Evaluations of ILATPSs were performed with a variety of ionic liquids and salts as phase components to figure out their competencies in the recovery of lipase from a fermentation broth of E. coli using banana waste as a substrate.
A novel magnetic ionic liquid aqueous two-phase systems (MILATPs) has been developed, based on a series of novelly synthesized guanidinium-based magnetic ionic liquids.
Despite the fact that ionic liquid-based aqueous two phase systems (ATPSs) have been widely studied for extraction purposes, the adequacy of biodegradable organic salts as salting out agents has been left unexploited.
This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.
Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents