The effect of gold nanoparticles on the diagnostic polymerase chain reaction technique for equine herpes virus 1 (EHV-1)

RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54898-54903 ◽  
Author(s):  
Dalia M. El-Husseini ◽  
Nashwa M. Helmy ◽  
Reham H. Tammam
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Herpes Virus ◽  
Polymerase Chain Reaction Technique ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

We experimented the effect of 15 nm unmodified citrate coated GNPs on the key PCR reactants to see if these would enhance the overall outcomes of the reaction. Thus, the optimized GNPs-assisted PCR could be used for more efficient diagnosis of EHV-1.

scholarly journals DETEKSI KOI HERPES VIRUS DENGAN METODE POLYMERASE CHAIN REACTION

2016 ◽  
Vol 14 (2) ◽  
pp. 115
Author(s):  
Dini Sahfitri Lubis ◽  
Diah Artati
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Virus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

Polymerase chain reaction (PCR) merupakan salah satu metode deteksi cepat koi herpes vrus (KHV). Metode PCR ini sangat sensitif. Sensitivitas tersebut membuatnya dapat digunakan untuk melipatgandakan satu molekul DNA. Konsentrasi dan kualitas DNA dipengaruhi oleh keberhasilan pada saat melakukan ekstraksi DNA. Kegiatan percobaan ini bertujuan untuk mengetahui hasil deteksi virus KHV dengan metode PCR. Keberhasilan analisis PCR sangat dipengaruhi karakteristik DNA genom yang meliputi kemurnian, konsentrasi dan ukuran template. Virus KHV dideteksi menggunakan metode PCR sesuai dengan SNI 7547:2009. Hasil kegiatan deteksi virus KHV menggunakan PCR dari sampel kode I.230–I.286 dapat memvisualisasikan hasil PCR dengan positif KHV dengan ukuran fragmen DNA 290 bp dengan baik. Dapat dilihat dari 57 sampel yang telah diuji memiliki hasil yang berbeda-beda. Hasil uji yang diperoleh yaitu 32 sampel ikan positif terinfeksi KHV dan 25 sampel negatif KHV

scholarly journals Diagnosis of Equine Herpes Virus 4 Infection using Polymerase Chain Reaction

2020 ◽  
Vol 9 (11) ◽  
pp. 887-890
Author(s):  
J. A. Vala ◽  
M. D. Patel ◽  
D. R. Patel ◽  
U. V. Ramani ◽  
I. H. Kalyani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Virus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

Transient Posttransplant Graft-versus-Host Lymphadenopathy

2004 ◽  
Vol 7 (5) ◽  
pp. 533-537 ◽  
Author(s):  
Laurentia Nodit ◽  
Noriko Murase ◽  
Jorge D. Reyes ◽  
George V. Mazariegos ◽  
Rakesh Sindhi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Virus ◽  
Human Herpes ◽  
Chain Reaction ◽  
Cell Hyperplasia ◽  
Human Herpes Virus 8 ◽  
Human Herpes Virus ◽  
Donor Cells ◽  
Polymerase Chain ◽  
Herpès Virus

There is sparse information in humans on graft-versushost (GVH) lymphadenopathy. A 15-month-old male received a liver and small bowel transplant for short bowel after gastroschisis. At 21 days he developed a GVH-like skin rash. Flow cytometry demonstrated 16.1% circulating donor cells. Polymerase chain reaction for Epstein-Barr virus was negative. Two months later, the rash recurred with diffuse lymphadenopathy. Lymph node biopsy showed effaced architecture without visible follicles, large numbers of CD79a+ immunoblasts interspersed with smaller CD3+ and CD8+ cells, and prominent dendritic cell hyperplasia. Human herpes virus 8, cytomegalovirus, and EBER-1 probes were negative, as was polymerase chain reaction for human herpes virus 6. Allograft intestinal biopsies on days 10 and 24 had a similar infiltrate. The features appeared to be those of lymphocytes trafficking between the graft and host with a mixed lymphocyte reaction in situ, a GVH-type reaction without tissue damage. The reaction was self-limiting in the intestinal graft, and the lymphadenopathy resolved with some decrease in immunosupression. Circulating donor cells fell to 2.5% by day 62, and the child has been rejection free on low-dose immunosuppression.

scholarly journals DETERMINASI MOLEKULER KOI HERPES VIRUS (KHV) YANG DIISOLASI DARI IKAN KOI (Cyprinus carpio koi)

2012 ◽  
Vol 8 (2) ◽  
pp. 64
Author(s):  
Jetti Treslah Saselah ◽  
Reiny A Tumbol ◽  
Henky Manoppo
Keyword(s):  
Polymerase Chain Reaction ◽  
Cyprinus Carpio ◽  
Herpes Virus ◽  
Clinical Symptoms ◽  
The Body ◽  
Chain Reaction ◽  
Fish Samples ◽  
Polymerase Chain ◽  
Herpès Virus ◽  
Cyprinus Carpio Koi

Penelitian ini bertujuan untuk mendeterminasi keberadaan Koi Herpes Virus (KHV) pada ikan koi (Cyprinus carpio koi). Sampel ikan diambil dari Kabupaten Kepulauan Sangihe. Penelitian di lakukan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi dengan menggunakan metode Polymerase Chain Reaction (PCR). Hasil pemeriksaan PCR terhadap sampel ikan koi mengindikasikan bahwa sampel ikan koi telah terinfeksi oleh virus KHV, yang ditandai dengan munculnya pita DNA pada hasil visualisasi elektroforesis agarosa. Hasil pemeriksaan pada morfologi ternyata sampel ikan yang digunakan menunjukkan gejala-gejala klinis terserang KHV seperti mata pucat, insang berwarna pucat serta produksi lendir yang berlebihan. Beberapa sampel lainnya walaupun secara morfologi belum menunjukan gejala-gejala klinis tetapi melalui pemeriksaan PCR telah terindikasikan terinfeksi KHV. Kata kunci: KHV, PCR, ikan koi   The purpose of this study was to determine the presence of Koi Herpes Virus (KHV) in Koi (Cyprinus carpio koi). Fish samples were taken from the Sangihe Archipelago Regency. Laboratory work conducted at the Laboratory of Biology Faculty of Mathematics and Natural Sciences University of Sam Ratulangi using Polymerase Chain Reaction (PCR) method. The samples indicated that the koi fish samples were infected with KHV. It is shown by the appearance of DNA bands on agarose electrophore­sis visualization. The morphological examination of the body indicated clinical symptoms of KHV infection, such as pale eyes, pale gills and excessive mucus production. Several other samples, although without clinical symptoms, have shown to be infected as indicated by PCR test. Keywords: KHV, PCR, Koi.

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