Microarray-guided discovery of two-photon (2P) small molecule probes for live-cell imaging of cysteinyl cathepsin activities

2012 ◽  
Vol 48 (58) ◽  
pp. 7304 ◽  
Author(s):  
Zhenkun Na ◽  
Lin Li ◽  
Mahesh Uttamchandani ◽  
Shao Q. Yao
Keyword(s):  
Small Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Guided Discovery ◽  
Two Photon ◽  
Small Molecule Probes

scholarly journals Live-Cell Imaging of Endogenous mRNAs with a Small Molecule

2014 ◽  
Vol 127 (6) ◽  
pp. 1875-1878 ◽  
Author(s):  
Shin-ichi Sato ◽  
Mizuki Watanabe ◽  
Yousuke Katsuda ◽  
Asako Murata ◽  
Dan Ohtan Wang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

2014 ◽  
Vol 26 (1) ◽  
pp. 25-30
Author(s):  
Yoko Mizuta ◽  
Daisuke Kurihara ◽  
Tetsuya Higashiyama
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Plant Tissues ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

High Speed Two Photon Excitation Microscopy in Live Cell Imaging using Image Correlation Spectroscopy (ICS)

2001 ◽  
Vol 7 (S2) ◽  
pp. 22-23
Author(s):  
P. W. Wiseman ◽  
J. C. Bouwer ◽  
S. Peltier ◽  
M. H. Ellisman
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Live Cell ◽  
Image Correlation ◽  
Scanning System ◽  
Optical Sectioning ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

For live-cell imaging, two-photon excitation microscopy (TPEM) is proving to be a significant technological advancement. The unique features offered by TPEM are the ability to image thick sections, excellent optical sectioning capabilities, low damage to living cells, and less out of focus fluorescence and out of focus photobleaching. of these features, the most useful for the biological microscopist, is optical sectioning. Optical sectioning is an intrinsic property of the two-photon process, whereby, two infrared (IR) photons are absorbed quickly to excite a single UV/blue transition. The probability for exciting a two photon transition is proportional to the instantaneous excitation intensity squared. Therefore, for a focused laser beam, only light at the focal point of the excitation beam excites a fluorescent transition. Thus, the need for confocal apertures and time consuming deconvolution algorithms are, for the most part, eliminated.We have continued to develop and enhance our ability to perform high-speed, two-photon excitation fluorescence microscopy. in 1998, we successfully deployed a prototype, video-rate twophoton laser scanning system (30 frames/sec or faster at reduced scan width) developed with support from Nikon Corporation. That system was built upon a Nikon RCM 8000 confocal microscope.

scholarly journals Two-photon targeted recording of GFP-expressing neurons for light responses and live-cell imaging in the mouse retina

2010 ◽  
Vol 5 (7) ◽  
pp. 1347-1352 ◽  
Author(s):  
Wei Wei ◽  
Justin Elstrott ◽  
Marla B Feller
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Mouse Retina ◽  
Light Responses ◽  
Two Photon

Bionanoprobes with Excellent Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

2010 ◽  
Vol 16 (29) ◽  
pp. 8647-8651 ◽  
Author(s):  
Guangsheng Shao ◽  
Rongcheng Han ◽  
Yan Ma ◽  
Minxian Tang ◽  
Fumin Xue ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Luminescence Properties ◽  
Two Photon

Construction of an orthogonal ZnSalen/Salophen library as a colour palette for one- and two-photon live cell imaging

2014 ◽  
Vol 5 (6) ◽  
pp. 2318 ◽  
Author(s):  
Da Xie ◽  
Jing Jing ◽  
Yuan-Bo Cai ◽  
Juan Tang ◽  
Juan-Juan Chen ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Two Photon ◽  
Colour Palette

scholarly journals Small Molecule-Photoactive Yellow Protein Labeling Technology in Live Cell Imaging

Molecules ◽  
2016 ◽  
Vol 21 (9) ◽  
pp. 1163 ◽  
Author(s):  
Feng Gao ◽  
Tang Gao ◽  
Kechao Zhou ◽  
Wenbin Zeng
Keyword(s):  
Small Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Protein Labeling ◽  
Photoactive Yellow Protein
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