Formulation of Gliclazide Encapsulated Chitosan Nanoparticles: In Vitro and In Vivo Evaluation

2012 ◽  
pp. 77-85 ◽  
Author(s):  
Ranjith Kumar Averineni ◽  
Gopal Venkatesh Shavi ◽  
Om Prakash Ranjan ◽  
Praful Balavant Deshpande ◽  
Gurram Aravind Kumar ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
In Vivo Evaluation

Simvastatin nanoparticles loaded polymeric film as a potential strategy for diabetic wound healing: in vitro and in vivo evaluation

2021 ◽  
Vol 18 ◽  
Author(s):  
Saima Tufail ◽  
Muhammad Irfan Siddique ◽  
Muhammad Sarfraz ◽  
Muhammad Farhan Sohail ◽  
Muhammad Nabeel Shahid ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Chitosan Nanoparticles ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Diabetic Wound ◽  
In Vivo Evaluation ◽  
Diabetic Wound Healing

Introduction: The pleiotropic effects of statins are recently explored for wound healing through angiogenesis and lymph-angiogenesis that could be of great importance in diabetic wounds. Aim: Aim of the present study is to fabricate nanofilm embedded with simvastatin loaded chitosan nanoparticles (CS-SIM-NPs) has been reported herein to explore the efficacy of SIM in diabetic wound healing. Methods: The NPs, prepared via ionic gelation, were 173nm ± 2.645 in size with a zeta potential -0.299 ± 0.009 and PDI 0.051 ± 0.088 with excellent encapsulation efficiency (99.97%). The optimized formulation (CS: TPP, 1:1) that exhibited the highest drug release (91.64%) was incorporated into polymeric nanofilm (HPMC, Sodium alginate, PVA), followed by in vitro characterization. The optimized nanofilm was applied to the wound created on the back of diabetes-induced (with alloxan injection 120 mg/kg) albino rats. Results: The results showed significant (p < 0.05) improvement in the wound healing process compared to the diabetes-induced non-treated group. The results highlighted the importance of nanofilms loaded with SIM-NPs in diabetic wound healing through angiogenesis promotion at the wound site. Conclusion: Thus, CS-SIM-NPs loaded polymeric nanofilms could be an emerging diabetic wound healing agent in the industry of nanomedicines.

scholarly journals In Vitro and in Vivo Evaluation of Osteoporosis Therapeutic Peptide PTH 1–34 Loaded PEGylated Chitosan Nanoparticles

2013 ◽  
Vol 10 (11) ◽  
pp. 4159-4167 ◽  
Author(s):  
Deepa Narayanan ◽  
A. Anitha ◽  
R. Jayakumar ◽  
K. P. Chennazhi
Keyword(s):  
Chitosan Nanoparticles ◽  
In Vivo Evaluation ◽  
Therapeutic Peptide

scholarly journals A nanoformulation of siRNA and its role in cancer therapy: In vitro and in vivo evaluation

2013 ◽  
Vol 18 (1) ◽  
Author(s):  
Hitesh Jagani ◽  
Josyula Rao ◽  
Vasanth Palanimuthu ◽  
Raghu Hariharapura ◽  
Sagar Gang
Keyword(s):  
Chitosan Nanoparticles ◽  
Cancer Drugs ◽  
Neoplastic Cells ◽  
In Vivo Evaluation ◽  
Anti Cancer ◽  
Effective Delivery ◽  
Interfering Rna ◽  
Cancerous Cells

AbstractOverexpression of anti-apoptotic Bcl-2 is often observed in a wide variety of human cancers. It prevents the induction of apoptosis in neoplastic cells and contributes to resistance to chemotherapy. RNA interference has emerged as an efficient and selective technique for gene silencing. The potential to use small interfering RNA (siRNA) as a therapeutic agent for the treatment of cancer has elicited a great deal of interest. However, insufficient cellular uptake and poor stability have limited its therapeutic applications. The purpose of this study was to prepare chitosan nanoparticles via ionic gelation of chitosan by tripolyphosphate for effective delivery of siRNA to silence the anti-apoptotic Bcl-2 gene in neoplastic cells. Chitosan nanoparticles loaded with siRNA were in the size range 190 to 340 nm with a polydispersive index ranging from 0.04 to 0.2. They were able to completely bind with siRNA, provide protection against nuclease degradation, and enhance the transfection. Cell culture studies revealed that nanoparticles with entrapped siRNA could efficiently silence the antiapoptotic Bcl-2 gene. Studies on Swiss albino mice showed that siRNA could be effectively delivered through nanoparticles. There was significant decrease in the tumor volume. Blocking the expression of anti-apoptotic Bcl-2 can enhance the sensitivity of cancerous cells to anti-cancer drugs and the apoptosis rate. Therefore, nanoformulations with siRNA can be promoted as an adjuvant therapy in combination with anti-cancer drugs.

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