scholarly journals Biochemical characterization and inhibition of the alternative oxidase enzyme from the fungal phytopathogen Moniliophthora perniciosa

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Mario R. O. Barsottini ◽  
Alice Copsey ◽  
Luke Young ◽  
Renata M. Baroni ◽  
Artur T. Cordeiro ◽  
...  
Keyword(s):  
Alternative Oxidase ◽  
Biochemical Characterization ◽  
Moniliophthora Perniciosa ◽  
Oxidase Enzyme ◽  
Fungal Phytopathogen

Antifungal properties exhibited by bacteria isolated from agriculturally cultivable soils and their antagonistic nature towards fungal phytopathogen suppression

2016 ◽  
Vol 35 (1) ◽  
Author(s):  
Ch. Usha Rani ◽  
Priyanka ◽  
A. Srinivasa Rao
Keyword(s):  
Bacillus Cereus ◽  
Biochemical Characterization ◽  
Rhizosphere Soil ◽  
Agar Medium ◽  
Chitinolytic Activity ◽  
Culture Methods ◽  
Bacterial Cultures ◽  
Nutrient Agar Medium ◽  
Fungal Antagonism ◽  
Fungal Phytopathogen

Soil samples collected from rhizosphere soil, cultivable field soil, Rhizoplane soil of paddy plants and were used to isolate bacteria by culture methods of which thirty two bacterial cultures were identified with diverse colony characteristics on nutrient agar medium and biochemical characterization. Sixteen bacterial isolates were selected for screening fungal antagonism on PDA and were further tested against <italic>Fusarium</italic> and <italic>Rhizoctonia species.</italic> In this study, two <italic>Pseudomonas strains</italic> (RB15, RB30) and <italic>Bacillus cereus</italic> (RB13) were cyanogen producers. Both strains of <italic>Pseudomonas</italic> RB15 and RB30 and <italic>Bacillus cereus</italic> (RB13) were also siderophore producers. Only three isolates showed chitinolytic activity; <italic>Serratia marcesens (RB24), Bacillus cereus (RB13) and Enterobacter cancerogenus</italic> (RB17).

scholarly journals The hemibiotrophic cacao pathogen Moniliophthora perniciosa depends on a mitochondrial alternative oxidase for biotrophic development

2012 ◽  
Vol 194 (4) ◽  
pp. 1025-1034 ◽  
Author(s):  
Daniela P. T. Thomazella ◽  
Paulo José P. L. Teixeira ◽  
Halley C. Oliveira ◽  
Elzira E. Saviani ◽  
Johana Rincones ◽  
...  
Keyword(s):  
Alternative Oxidase ◽  
Moniliophthora Perniciosa

Probing mammalian spermine oxidase enzyme–substrate complex through molecular modeling, site-directed mutagenesis and biochemical characterization

Amino Acids ◽  
2010 ◽  
Vol 40 (4) ◽  
pp. 1115-1126 ◽  
Author(s):  
Paraskevi Tavladoraki ◽  
Manuela Cervelli ◽  
Fabrizio Antonangeli ◽  
Giovanni Minervini ◽  
Pasquale Stano ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Biochemical Characterization ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Spermine Oxidase ◽  
Substrate Complex ◽  
Enzyme Substrate ◽  
Oxidase Enzyme

scholarly journals Self-assembled proteolipossomes to functionally characterize the alternative oxidase from Moniliophthora perniciosa

2018 ◽  
Vol 1859 ◽  
pp. e65-e66
Author(s):  
Mario R.O. Barsottini ◽  
Alice Copsey ◽  
Fei Xu ◽  
Gonçalo A.G. Pereira ◽  
Anthony L. Moore
Keyword(s):  
Alternative Oxidase ◽  
Moniliophthora Perniciosa ◽  
Self Assembled

scholarly journals Functional characterization of potential antifungal agents based on molecules derived from diphenylamides on Moniliphthora perniciosa, etiological agent of cacao Witches' Broom Disease

2018 ◽  
Author(s):  
Bárbara Aliende Pires ◽  
Goncalo Amarante Guimaraes Pereira ◽  
Mario Ramos de Oliveira Barsottini
Keyword(s):  
Culture Medium ◽  
Alternative Oxidase ◽  
Antifungal Agents ◽  
Effective Means ◽  
Functional Characterization ◽  
Moniliophthora Perniciosa ◽  
Liquid Culture Medium ◽  
Broom Disease

Moniliophthora perniciosa is a fungal pathogen and causal agent of Witches' Broom Disease of cocoa (Theobroma cacao), which generates large losses for the cocoa culture. Currently, there is no effective means to control this disease, but the enzyme Alternative Oxidase (AOX) is a promissing target for the development of novel fungicides against M. perniciosa. Here, our aim is to develop a methodology to evaluate the antifungal activity of AOX inhibitors in vitro through growth measurements of M. perniciosa in liquid culture medium.

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

The in vivo distribution and dynamics of DNA topoisomerase II in Drosophila embryonic nuclei and chromosomes

1993 ◽  
Vol 51 ◽  
pp. 74-75
Author(s):  
Jason R. Swedlow ◽  
Neil Osheroff ◽  
Tim Karr ◽  
John W. Sedat ◽  
David A. Agard
Keyword(s):  
Topoisomerase Ii ◽  
Biochemical Characterization ◽  
Developmental Cycle ◽  
Dna Topoisomerase Ii ◽  
Chromosomal Distribution ◽  
Dna Topoisomerase ◽  
Ideal System ◽  
Dnase Treatment

DNA topoisomerase II is an ATP-dependent double-stranded DNA strand-passing enzyme that is necessary for full condensation of chromosomes and for complete segregation of sister chromatids at mitosis in vivo and in vitro. Biochemical characterization of chromosomes or nuclei after extraction with high-salt or detergents and DNAse treatment showed that topoisomerase II was a major component of this remnant, termed the chromosome scaffold. The scaffold has been hypothesized to be the structural backbone of the chromosome, so the localization of topoisomerase II to die scaffold suggested that the enzyme might play a structural role in the chromosome. However, topoisomerase II has not been studied in nuclei or chromosomes in vivo. We have monitored the chromosomal distribution of topoisomerase II in vivo during mitosis in the Drosophila embryo. This embryo forms a multi-nucleated syncytial blastoderm early in its developmental cycle. During this time, the embryonic nuclei synchronously progress through 13 mitotic cycles, so this is an ideal system to follow nuclear and chromosomal dynamics.

Sign in / Sign up

Export Citation Format

Share Document