Investigating the adduct formation of organic mercury species with carbonic anhydrase and hemoglobin from human red blood cell hemolysate by means of LC/ESI-TOF-MS and LC/ICP-MS

Metallomics ◽  
2016 ◽  
Vol 8 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Jens Hogeback ◽  
Miriam Schwarzer ◽  
Christoph A. Wehe ◽  
Michael Sperling ◽  
Uwe Karst
Keyword(s):  
Carbonic Anhydrase ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Adduct Formation ◽  
Mercury Species ◽  
Organic Mercury ◽  
Icp Ms ◽  
Tof Ms

scholarly journals Changes in the red blood cell enzyme, carbonic anhydrase, following blood transfusion.

1981 ◽  
Vol 27 (6) ◽  
pp. 541-545
Author(s):  
Kenji Taki ◽  
Koji Murakami ◽  
Takae Kawamura ◽  
Mieko Takamura ◽  
Reiji Wakusawa
Keyword(s):  
Blood Transfusion ◽  
Carbonic Anhydrase ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Enzyme

scholarly journals Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum

2020 ◽  
Vol 223 (22) ◽  
pp. jeb232991
Author(s):  
Angelina M. Dichiera ◽  
Andrew J. Esbaugh
Keyword(s):  
Carbonic Anhydrase ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Fold Increase ◽  
Red Drum ◽  
Sciaenops Ocellatus ◽  
Scaling Exponent ◽  
Root Effect ◽  
Tightly Coupled

ABSTRACTOxygen (O2) and carbon dioxide (CO2) transport are tightly coupled in many fishes as a result of the presence of Root effect hemoglobins (Hb), whereby reduced pH reduces O2 binding even at high O2 tensions. Red blood cell carbonic anhydrase (RBC CA) activity limits the rate of intracellular acidification, yet its role in O2 delivery has been downplayed. We developed an in vitro assay to manipulate RBC CA activity while measuring Hb-O2 offloading following a physiologically relevant CO2-induced acidification. RBC CA activity in red drum (Sciaenops ocellatus) was inhibited with ethoxzolamide by 53.7±0.5%, which prompted a significant reduction in O2 offloading rate by 54.3±5.4% (P=0.0206, two-tailed paired t-test; n=7). Conversely, a 2.03-fold increase in RBC CA activity prompted a 2.14-fold increase in O2 offloading rate (P<0.001, two-tailed paired t-test; n=8). This approximately 1:1 relationship between RBC CA activity and Hb-O2 offloading rate coincided with a similar allometric scaling exponent for RBC CA activity and maximum metabolic rate. Together, our data suggest that RBC CA is rate limiting for O2 delivery in red drum.

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