scholarly journals Selection of Reference Genes for Gene Expression Studies related to lung injury in a preterm lamb model

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Prue M. Pereira-Fantini ◽  
Anushi E. Rajapaksa ◽  
Regina Oakley ◽  
David G. Tingay
Keyword(s):  
Gene Expression ◽  
Lung Injury ◽  
Reference Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Selection of reference genes for measuring the expression of aiiO in Ochrobactrum quorumnocens A44 using RT-qPCR

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dorota M. Krzyżanowska ◽  
Anna Supernat ◽  
Tomasz Maciąg ◽  
Marta Matuszewska ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Reference Genes ◽  
Significance Threshold ◽  
Expression Studies ◽  
Reverse Transcription Quantitative Pcr ◽  
Gene Expression Studies ◽  
The Stability ◽  
Selection Of

Abstract Reverse transcription quantitative PCR (RT-qPCR), a method of choice for quantification of gene expression changes, requires stably expressed reference genes for normalization of data. So far, no reference genes were established for the Alphaproteobacteria of the genus Ochrobactrum. Here, we determined reference genes for gene expression studies in O. quorumnocens A44. Strain A44 was cultured under 10 different conditions and the stability of expression of 11 candidate genes was evaluated using geNorm, NormFinder and BestKeeper. Most stably expressed genes were found to be rho, gyrB and rpoD. Our results can facilitate the choice of reference genes in the related Ochrobactrum strains. O. quorumnocens A44 is able to inactivate a broad spectrum of N-acyl homoserine lactones (AHLs) – the quorum sensing molecules of many Gram-negative bacteria. This activity is attributed to AiiO hydrolase, yet it remains unclear whether AHLs are the primary substrate of this enzyme. Using the established RT-qPCR setup, we found that the expression of the aiiO gene upon exposure to two AHLs, C6-HLS and 3OC12-HSL, does not change above the 1-fold significance threshold. The implications of this finding are discussed in the light of the role of quorum sensing-interfering enzymes in the host strains.

Selection of optimal reference genes for gene expression studies in chronically hypoxic rat heart

2019 ◽  
Vol 461 (1-2) ◽  
pp. 15-22 ◽  
Author(s):  
Daniel Benak ◽  
Dita Sotakova-Kasparova ◽  
Jan Neckar ◽  
Frantisek Kolar ◽  
Marketa Hlavackova
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Rat Heart ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz

2012 ◽  
Vol 138 ◽  
pp. 128-133 ◽  
Author(s):  
Shanjun Yi ◽  
Yongqiang Qian ◽  
Lei Han ◽  
Zhenyuan Sun ◽  
Chengming Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of
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