Recombinant silk fibroin incorporated cell-adhesive sequences produced by transgenic silkworm as a possible candidate for use in vascular graft

2014 ◽  
Vol 2 (42) ◽  
pp. 7375-7383 ◽  
Author(s):  
Tetsuo Asakura ◽  
Makoto Isozaki ◽  
Toshiki Saotome ◽  
Ken-ichiro Tatematsu ◽  
Hideki Sezutsu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Graft ◽  
Transgenic Silkworm ◽  
Cell Adhesive ◽  
Silk Fibroins

Transgenic silk fibroins that incorporated the laminin sequence were prepared. The adhesive activities tend to increase in the TG silk fibroins relative to WT silk fibroins.

Introduction of VEGF or RGD sequences improves revascularization properties of Bombyx mori silk fibroin produced by transgenic silkworm

2015 ◽  
Vol 3 (35) ◽  
pp. 7109-7116 ◽  
Author(s):  
Toshiki Saotome ◽  
Haruki Hayashi ◽  
Ryo Tanaka ◽  
Atsushi Kinugasa ◽  
Shouji Uesugi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Platelet Adhesion ◽  
Transgenic Silkworm ◽  
Silk Fibroins ◽  
Bombyx Mori Silk

Transgenic silk fibroins incorporated the VEGF and RGD were prepared. The VEGF SF showed lower platelet adhesion than the RGD SF and WT SF. An in vivo implantation study supported these in vitro results.

scholarly journals Preparation of Braiding Silk Vascular Graft Coated by Silk Fibroin and Evaluation by Implantation into Dog Abdominal Aorta

2014 ◽  
Vol 70 (12) ◽  
pp. 281-287 ◽  
Author(s):  
Toshiki Saotome ◽  
Shoji Uesugi ◽  
Atsushi Kinugasa ◽  
Satoshi Yamamoto ◽  
Hiroyuki Okamoto ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Abdominal Aorta ◽  
Vascular Graft

scholarly journals Biodegradable Extremely-Small-Diameter Vascular Graft Made of Silk Fibroin can be Implanted in Mice

2020 ◽  
Vol 27 (12) ◽  
pp. 1299-1309 ◽  
Author(s):  
Kimie Tanaka ◽  
Daiju Fukuda ◽  
Yasutomi Higashikuni ◽  
Yoichiro Hirata ◽  
Issei Komuro ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Graft ◽  
Small Diameter

Regeneration of the femoral epicondyle on calcium-binding silk scaffolds developed using transgenic silk fibroin produced by transgenic silkworm

2011 ◽  
Vol 7 (3) ◽  
pp. 1192-1201 ◽  
Author(s):  
Aya Nagano ◽  
Yumi Tanioka ◽  
Nobuko Sakurai ◽  
Hideki Sezutsu ◽  
Noboru Kuboyama ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Calcium Binding ◽  
Transgenic Silkworm ◽  
Silk Scaffolds

scholarly journals Silk Fibroin Vascular Graft: From Design to In Vitro and In Vivo Test

2020 ◽  
Vol 48 ◽  
pp. 55
Author(s):  
Alberto Maria Settembrini ◽  
Antonio Alessandrino ◽  
Anna Chiarini ◽  
Giuliano Freddi ◽  
Ilaria Dal Prà ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Graft ◽  
In Vivo Test

scholarly journals Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Huijing Zhao ◽  
Siyong Xiong ◽  
Mingzhong Li ◽  
Qiang Zhang ◽  
Guiyang Liu
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Gelation Time ◽  
X Ray Diffraction ◽  
Antheraea Pernyi ◽  
X Ray ◽  
Silk Fibroins ◽  
Ft Ir ◽  
Additional Formation ◽  
Ir And Raman

Silk fibroin (SF) hydrogels were obtained from both domestic (Bombyx mori) and wild (Antheraea pernyi) silkworms from aqueous silk fibroin solutions at room temperature. The gelation time of theAntheraea pernyi(A. pernyi) SF solution was significantly shorter than that of theBombyx mori(B. mori) SF solution. The secondary structures of the two kinds of hydrogels were also compared. In order to further reduce the gelation time, various amounts of polyethylene glycol (PEG) were blended with the silk fibroins ofA. pernyiandB. mori. The gelation time of bothA. pernyiSF andB. moriSF decreased with the increased amount of PEG. After freeze-drying, the hydrogels were characterized through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Results showed that the addition of polyalcohol did not change the main secondary structure of the hydrogels. However, the addition of polyalcohol did reduce the gelation time and triggered additional formation ofβ-sheets.

Ultrasonication-Induced Rapid Gelation of Wild Silkworm Silk Fibroins

2013 ◽  
Vol 634-638 ◽  
pp. 1165-1169 ◽  
Author(s):  
Gui Yang Liu ◽  
Si Yong Xiong ◽  
Ren Chuan You ◽  
Ling Shuang Wang ◽  
Ming Zhong Li
Keyword(s):  
Secondary Structure ◽  
Silk Fibroin ◽  
Gelation Time ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Freeze Dried ◽  
Silk Fibroins ◽  
Wild Silkworm ◽  
Α Helix ◽  
Silkworm Silk

Silk fibroin （SF） hydrogels of the wild silkworm species Antheraea pernyi and Antheraea yamamai were obtained from aqueous SF solutions at room temperature. Both A. pernyi and A. yamamai solutions were slow to gelate. Hydrogels of the two species of wild silkworm were obtained rapidly following ultrasonicaton at 400–500 W. The secondary structure of the freeze-dried SF hydrogels was measured by X-ray diffraction and Fourier transform infrared spectroscopy. Ultrasonication did not change the main secondary structure of the hydrogels, but it accelerated the structural transformation of silk fibroin molecules from random coil or α helix to β sheet and reduced the gelation time.

scholarly journals Phosphorylation of silk fibroins improves the cytocompatibility of silk fibroin derived materials: A platform for the production of tuneable material

2014 ◽  
Vol 9 (10) ◽  
pp. 1267-1278 ◽  
Author(s):  
Vadim Volkov ◽  
Andreia Vasconcelos ◽  
Marisa P. Sárria ◽  
Andreia C. Gomes ◽  
Artur Cavaco-Paulo
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroins
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