Novel surface associated polyphosphate bodies sequester uranium in the filamentous, marine cyanobacterium, Anabaena torulosa

Metallomics ◽  
2013 ◽  
Vol 5 (12) ◽  
pp. 1595 ◽  
Author(s):  
Celin Acharya ◽  
Shree Kumar Apte

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Sarah Pfeffer ◽  
R. Malcolm Brown

This study presents the complete nucleotide sequence of Anabaena sp. ATCC 33047 ( Anabaena CA), a filamentous, nitrogen-fixing marine cyanobacterium, which under salt stress conditions accumulates sucrose internally. The elucidation of the genome will contribute to the understanding of cyanobacterial diversity.



Author(s):  
L. P. Hardie ◽  
D. L. Balkwill ◽  
S. E. Stevens

Agmenellum quadruplicatum is a unicellular, non-nitrogen-fixing, marine cyanobacterium (blue-green alga). The ultrastructure of this organism, when grown in the laboratory with all necessary nutrients, has been characterized thoroughly. In contrast, little is known of its ultrastructure in the specific nutrient-limiting conditions typical of its natural habitat. Iron is one of the nutrients likely to limit this organism in such natural environments. It is also of great importance metabolically, being required for both photosynthesis and assimilation of nitrate. The purpose of this study was to assess the effects (if any) of iron limitation on the ultrastructure of A. quadruplicatum. It was part of a broader endeavor to elucidate the ultrastructure of cyanobacteria in natural systemsActively growing cells were placed in a growth medium containing 1% of its usual iron. The cultures were then sampled periodically for 10 days and prepared for thin sectioning TEM to assess the effects of iron limitation.



Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.



BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (15) ◽  
Author(s):  
Kangming Chen ◽  
Huilan Zhu ◽  
Liping Gu ◽  
Shengni Tian ◽  
Ruanbao Zhou




2015 ◽  
Vol 12 ◽  
pp. 463-469 ◽  
Author(s):  
J.I.S. Khattar ◽  
S. Kaur ◽  
S. Kaushal ◽  
Y. Singh ◽  
D.P. Singh ◽  
...  


Author(s):  
Akira Ebihara ◽  
Arihiro Iwasaki ◽  
Youhei Miura ◽  
Ghulam Jeelani ◽  
Tomoyoshi Nozaki ◽  
...  


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