analysis, genes architecture assessment, chromosomal location and in silico promoter analysis. Our data suggest that SlBAGs show differential tissue specific expression pattern during plant development particularly fruit development and ripening. Furthermore, we reported that expression of SlBAGs is modulated during abiotic stresses and is regulated by stress hormones ABA and ethylene. In planta subcellular localization reveals their diverse subcellular localization, and many members are localized in nucleus and cytoplasm. Like previous reports, our protein–protein interaction network and yeast two-hybrid analysis uncover that SlBAGs interact with HSP70. The current study provides insights into role of SlBAGs in plant development particualry fruit ripening and abiotic stress response.

The role of receptor-like protein kinases (RLKs) in abiotic stress response in plants

2016 ◽  
Vol 36 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Yaoyao Ye ◽  
Yanfei Ding ◽  
Qiong Jiang ◽  
Feijuan Wang ◽  
Junwei Sun ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Protein Kinases ◽  
Abiotic Stress Response

Emerging concept for the role of photorespiration as an important part of abiotic stress response

Plant Biology ◽  
2013 ◽  
Vol 15 (4) ◽  
pp. 713-722 ◽  
Author(s):  
I. Voss ◽  
B. Sunil ◽  
R. Scheibe ◽  
A. S. Raghavendra
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response

Role of the rice transcription factor JAmyb in abiotic stress response

2012 ◽  
Vol 126 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Naoki Yokotani ◽  
Takanari Ichikawa ◽  
Youichi Kondou ◽  
Masaki Iwabuchi ◽  
Minami Matsui ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response

scholarly journals Jasmonates-Mediated Rewiring of Central Metabolism Regulates Adaptive Responses

2019 ◽  
Vol 60 (12) ◽  
pp. 2613-2620 ◽  
Author(s):  
Tatyana V Savchenko ◽  
Hardy Rolletschek ◽  
Katayoon Dehesh
Keyword(s):  
Central Metabolism ◽  
Adaptive Responses ◽  
Active Growth ◽  
Environmental Challenges ◽  
Collective Data ◽  
Specific Distribution ◽  
Wide Range ◽  
Transgenic Lines ◽  
Branched Chain

Abstract The lipid-derived hormones jasmonates (JAs) play key functions in a wide range of physiological and developmental processes that regulate growth, secondary metabolism and defense against biotic and abiotic stresses. In this connection, biosynthesis, tissue-specific distribution, metabolism, perception, signaling of JAs have been the target of extensive studies. In recent years, the involvement of JAs signaling pathway in the regulation of growth and adaptive responses to environmental challenges has been further examined. However, JAs-mediated mechanisms underlying the transition from ‘growth mode’ to ‘adaptive mode’ remain ambiguous. Combined analysis of transgenic lines deficient in JAs signaling in conjunction with the data from JAs-treated plants revealed the function of these hormones in rewiring of central metabolism. The collective data illustrate JAs-mediated decrease in the levels of metabolites associated with active growth such as sucrose, raffinose, orotate, citrate, malate, and an increase in phosphorylated hexoses, responsible for the suppression of growth and photosynthesis, concurrent with the induction of protective metabolites, such as aromatic and branched-chain amino acids, and aspartate family of metabolites. This finding provides an insight into the function of JAs in shifting the central metabolism from the production of growth-promoting metabolites to protective compounds and expands our understanding of the role of JAs in resource allocation in response to environmental challenges.

scholarly journals BrPP5.2 Overexpression Confers Heat Shock Tolerance in Transgenic Brassica rapa through Inherent Chaperone Activity, Induced Glucosinolate Biosynthesis, and Differential Regulation of Abiotic Stress Response Genes

2021 ◽  
Vol 22 (12) ◽  
pp. 6437
Author(s):  
Muthusamy Muthusamy ◽  
Jonghee Kim ◽  
Sukhee Kim ◽  
Soyoung Park ◽  
Sooin Lee
Keyword(s):  
Heat Stress ◽  
Abiotic Stress ◽  
Stress Response ◽  
Heat Shock ◽  
Brassica Rapa ◽  
Stress Responses ◽  
Abiotic Stress Response ◽  
Glucosinolate Biosynthesis ◽  
Stress Response Genes ◽  
Transgenic Lines

Plant phosphoprotein phosphatases are ubiquitous and multifarious enzymes that respond to developmental requirements and stress signals through reversible dephosphorylation of target proteins. In this study, we investigated the hitherto unknown functions of Brassica rapa protein phosphatase 5.2 (BrPP5.2) by transgenic overexpression of B. rapa lines. The overexpression of BrPP5.2 in transgenic lines conferred heat shock tolerance in 65–89% of the young transgenic seedlings exposed to 46 °C for 25 min. The examination of purified recombinant BrPP5.2 at different molar ratios efficiently prevented the thermal aggregation of malate dehydrogenase at 42 °C, thus suggesting that BrPP5.2 has inherent chaperone activities. The transcriptomic dynamics of transgenic lines, as determined using RNA-seq, revealed that 997 and 1206 (FDR < 0.05, logFC ≥ 2) genes were up- and down-regulated, as compared to non-transgenic controls. Statistical enrichment analyses revealed abiotic stress response genes, including heat stress response (HSR), showed reduced expression in transgenic lines under optimal growth conditions. However, most of the HSR DEGs were upregulated under high temperature stress (37 °C/1 h) conditions. In addition, the glucosinolate biosynthesis gene expression and total glucosinolate content increased in the transgenic lines. These findings provide a new avenue related to BrPP5.2 downstream genes and their crucial metabolic and heat stress responses in plants.

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