Introduction of VEGF or RGD sequences improves revascularization properties of Bombyx mori silk fibroin produced by transgenic silkworm

Transgenic silk fibroins incorporated the VEGF and RGD were prepared. The VEGF SF showed lower platelet adhesion than the RGD SF and WT SF. An in vivo implantation study supported these in vitro results.

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Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth

Polymers ◽

10.3390/polym13234182 ◽

2021 ◽

Vol 13 (23) ◽

pp. 4182

Author(s):

Chavee Laomeephol ◽

Apichai Vasuratna ◽

Juthamas Ratanavaraporn ◽

Sorada Kanokpanont ◽

Jittima Amie Luckanagul ◽

...

Keyword(s):

Bombyx Mori ◽

Silk Fibroin ◽

Biomedical Applications ◽

Spider Silk ◽

Gelation Time ◽

Cellular Interactions ◽

Spider Silk Protein ◽

Biological Tests ◽

Bombyx Mori Silk

Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications.

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In vitro Degradation Behavior of Bombyx mori Silk Fibroin Films Exposure to Protease XXIII

Biotechnology(Faisalabad) ◽

10.3923/biotech.2009.468.472 ◽

2009 ◽

Vol 8 (4) ◽

pp. 468-472 ◽

Cited By ~ 1

Author(s):

K. Nuanchai ◽

S. Prasong ◽

S. Wilaiwan

Keyword(s):

Bombyx Mori ◽

Silk Fibroin ◽

Degradation Behavior ◽

In Vitro Degradation ◽

Bombyx Mori Silk

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Desmopressin (DDAVP) Enhances Platelet Adhesion to the Extracellular Matrix of Cultured Human Endothelial Cells through Increased Expression of Tissue Factor

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656088 ◽

1997 ◽

Vol 77 (05) ◽

pp. 0975-0980 ◽

Cited By ~ 23

Author(s):

Angel Gálvez ◽

Goretti Gómez-Ortiz ◽

Maribel Díaz-Ricart ◽

Ginés Escolar ◽

Rogelio González-Sarmiento ◽

...

Keyword(s):

Extracellular Matrix ◽

Endothelial Cells ◽

Tissue Factor ◽

Platelet Adhesion ◽

Umbilical Vein ◽

Procoagulant Activity ◽

Experimental Conditions ◽

Chromogenic Assay

SummaryThe effect of desmopressin (DDAVP) on thrombogenicity, expression of tissue factor and procoagulant activity (PCA) of extracellular matrix (ECM) generated by human umbilical vein endothelial cells cultures (HUVEC), was studied under different experimental conditions. HUVEC were incubated with DDAVP (1, 5 and 30 ng/ml) and then detached from their ECM. The reactivity towards platelets of this ECM was tested in a perfusion system. Coverslips covered with DD A VP-treated ECMs were inserted in a parallel-plate chamber and exposed to normal blood anticoagulated with low molecular weight heparin (Fragmin®, 20 U/ml). Perfusions were run for 5 min at a shear rate of 800 s1. Deposition of platelets on ECMs was significantly increased with respect to control ECMs when DDAVP was used at 5 and 30 ng/ml (p <0.05 and p <0.01 respectively). The increase in platelet deposition was prevented by incubation of ECMs with an antibody against human tissue factor prior to perfusion. Immunofluorescence studies positively detected tissue factor antigen on DDAVP derived ECMs. A chromogenic assay performed under standardized conditions revealed a statistically significant increase in the procoagulant activity of the ECMs produced by ECs incubated with 30 ng/ml DDAVP (p <0.01 vs. control samples). Northern blot analysis revealed increased levels of tissue factor mRNA in extracts from ECs exposed to DDAVP. Our data indicate that DDAVP in vitro enhances platelet adhesion to the ECMs through increased expression of tissue factor. A similar increase in the expression of tissue factor might contribute to the in vivo hemostatic effect of DDAVP.

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Evaluation of Structure-Controlled Silk Fibroin Coatings for Orthopedic Infection and In-Situ Osteogenesis: In Vitro and In Vivo

SSRN Electronic Journal ◽

10.2139/ssrn.3600190 ◽

2020 ◽

Author(s):

Wenhao Zhou ◽

Teng Zhang ◽

Jianglong Yan ◽

QiYao Li ◽

Panpan Xiong ◽

...

Keyword(s):

Silk Fibroin ◽

Orthopedic Infection

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Oxygen Permeability of Silk Fibroin Hydrogels and Their Use as Materials for Contact Lenses: A Purposeful Analysis

Gels ◽

10.3390/gels7020058 ◽

2021 ◽

Vol 7 (2) ◽

pp. 58

Author(s):

Traian V. Chirila

Keyword(s):

Tissue Engineering ◽

Bombyx Mori ◽

Silk Fibroin ◽

Biomedical Applications ◽

Oxygen Permeability ◽

Contact Lenses ◽

The Past ◽

Fibrous Protein ◽

Silk Moth ◽

Bombyx Mori Silk

Fibroin is a fibrous protein that can be conveniently isolated from the silk cocoons produced by the larvae of Bombyx mori silk moth. In its form as a hydrogel, Bombyx mori silk fibroin (BMSF) has been employed in a variety of biomedical applications. When used as substrates for biomaterial-cells constructs in tissue engineering, the oxygen transport characteristics of the BMSF membranes have proved so far to be adequate. However, over the past three decades the BMSF hydrogels have been proposed episodically as materials for the manufacture of contact lenses, an application that depends on substantially elevated oxygen permeability. This review will show that the literature published on the oxygen permeability of BMSF is both limited and controversial. Additionally, there is no evidence that contact lenses made from BMSF have ever reached commercialization. The existing literature is discussed critically, leading to the conclusion that BMSF hydrogels are unsuitable as materials for contact lenses, while also attempting to explain the scarcity of data regarding the oxygen permeability of BMSF. To the author’s knowledge, this review covers all publications related to the topic.

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Recombinant silk fibroin incorporated cell-adhesive sequences produced by transgenic silkworm as a possible candidate for use in vascular graft

Journal of Materials Chemistry B ◽

10.1039/c4tb01301h ◽

2014 ◽

Vol 2 (42) ◽

pp. 7375-7383 ◽

Cited By ~ 16

Author(s):

Tetsuo Asakura ◽

Makoto Isozaki ◽

Toshiki Saotome ◽

Ken-ichiro Tatematsu ◽

Hideki Sezutsu ◽

...

Keyword(s):

Silk Fibroin ◽

Vascular Graft ◽

Transgenic Silkworm ◽

Cell Adhesive ◽

Silk Fibroins

Transgenic silk fibroins that incorporated the laminin sequence were prepared. The adhesive activities tend to increase in the TG silk fibroins relative to WT silk fibroins.

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