scholarly journals Comparison of gene expression in the red imported fire ant (Solenopsis invicta) under different temperature conditions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Vatanparast ◽  
Robert T. Puckett ◽  
Deuk-Soo Choi ◽  
Youngjin Park
Keyword(s):  
Gene Expression ◽  
Gene Ontology ◽  
High Temperature ◽  
Temperature Stress ◽  
Solenopsis Invicta ◽  
Expression Profiles ◽  
Fire Ant ◽  
Molecular Response ◽  
Red Imported Fire Ant ◽  
Imported Fire Ant

AbstractThe red imported fire ant (RIFA), Solenopsis invicta Buren is native to South America and is known as a global problematic invasive species. This study focused on the molecular response of RIFA by comparing gene expression profiles after exposing ants to low (10 °C) and high (40 °C) temperature stress and comparing them to untreated controls (30 °C). A total of 99,085 unigenes (the clustered non-redundant transcripts that are filtered from the longest assembled contigs) were obtained, of which 19,154 were annotated with gene descriptions, gene ontology terms, and metabolic pathways. 86 gene ontology (GO) functional sub-groups and 23 EggNOG terms resulted. Differentially expressed genes (DEGs) with log2FC ≥ 10 were screened and were compared at different temperatures. We found 203, 48, and 66 specific DEGs co-regulated at 10, 20, and 40 °C. Comparing transcriptome profiles for differential gene expression resulted in various DE genes, including cytochrome P450, NADH dehydrogenase subunit 1, cuticle protein and heat shock protein (HSP), which have previously been reported to be involved in cold and high temperature resistance. GO analysis revealed that antioxidant activity is up-regulated under high temperature stress. We verified the RNA-seq data by qPCR on 20 up- and down-regulated DEGs. These findings provide a basis for future understanding of the adaptation mechanisms of RIFA and the molecular mechanisms underlying the response to low and high temperatures.

scholarly journals Comparison of Gene Expression in the Red Imported Fire Ant, Solenopsis Invicta, in Different Temperature Conditions

2021 ◽  
Author(s):  
Youngjin Park ◽  
Mohammad Vatanparast ◽  
Robert Puckett ◽  
Deuk-Soo Choi
Keyword(s):  
Gene Expression ◽  
Gene Ontology ◽  
High Temperature ◽  
Temperature Stress ◽  
Solenopsis Invicta ◽  
Expression Profiles ◽  
Fire Ant ◽  
Molecular Response ◽  
Red Imported Fire Ant ◽  
Imported Fire Ant

Abstract The red imported fire ant (RIFA), Solenopsis invicta Buren is native to South America and is known as a global problematic invasive species. This study focused on the molecular response of RIFA by comparing gene expression profiles after exposing ants to low (10℃) and high (40℃) temperature stress and comparing to untreated controls (30℃). A total of 99,085 unigenes were obtained, of which 19,154 were annotated with gene descriptions, gene ontology terms, and metabolic pathways. 86 gene ontology (GO) functional sub-groups and 23 EggNOG terms resulted. Differentially expressed genes (DEGs) with log2FC ≥ 10 were screened and were compared at different temperatures. We found 203, 48, and 66 specific DEGs co-regulated at 10, 20, and 40℃. Comparing transcriptome profiles for differential gene expression resulted in various DE proteins and genes, including cytochrome P450, NADH dehydrogenase subunit 1, cuticle protein and heat shock protein (HSP) which have previously been reported to be involved in cold and high temperature resistance. GO analysis revealed that antioxidant activity up-regulated under high temperature stress. We verified the RNA-seq data by qPCR on 20 up and down-regulated DEGs. These finding provide a basis for the future understanding of adaptation mechanism of RIFA and molecular mechanism underlying the response to low and high temperatures.

Arginine kinase: Differentiation of gene expression and protein activity in the red imported fire ant, Solenopsis invicta

Gene ◽  
2009 ◽  
Vol 430 (1-2) ◽  
pp. 38-43 ◽  
Author(s):  
Haichuan Wang ◽  
Lan Zhang ◽  
Lee Zhang ◽  
Qin Lin ◽  
Nannan Liu
Keyword(s):  
Gene Expression ◽  
Solenopsis Invicta ◽  
Arginine Kinase ◽  
Fire Ant ◽  
Red Imported Fire Ant ◽  
Protein Activity ◽  
Imported Fire Ant

scholarly journals Altered Expression of Chemosensory and Odorant Binding Proteins in Response to Fungal Infection in the Red Imported Fire Ant, Solenopsis invicta

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhang Wei ◽  
Almudena Ortiz-Urquiza ◽  
Nemat O. Keyhani
Keyword(s):  
Gene Expression ◽  
Fungal Infection ◽  
Post Infection ◽  
Solenopsis Invicta ◽  
Microbial Pathogens ◽  
Fire Ant ◽  
Red Imported Fire Ant ◽  
Odorant Binding Proteins ◽  
Odorant Binding ◽  
Imported Fire Ant

Social insects have evolved acute mechanisms for sensing and mitigating the spread of microbial pathogens within their communities that include complex behaviors such as grooming and sanitation. Chemical sensing involves detection and transport of olfactory and other chemicals that are mediated by at least two distinct classes of small molecular weight soluble proteins known as chemosensory- and odorant binding proteins (CSPs and OBPs, respectively) that exist as protein families in all insects. However, to date, a systematic examination of the expression of these genes involved in olfactory and other pathways to microbial infection has yet to be reported. The red imported fire ant, Solenopsis invicta, is one of the most successful invasive organisms on our planet. Here, we examined the temporal gene expression profiles of a suite of S. invicta CSPs (SiCSPs1-22) and OBPs (SiOBPs1-16) in response to infection by the broad host range fungal insect pathogen, Beauveria bassiana. Our data show that within 24 h post-infection, i.e., before the fungus has penetrated the host cuticle, the expression of SiCSPs and SiOBPs is altered (mainly increased compared to uninfected controls), followed by suppression of SiCSP and select SiOBP expression 48 h post-infection and mixed responses at 72 h post-infection. A smaller group of SiBOPs, however, appeared to respond to fungal infection, with expression of SiOBP15 consistently higher during fungal infection over the time course examined. These data indicate dynamic gene expression responses of CSPs and OBPs to fungal infection that provide clues to mechanisms that might mediate detection of microbial pathogens, triggering grooming, and nest sanitation.

scholarly journals Acute toxicity of typical ant control agents to the red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae)

2021 ◽  
Vol 56 (2) ◽  
pp. 217-224
Author(s):  
Hironori Sakamoto ◽  
Koichi Goka
Keyword(s):  
Acute Toxicity ◽  
Chemical Control ◽  
Solenopsis Invicta ◽  
Fire Ant ◽  
Red Imported Fire Ant ◽  
The Pacific ◽  
New Type ◽  
Ant Control ◽  
Abnormal Behaviors ◽  
Imported Fire Ant

AbstractThe red imported fire ant Solenopsis invicta Buren (Hymenoptera: Formicidae), is a serious invasive alien ant around the world and has expanded its invasive range to the Pacific Rim since the early 2000s. It was first reported in Japan in 2017, and its entry through cargo has been reported numerous times in many ports. Colonies have been found in Tokyo Port since 2019, and now it is an urgent issue to prevent further invasion and establishment. Chemical control is the best tested method of insect control, but we have little information on the efficacy of insecticides against S. invicta in Japan. Here, we conducted acute toxicity assays of six quick-acting pyrethroids (transfluthrin, prallethrin, phenothrin, permethrin, metofluthrin, and pyrethrin) for killing adults and five new-type insecticides (fipronil, thiamethoxam, indoxacarb, imidacloprid, and hydramethylnon) for controlling colonies with toxic baits. We found that the LD50 from six pyrethroids were comparable to each other. The ED50 causing abnormal behaviors were smaller than LD50, but some ants recovered from paralysis within 12 h. Fipronil showed the lowest LD50 suggesting this chemical is the most promising agent for controlling S. invicta. Our results promise to develop a method for the chemical control of S. invicta.

Efficacy of Beauveria bassiana against the red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae), in Taiwan

2011 ◽  
Vol 67 (11) ◽  
pp. 1434-1438 ◽  
Author(s):  
Lekhnath Kafle ◽  
Wen-Jer Wu ◽  
Suey-Sheng Kao ◽  
Cheng-Jen Shih
Keyword(s):  
Beauveria Bassiana ◽  
Solenopsis Invicta ◽  
Fire Ant ◽  
Red Imported Fire Ant ◽  
Imported Fire Ant
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