scholarly journals Untargeted metabolomics of colonic digests reveals kynurenine pathway metabolites, dityrosine and 3-dehydroxycarnitine as red versus white meat discriminating metabolites

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Caroline Rombouts ◽  
Lieselot Y. Hemeryck ◽  
Thomas Van Hecke ◽  
Stefaan De Smet ◽  
Winnok H. De Vos ◽  
...  
Metabolomics ◽  
2019 ◽  
Vol 15 (11) ◽  
Author(s):  
Manuela Simonato ◽  
Igor Fochi ◽  
Luca Vedovelli ◽  
Sonia Giambelluca ◽  
Cristiana Carollo ◽  
...  

Abstract Introduction Transposition of the great arteries (TGA) is a cyanotic congenital heart defect that requires surgical correction, with the use of cardiopulmonary-bypass (CPB), usually within 3 weeks of life. The use of CPB in open heart surgery results in brain hypoperfusion and in a powerful systemic inflammatory response and oxidative stress. Objective We aimed to develop a novel untargeted metabolomics approach to detect early postoperative changes in metabolic profile following neonatal cardiac surgery. Methods We studied 14 TGA newborns with intact ventricular septum undergoing arterial switch operation with the use of CPB. Urine samples were collected preoperatively and at the end of the surgery and were analyzed using an untargeted metabolomics approach based on UHPLC-high resolution mass spectrometry. Results Since post surgery metabolic spectra were heavily contaminated by metabolites derived from administered drugs, we constructed a list of drugs used during surgery and their related metabolites retrieved from urine samples. This library was applied to our samples and 1255 drugs and drug metabolites were excluded from the analysis. Afterward, we detected over 39,000 unique compounds and 371 putatively annotated metabolites were different between pre and post-surgery samples. Among these metabolites, 13 were correctly annotated or identified. Metabolites linked to kynurenine pathway of tryptophan degradation displayed the highest fold change. Conclusions This is the first report on metabolic response to cardiac surgery in TGA newborns. We developed an experimental design that allowed the identification of perturbed metabolic pathways and potential biomarkers of brain damage, limiting drugs interference in the analysis.


2020 ◽  
Vol 64 (12) ◽  
pp. 2000070
Author(s):  
Sophie Goethals ◽  
Caroline Rombouts ◽  
Lieselot Y. Hemeryck ◽  
Lieven Van Meulebroek ◽  
Thomas Van Hecke ◽  
...  

2021 ◽  
pp. 2000463
Author(s):  
Caroline Rombouts ◽  
Lieven Van Meulebroek ◽  
Margot De Spiegeleer ◽  
Sophie Goethals ◽  
Thomas Van Hecke ◽  
...  

2018 ◽  
Vol 1 (01) ◽  
pp. 18-21
Author(s):  
Yulian Syahputri ◽  
Diana Widiastuti

Dragon fruit skin, waste material (dragon fruit waste), will have a profitable sale value if it can be used as a food raw material. Dragon fruit skin waste contains relatively high dietary fiber so it can be used as a food raw material. This study aims to utilize dragon fruit skin waste for the manufacture of dragon fruit skin flour as an alternative food source. Some tests are made on white-meat dragon fruit skin, red-meat dragon fruit skin and super red dragon fruit skin. The preliminary study is the soaking of the three types of dragon fruit skin in two solutions, namely 0.1% sodium citrate and 0.1% sodium metabisulfite to prevent the browning effect on flour. Dragon fruit skin flour from the soaking with both solutions is then characterized physically, including its texture, color, flavor and rendement. The best physical characterization is followed by chemical characterization, including the contents of water, ash, protein, fat, carbohydrate, dietary fiber, minerals (Fe, Na, K, Ca and P), and also microbiological characterization of Escherichia coli, molds and Bacillus cereus. The chemical and microbiological characterization shows that the red-meat dragon fruit skin flour has better results than the white-meat and super red dragon fruit skin flour does. The red-meat dragon fruit skin flour contains 8.80% water, 0.20% ash, 2.35% fat, 7.69%, protein, 68,29% carbohydrate and 28,72% dietary fiber as well as 4.40 mg K, 8.76 mg Na , 0.65 mg Fe , 10.20 mg Ca and 32.58 mg P. Keywords: Waste, Dragon Fruit Skin, Dragon Fruit Skin Powder, Alternative Food Source


Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 2041-P
Author(s):  
TAKASHI YAMAMOTO ◽  
TAKANARI GOTODA

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