In situ single molecule detection of insulin receptors on erythrocytes from a type 1 diabetes ketoacidosis patient by atomic force microscopy

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7407-7416 ◽  
Author(s):  
Lu Zhang ◽  
Jiang Pi ◽  
Qiping Shi ◽  
Jiye Cai ◽  
Peihui Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Type 1 Diabetes ◽  
Single Molecule ◽  
Insulin Receptors ◽  
Single Molecule Detection ◽  
Force Microscopy ◽  
Atomic Force ◽  
Molecule Interactions

A method to investigate the single molecule interactions between insulin and insulin receptor in erythrocytes from healthy volunteer and type 1 diabetes ketoacidosis (T1-DKA) patient was introduced using atomic force microscopy (AFM).

scholarly journals Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4798
Author(s):  
Yang Xin ◽  
Amir Ardalan Zargariantabrizi ◽  
Guido Grundmeier ◽  
Adrian Keller
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Pure Water ◽  
Dna Origami ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Individual ◽  
Individual Strategies ◽  
Phosphate Buffered Saline

DNA origami nanostructures (DONs) are promising substrates for the single-molecule investigation of biomolecular reactions and dynamics by in situ atomic force microscopy (AFM). For this, they are typically immobilized on mica substrates by adding millimolar concentrations of Mg2+ ions to the sample solution, which enable the adsorption of the negatively charged DONs at the like-charged mica surface. These non-physiological Mg2+ concentrations, however, present a serious limitation in such experiments as they may interfere with the reactions and processes under investigation. Therefore, we here evaluate three approaches to efficiently immobilize DONs at mica surfaces under essentially Mg2+-free conditions. These approaches rely on the pre-adsorption of different multivalent cations, i.e., Ni2+, poly-l-lysine (PLL), and spermidine (Spdn). DON adsorption is studied in phosphate-buffered saline (PBS) and pure water. In general, Ni2+ shows the worst performance with heavily deformed DONs. For 2D DON triangles, adsorption at PLL- and in particular Spdn-modified mica may outperform even Mg2+-mediated adsorption in terms of surface coverage, depending on the employed solution. For 3D six-helix bundles, less pronounced differences between the individual strategies are observed. Our results provide some general guidance for the immobilization of DONs at mica surfaces under Mg2+-free conditions and may aid future in situ AFM studies.

In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami

Nanoscale ◽  
2011 ◽  
Vol 3 (6) ◽  
pp. 2481 ◽  
Author(s):  
Na Wu ◽  
Xingfei Zhou ◽  
Daniel M. Czajkowsky ◽  
Ming Ye ◽  
Dongdong Zeng ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Time Lapse ◽  
Dna Origami ◽  
In Situ Monitoring ◽  
Force Microscopy ◽  
Atomic Force

Single-molecule imaging of DNA polymerase I (Klenow fragment) activity by atomic force microscopy

Nanoscale ◽  
2016 ◽  
Vol 8 (11) ◽  
pp. 5842-5846 ◽  
Author(s):  
J. Chao ◽  
P. Zhang ◽  
Q. Wang ◽  
N. Wu ◽  
F. Zhang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Polymerase ◽  
Single Molecule ◽  
Klenow Fragment ◽  
Dna Polymerase I ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Polymerase I

Observing DNA replicationin situat the single-molecule level by atomic force microscopy.

In Situ Detection of Liver Cancer Biomarkers with Atomic Force Microscopy at a Single-molecule Level

2013 ◽  
Vol 42 (10) ◽  
pp. 1154-1156 ◽  
Author(s):  
Bochong Tang ◽  
Min Li ◽  
Xiao Zhang ◽  
Jiaojiao Wang ◽  
Peng Xie ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Liver Cancer ◽  
Single Molecule ◽  
Cancer Biomarkers ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
In Situ Detection

scholarly journals Taking nanomedicine teaching into practice with atomic force microscopy and force spectroscopy

2015 ◽  
Vol 39 (4) ◽  
pp. 360-366 ◽  
Author(s):  
Filomena A. Carvalho ◽  
Teresa Freitas ◽  
Nuno C. Santos
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Molecular Mechanisms ◽  
Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force ◽  
Human Blood Cells ◽  
Hands On ◽  
Molecule Interactions ◽  
Near Future

Atomic force microscopy (AFM) is a useful and powerful tool to study molecular interactions applied to nanomedicine. The aim of the present study was to implement a hands-on atomic AFM course for graduated biosciences and medical students. The course comprises two distinct practical sessions, where students get in touch with the use of an atomic force microscope by performing AFM scanning images of human blood cells and force spectroscopy measurements of the fibrinogen-platelet interaction. Since the beginning of this course, in 2008, the overall rating by the students was 4.7 (out of 5), meaning a good to excellent evaluation. Students were very enthusiastic and produced high-quality AFM images and force spectroscopy data. The implementation of the hands-on AFM course was a success, giving to the students the opportunity of contact with a technique that has a wide variety of applications on the nanomedicine field. In the near future, nanomedicine will have remarkable implications in medicine regarding the definition, diagnosis, and treatment of different diseases. AFM enables students to observe single molecule interactions, enabling the understanding of molecular mechanisms of different physiological and pathological processes at the nanoscale level. Therefore, the introduction of nanomedicine courses in bioscience and medical school curricula is essential.

Atomic Force Microscopy (AFM) for Topography and Recognition Imaging at Single Molecule Level

2013 ◽  
pp. 102-112
Author(s):  
Memed Duman ◽  
Andreas Ebner ◽  
Christian Rankl ◽  
Jilin Tang ◽  
Lilia A. Chtcheglova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recognition Imaging
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