Characterization of the recognition of Candida species by mannose-binding lectin using surface plasmon resonance

The Analyst ◽  
2013 ◽  
Vol 138 (8) ◽  
pp. 2477 ◽  
Author(s):  
Sébastien Damiens ◽  
Pierre Marie Danze ◽  
Anne-Sophie Drucbert ◽  
Laura Choteau ◽  
Thierry Jouault ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Candida Species ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin

scholarly journals A New Surface Plasmon Resonance Assay for In Vitro Screening of Mannose-Binding Lectin Inhibitors

2016 ◽  
Vol 21 (7) ◽  
pp. 749-757 ◽  
Author(s):  
Matteo Stravalaci ◽  
Daiana De Blasio ◽  
Franca Orsini ◽  
Carlo Perego ◽  
Alessandro Palmioli ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Ischemia Reperfusion ◽  
Mannose Binding Lectin ◽  
Lectin Pathway ◽  
In Vitro Screening ◽  
Mannose Binding ◽  
Binding Lectin

Mannose-binding lectin (MBL) is a circulating protein that acts as a soluble pattern recognition molecule of the innate immunity. It binds to carbohydrate patterns on the surface of pathogens or of altered self-cells, with activation of the lectin pathway of the complement system. Recent evidence indicates that MBL contributes to the pathophysiology of ischemia-reperfusion injury and other conditions. Thus, MBL inhibitors offer promising therapeutic strategies, since they prevent the interaction of MBL with its target sugar arrays. We developed and characterized a novel assay based on surface plasmon resonance for in vitro screening of these compounds, which may be useful before the more expensive and time-consuming in vivo studies. The assay measures the inhibitor’s ability to interfere with the binding of murine MBL-A or MBL-C, or of human recombinant MBL, to mannose residues immobilized on the sensor chip surface. We have applied the assay to measure the IC50 of synthetic glycodendrimers, two of them with neuroprotective properties in animal models of MBL-mediated injuries.

scholarly journals Pharmacological inhibition of mannose-binding lectin ameliorates neurobehavioral dysfunction following experimental traumatic brain injury

2016 ◽  
Vol 37 (3) ◽  
pp. 938-950 ◽  
Author(s):  
Daiana De Blasio ◽  
Stefano Fumagalli ◽  
Luca Longhi ◽  
Franca Orsini ◽  
Alessandro Palmioli ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Surface Plasmon Resonance ◽  
Brain Injury ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mannose Binding Lectin ◽  
Brain Injured ◽  
Mannose Binding ◽  
Traumatic Brain Injured ◽  
Binding Lectin

Mannose-binding lectin is present in the contusion area of traumatic brain-injured patients and in that of traumatic brain-injured mice, where mannose-binding lectin-C exceeds mannose-binding lectin-A. The reduced susceptibility to traumatic brain injury of mannose-binding lectin double knock-out mice (mannose-binding lectin−/−) when compared to wild type mice suggests that mannose-binding lectin may be a therapeutic target following traumatic brain injury. Here, we evaluated the effects of a multivalent glycomimetic mannose-binding lectin ligand, Polyman9, following traumatic brain injury in mice. In vitro surface plasmon resonance assay indicated that Polyman9 dose-dependently inhibits the binding to immobilized mannose residues of plasma mannose-binding lectin-C selectively over that of mannose-binding lectin-A. Male C57Bl/6 mice underwent sham/controlled cortical impact traumatic brain injury and intravenous treatment with Polyman9/saline. Ex-vivo surface plasmon resonance studies confirmed that Polyman9 effectively reduces the binding of plasma mannose-binding lectin-C to immobilized mannose residues. In vivo studies up to four weeks post injury, showed that Polyman9 induces significant improvement in sensorimotor deficits (by neuroscore and beam walk), promotes neurogenesis (73% increase in doublecortin immunoreactivity), and astrogliosis (28% increase in glial fibrillary acid protein). Polyman9 administration in brain-injured mannose-binding lectin−/− mice had no effect on post-traumatic brain-injured functional deficits, suggestive of the specificity of its neuroprotective effects. The neurobehavioral efficacy of Polyman9 implicates mannose-binding lectin-C as a novel therapeutic target for traumatic brain injury.

Molecular cloning and characterization of a mannose-binding lectin gene from Crinum asiaticum

2003 ◽  
Vol 160 (8) ◽  
pp. 913-920 ◽  
Author(s):  
Yourong Chai ◽  
Yongzhen Pang ◽  
Zhihua Liao ◽  
L.e.i. Zhang ◽  
Xiaofen Sun ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Mannose Binding Lectin ◽  
Lectin Gene ◽  
Mannose Binding ◽  
Crinum Asiaticum ◽  
Binding Lectin

Characterization of Surface-Confined α-Synuclein by Surface Plasmon Resonance Measurements

Langmuir ◽  
2006 ◽  
Vol 22 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Taewook Kang ◽  
Surin Hong ◽  
Hyun Jin Kim ◽  
Jungwoo Moon ◽  
Seogil Oh ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance

Development and Characterization of a Diamond-Based Localized Surface Plasmon Resonance Interface

2010 ◽  
Vol 114 (8) ◽  
pp. 3346-3353 ◽  
Author(s):  
Sabine Szunerits ◽  
Slimane Ghodbane ◽  
Joanna Niedziółka-Jönsson ◽  
Elisabeth Galopin ◽  
Frederik Klauser ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Localized Surface Plasmon

Characterization of labeled reagents in ligand-binding assays by a surface plasmon resonance biosensor

Bioanalysis ◽  
2017 ◽  
Vol 9 (2) ◽  
pp. 193-207 ◽  
Author(s):  
Jia Duo ◽  
JoAnne Bruno ◽  
Steven Piccoli ◽  
Binodh DeSilva ◽  
Yan J Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assays ◽  
Surface Plasmon Resonance Biosensor

scholarly journals Characterization of thermal bump due to surface plasmon resonance

2019 ◽  
Vol 27 (15) ◽  
pp. 21717
Author(s):  
Tsz Kit Yung ◽  
Ranran Zhang ◽  
Qiuling Zhao ◽  
Xia Wang ◽  
Wensheng Gao ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance
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