Monoclonal antibody–europium conjugate-based lateral flow time-resolved fluoroimmunoassay for quantitative determination of T-2 toxin in cereals and feed

A method of lateral flow time-resolved fluoroimmunoassay (LF-TRFIA) was developed for rapid and ultrasensitive detection of T-2 toxin with TRFIA strips and an assorted portable TRFIA reader.

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Quantitative determination of trace metals in single yeast cells by time-resolved ICP-MS using dissolved standards for calibration

Applied Microbiology and Biotechnology ◽

10.1007/s00253-018-09587-w ◽

2019 ◽

Vol 103 (3) ◽

pp. 1475-1483 ◽

Cited By ~ 7

Author(s):

Zihui Liu ◽

Aifang Xue ◽

Hao Chen ◽

Shengqing Li

Keyword(s):

Trace Metals ◽

Quantitative Determination ◽

Yeast Cells ◽

Time Resolved ◽

Icp Ms

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Carbon nanotube-based lateral flow immunoassay for ultrasensitive detection of proteins: application to the determination of IgG

Microchimica Acta ◽

10.1007/s00604-019-3508-4 ◽

2019 ◽

Vol 186 (7) ◽

Cited By ~ 6

Author(s):

Wanwei Qiu ◽

Kwaku Baryeh ◽

Sunitha Takalkar ◽

Wei Chen ◽

Guodong Liu

Keyword(s):

Carbon Nanotube ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

Ultrasensitive Detection

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A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator

Analytical Biochemistry ◽

10.1016/j.ab.2019.113384 ◽

2019 ◽

Vol 584 ◽

pp. 113384 ◽

Cited By ~ 1

Author(s):

Michał Szkop ◽

Kamil Brygoła ◽

Magdalena Janczewska ◽

Tomasz Ciach

Keyword(s):

Quantitative Determination ◽

Fluorescence Assay ◽

Time Resolved Fluorescence ◽

Time Resolved

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Direct Assay for Cobalamin Bound to Transcobalamin (Holo-Transcobalamin) in Serum

Clinical Chemistry ◽

10.1093/clinchem/48.3.526 ◽

2002 ◽

Vol 48 (3) ◽

pp. 526-532 ◽

Cited By ~ 87

Author(s):

Marius Ulleland ◽

Ingar Eilertsen ◽

Edward V Quadros ◽

Sheldon P Rothenberg ◽

Sergey N Fedosov ◽

...

Keyword(s):

Monoclonal Antibody ◽

Detection Limit ◽

Quantitative Determination ◽

Reliable Method ◽

Solid Phase ◽

Reference Interval ◽

Magnetic Microspheres ◽

Affinity Constant ◽

Capture Assay

Abstract Background: Only cobalamin carried by transcobalamin (holo-transcobalamin) is available for cellular uptake and hence is physiologically relevant. However, no reliable or accurate methods for quantifying holo-transcobalamin are available. We report a novel holo-transcobalamin assay based on solid-phase capture of transcobalamin. Methods: A monoclonal antibody specific for human transcobalamin with an affinity constant >1010 L/mol was immobilized on magnetic microspheres to capture and concentrate transcobalamin. The cobalamin bound to transcobalamin was then released and assayed by a competitive binding radioassay. The quantification of holo-transcobalamin was accomplished using calibrators composed of recombinant, human holo-transcobalamin. Results: The assay was specific for holo-transcobalamin and had a detection limit of 5 pmol/L. Within-run and total imprecision (CV) was 5% and 8–9%, respectively. The working range (CV <20%) was 5–370 pmol/L. Dilutions of serum were linear in the assay range. The recovery of recombinant, human holo-transcobalamin added to serum was 93–108%. A 95% reference interval of 24–157 pmol/L was established for holo-transcobalamin in 105 healthy volunteers 20–80 years of age. For 72 of these sera, holo-haptocorrin and total cobalamin were also determined. Whereas holo-haptocorrin correlated well (r2 = 0.87) with total cobalamin, holo-transcobalamin correlated poorly (r2 = 0.23) with total cobalamin or holo-haptocorrin. Conclusions: The solid-phase capture assay provides a simple, reliable method for quantitative determination of holo-transcobalamin in serum.

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