untargeted metabolomics
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2022 ◽  
Vol 374 ◽  
pp. 131740
Caio de Oliveira Gorgulho Silva ◽  
Joice Raisa Barbosa Cunha ◽  
Aparecido Almeida Conceição ◽  
Euziclei Gonzaga Almeida ◽  
Diego Cunha Zied ◽  

2022 ◽  
Vol 807 ◽  
pp. 150717
Mélina Ramos ◽  
Hikmat Ghosson ◽  
Delphine Raviglione ◽  
Cédric Bertrand ◽  
Marie-Virginie Salvia

2022 ◽  
Vol 176 ◽  
pp. 114411
Chao-Ran Li ◽  
Liu-Xiu Yang ◽  
Zi-Fan Guo ◽  
Hua Yang ◽  
Ying Zhang ◽  

2022 ◽  
Vol 12 ◽  
Xu Zeng ◽  
Jiaxue Li ◽  
Xinkai Lyu ◽  
Juan Chen ◽  
Xiaomei Chen ◽  

Codonopsis pilosula has been used in traditional Chinese medicine for hundreds of years, where it has been used to treat anaemia, fatigue, a weak spleen, and stomach problems, among other ailments. The roots of C. pilosula are considered medicinal, while the aerial parts are always directly discarded after harvest in autumn or winter. Some studies have shown that the stems and leaves of C. pilosula also contain a variety of active metabolites, including saponins, flavonoids, terpenoids, and polysaccharides. To efficiently utilise resources, waste products from C. pilosula leaves and stems were analysed by untargeted metabolomics and chemometrics. A total of 1508 metabolites were detected and annotated, of which 463 were identified as differentially expressed metabolites (DEMs). These DEMs were grouped into classes, such as carboxylic acids and derivatives, steroids, organic oxygen compounds, fatty acyls, prenol lipids, and flavonoids. Metabolic profiling of C. pilosula tissues showed that the contents of polyacetylenes, polyenes, flavonoids, some alkaloids, steroids, terpenoids, and organic acids were higher in stems and leaves, whereas the contents of the main lignans and some alkaloids were more enriched in roots. Moreover, C. pilosula stems and leaves also contained a lobetyolin, syringin and atractylenolide III, which were detected by LC-MS/MS and HPLC-UV. The extracts of C. pilosula aerial parts also showed stronger antioxidant properties than roots. C. pilosula stems and leaves were rich in active ingredients and might have great value for development and utilisation.

Nutrients ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 214
Lukasz Szczerbinski ◽  
Gladys Wojciechowska ◽  
Adam Olichwier ◽  
Mark A. Taylor ◽  
Urszula Puchta ◽  

Obesity rates among children are growing rapidly worldwide, placing massive pressure on healthcare systems. Untargeted metabolomics can expand our understanding of the pathogenesis of obesity and elucidate mechanisms related to its symptoms. However, the metabolic signatures of obesity in children have not been thoroughly investigated. Herein, we explored metabolites associated with obesity development in childhood. Untargeted metabolomic profiling was performed on fasting serum samples from 27 obese Caucasian children and adolescents and 15 sex- and age-matched normal-weight children. Three metabolomic assays were combined and yielded 726 unique identified metabolites: gas chromatography–mass spectrometry (GC–MS), hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC LC–MS/MS), and lipidomics. Univariate and multivariate analyses showed clear discrimination between the untargeted metabolomes of obese and normal-weight children, with 162 significantly differentially expressed metabolites between groups. Children with obesity had higher concentrations of branch-chained amino acids and various lipid metabolites, including phosphatidylcholines, cholesteryl esters, triglycerides. Thus, an early manifestation of obesity pathogenesis and its metabolic consequences in the serum metabolome are correlated with altered lipid metabolism. Obesity metabolite patterns in the adult population were very similar to the metabolic signature of childhood obesity. Identified metabolites could be potential biomarkers and used to study obesity pathomechanisms.

2022 ◽  
pp. 123114
Ke Bao ◽  
Zonghui Jing ◽  
Qian Wang ◽  
Zhiheng Huang ◽  
Dongsheng Han ◽  

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