A label-free fluorescence method for the detection of uracil DNA glycosylase activity based on G-quadruplex formation

2016 ◽  
Vol 8 (41) ◽  
pp. 7453-7459 ◽  
Author(s):  
Changbei Ma ◽  
Kefeng Wu ◽  
Jun Wang ◽  
Hailun He ◽  
Feng Ning ◽  
...  
Keyword(s):  
Fluorescence Method ◽  
Thioflavin T ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Quadruplex Formation

A simple fluorescence based biosensor for label-free detection of uracil DNA glycosylase activity based on G-quadruplex formation using a thioflavin T probe is reported.

Label-free fluorescence turn-on detection of uracil DNA glycosylase activity based on G-quadruplex formation

Talanta ◽  
2016 ◽  
Vol 160 ◽  
pp. 449-453 ◽  
Author(s):  
Changbei Ma ◽  
Kefeng Wu ◽  
Haisheng Liu ◽  
Kun Xia ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Turn On ◽  
G Quadruplex ◽  
Quadruplex Formation ◽  
Fluorescence Turn On

scholarly journals Comparison of DNAzyme activity for the development of an immobilized heme sensor

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1491-1496
Author(s):  
Natalie Hughes ◽  
Nancy Nguyen ◽  
Deanna-Kaye Daley ◽  
Justin Grennell ◽  
Amira Gee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Label Free ◽  
Detection Range ◽  
Chemical Complexity ◽  
Detection Strategy ◽  
Detection Approach ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Care Systems

ABSTRACTPoint-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

scholarly journals Label Free Detection of Adenosine Based on Hemin/G-Quadruplex Peroxidase-Mimicking DNAzyme as Bioelectrocatalyst

2013 ◽  
Vol 25 (18) ◽  
pp. 10541-10545 ◽  
Author(s):  
Yu Li ◽  
Sen Li ◽  
Ge-Qing Xia ◽  
Yuansheng Wang
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex

Cationic-perylene-G-quadruplex complex based fluorescent biosensor for label-free detection of Pb 2+

Talanta ◽  
2016 ◽  
Vol 149 ◽  
pp. 98-102 ◽  
Author(s):  
Xu-Hua Zhao ◽  
Liang Gong ◽  
Yuan Wu ◽  
Xiao-Bing Zhang ◽  
Jun Xie
Keyword(s):  
Label Free ◽  
Fluorescent Biosensor ◽  
Label Free Detection ◽  
G Quadruplex

Parallel [TG(GA)3]n-homoduplexes/thioflavin T: an intense and stable fluorescent indicator for label-free biosensing

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 286-294 ◽  
Author(s):  
Qiang Liu ◽  
Shaochun Jing ◽  
Mei Liu ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Thioflavin T ◽  
Label Free ◽  
Fluorescent Indicator ◽  
G Quadruplex

Compared with the G-quadruplex/ThT fluorescent system, parallel [TG(GA)3]n-dsDNA/ThT is a stable and strong fluorescent indicator for label-free biosensing.

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