Partitioning behavior of recombinant lipase in Escherichia coli by ionic liquid-based aqueous two-phase systems

RSC Advances ◽  
2016 ◽  
Vol 6 (86) ◽  
pp. 82571-82580 ◽  
Author(s):  
Muhammad Hakimi Hadzir ◽  
Sahar Abbasiliasi ◽  
Arbakariya B. Ariff ◽  
Siti Baidurah Yusoff ◽  
Hui Suan Ng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Fermentation Broth ◽  
Two Phase ◽  
E Coli ◽  
Recombinant Lipase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

Evaluations of ILATPSs were performed with a variety of ionic liquids and salts as phase components to figure out their competencies in the recovery of lipase from a fermentation broth of E. coli using banana waste as a substrate.

scholarly journals Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Zhiwen Bai ◽  
Yanhong Chao ◽  
Meiling Zhang ◽  
Changri Han ◽  
Wenshuai Zhu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Efficiency ◽  
Phase System ◽  
Optimum Conditions ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.

scholarly journals Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli

2018 ◽  
Vol 6 ◽  
Author(s):  
Matthew F. Yee ◽  
Grace N. Emmel ◽  
Eric J. Yang ◽  
Eumene Lee ◽  
Justin H. Paek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionic Liquid ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Synthesis, magnetism, aqueous-two phase formation and physical properties of novel guanidinium-based magnetic ionic liquids

RSC Advances ◽  
2016 ◽  
Vol 6 (58) ◽  
pp. 52898-52904 ◽  
Author(s):  
Tian Yao ◽  
Shun Yao ◽  
Dan Tang ◽  
Long Jing ◽  
Daocai Wang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Physical Properties ◽  
Phase Formation ◽  
Two Phase ◽  
Magnetic Ionic Liquids ◽  
Aqueous Two Phase Systems ◽  
Magnetic Ionic Liquid ◽  
Phase Systems

A novel magnetic ionic liquid aqueous two-phase systems (MILATPs) has been developed, based on a series of novelly synthesized guanidinium-based magnetic ionic liquids.

Partitioning Behavior of Wastewater Proteins in Some Ionic Liquids-Based Aqueous Two-Phase Systems

2012 ◽  
Vol 47 (2) ◽  
pp. 277-283 ◽  
Author(s):  
Yuanchao Pei ◽  
Lin Li ◽  
Zhiyong Li ◽  
Changzeng Wu ◽  
Jianji Wang
Keyword(s):  
Ionic Liquids ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

High-performance extraction of alkaloids using aqueous two-phase systems with ionic liquids

2010 ◽  
Vol 12 (10) ◽  
pp. 1715 ◽  
Author(s):  
Mara G. Freire ◽  
Catarina M. S. S. Neves ◽  
Isabel M. Marrucho ◽  
José N. Canongia Lopes ◽  
Luís Paulo N. Rebelo ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
High Performance ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Thermodynamic studies of partitioning behavior of lysozyme and conalbumin in aqueous two-phase systems

2009 ◽  
Vol 877 (24) ◽  
pp. 2579-2584 ◽  
Author(s):  
Rita de Cássia Superbi de Sousa ◽  
Jane Sélia dos Reis Coimbra ◽  
Luis Henrique Mendes da Silva ◽  
Maria do Carmo Hespanhol da Silva ◽  
Edwin Elard Garcia Rojas ◽  
...  
Keyword(s):  
Two Phase ◽  
Thermodynamic Studies ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior
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