Photo-click construction of a targetable and activatable two-photon probe imaging protease in apoptosis

2016 ◽  
Vol 52 (11) ◽  
pp. 2342-2345 ◽  
Author(s):  
Mi Zhou ◽  
Jing Hu ◽  
Mengmeng Zheng ◽  
Qinhua Song ◽  
Jinbo Li ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Two Photon ◽  
Apoptosis Imaging

Targetable and activatable two-photon probes constructed using photo-click chemistry were conducted in mitochondria, lysosome and apoptosis imaging.

Fluorescent periodic mesoporous organosilica nanoparticles dual-functionalized via click chemistry for two-photon photodynamic therapy in cells

2016 ◽  
Vol 4 (33) ◽  
pp. 5567-5574 ◽  
Author(s):  
Jonas G. Croissant ◽  
Sébastien Picard ◽  
Dina Aggad ◽  
Maxime Klausen ◽  
Chiara Mauriello Jimenez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Click Chemistry ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Periodic Mesoporous Organosilica ◽  
Two Photon ◽  
Mesoporous Organosilica ◽  
Photon Imaging ◽  
In Cells

The synthesis of ethenylene-based periodic mesoporous organosilica nanoparticles for two-photon imaging and photodynamic therapy of breast cancer cells is described.

New chromophores from click chemistry for two-photon absorption and tuneable photoluminescence

2005 ◽  
pp. 2029 ◽  
Author(s):  
Manuel Parent ◽  
Olivier Mongin ◽  
Kenji Kamada ◽  
Claudine Katan ◽  
Mireille Blanchard-Desce
Keyword(s):  
Click Chemistry ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon

scholarly journals A Click Chemistry Strategy for the Synthesis of Efficient Photoinitiators for Two‐Photon Polymerization

2020 ◽  
Vol 30 (50) ◽  
pp. 2006108
Author(s):  
Irene Henning ◽  
Adam W. Woodward ◽  
Graham A. Rance ◽  
Benjamin T. Paul ◽  
Ricky D. Wildman ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Two Photon ◽  
Two Photon Polymerization

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two-photon excitation fluorescence microscopy in living systems

1993 ◽  
Vol 51 ◽  
pp. 154-155 ◽  
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Microscopy ◽  
Singlet State ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation fluorescence microscopy provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In our fluorescence experiments, the final excited state is the same singlet state that is populated during a conventional fluorescence experiment. Thus, the fluorophore exhibits the same emission properties (e.g. wavelength shifts, environmental sensitivity) used in typical biological microscopy studies. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Second order interference in two photon absorption

1996 ◽  
Vol 43 (9) ◽  
pp. 1765-1771 ◽  
Author(s):  
M. W. HAMILTON and D. S. ELLIOTT
Keyword(s):  
Second Order ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon
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