scholarly journals Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Zhiwen Bai ◽  
Yanhong Chao ◽  
Meiling Zhang ◽  
Changri Han ◽  
Wenshuai Zhu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Efficiency ◽  
Phase System ◽  
Optimum Conditions ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.

Partitioning behavior of recombinant lipase in Escherichia coli by ionic liquid-based aqueous two-phase systems

RSC Advances ◽  
2016 ◽  
Vol 6 (86) ◽  
pp. 82571-82580 ◽  
Author(s):  
Muhammad Hakimi Hadzir ◽  
Sahar Abbasiliasi ◽  
Arbakariya B. Ariff ◽  
Siti Baidurah Yusoff ◽  
Hui Suan Ng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Fermentation Broth ◽  
Two Phase ◽  
E Coli ◽  
Recombinant Lipase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

Evaluations of ILATPSs were performed with a variety of ionic liquids and salts as phase components to figure out their competencies in the recovery of lipase from a fermentation broth of E. coli using banana waste as a substrate.

scholarly journals The extraction of Zn(II) in aqueous PEG (1550)-(NH4)2SO4 two-phase system using Cl- ions as extracting agent

2007 ◽  
Vol 72 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Aqueous Solutions ◽  
Extraction Efficiency ◽  
Phase Variation ◽  
Chloride Ions ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Experimental Parameters ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

The extraction of Zn(II) in an aqueous PEG (1550) - (NH4)2SO4 two-phase system as a function of several experimental parameters was studied. PEG-based aqueous two-phase systems are composed of two immiscible phases: a polymer-rich phase and a salt-rich phase, which can be used for extraction experiments. In the absence of a suitable extracting agent, for the system consisting of a mixture of equal volumes of 40 mass% PEG and 40 mass% (NH4)2SO4 aqueous solutions, Zn(II) remained predominantly in the salt-rich phase. Variation of the pH of the salt stock solution did not change very much the extraction efficiency. By adding chloride ions, an enhancement of the Zn(II) extraction was observed. The Zn(II) extraction efficiency in presence of Cl- depends on the acidity of the salt stock solution and on the concentration of chloride ions added into the system.

scholarly journals Partitioning of Cephalexin in Ionic Liquid Aqueous Two-Phase System Composed of 1-Butyl-3-Methylimidazolium Tetrafluoroborate andZnSO4

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Yan Fang Li ◽  
Juan Han ◽  
Yun Wang ◽  
Jing Jing Ma ◽  
Yong Sheng Yan
Keyword(s):  
Ionic Liquid ◽  
Extraction Efficiency ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase System ◽  
Extraction And Separation ◽  
Partitioning Behavior

Ionic liquid aqueous two-phase system (ILATPS) was applied in the extraction and separation of hydrosoluble antibiotics. The partitioning behavior of cephalexin (CEX) in 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4)-ZnSO4aqueous two-phase system was studied by the partitioning parameter of the extraction efficiency. The effect of the volume of [Bmim]BF4, the concentration of ZnSO4, temperature, pH, and the volume of ZnSO4solution was discussed concretely. When the volume of [Bmim]BF4was 2 mL and the concentration of ZnSO4was 35%, the extraction efficiency of CEX could reach 92.64% with pH unadjusted. The effect of the volume of [Bmim]BF4on the extraction efficiency was higher than that of the concentration of ZnSO4. The temperature influenced not only the formation of aqueous two-phase system but also the extraction efficiency of CEX. The target was found to be preferentially extracted to the [Bmim]BF4-rich phase at the pH below 4.3. The partition of CEX to the top phase was enhanced by increasing the volume of [Bmim]BF4, the concentration of ZnSO4, and temperature; however, the partition of CEX to the top phase increased by decreasing the pH.

Synthesis, magnetism, aqueous-two phase formation and physical properties of novel guanidinium-based magnetic ionic liquids

RSC Advances ◽  
2016 ◽  
Vol 6 (58) ◽  
pp. 52898-52904 ◽  
Author(s):  
Tian Yao ◽  
Shun Yao ◽  
Dan Tang ◽  
Long Jing ◽  
Daocai Wang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Physical Properties ◽  
Phase Formation ◽  
Two Phase ◽  
Magnetic Ionic Liquids ◽  
Aqueous Two Phase Systems ◽  
Magnetic Ionic Liquid ◽  
Phase Systems

A novel magnetic ionic liquid aqueous two-phase systems (MILATPs) has been developed, based on a series of novelly synthesized guanidinium-based magnetic ionic liquids.

Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System

2011 ◽  
Vol 138-139 ◽  
pp. 995-1001 ◽  
Author(s):  
Wei Jie Wang ◽  
Xiao Le Zhang ◽  
Chang Bo Xu ◽  
Hong Yan Cheng
Keyword(s):  
High Purity ◽  
Separation Process ◽  
Phase System ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Optimized Conditions ◽  
Potassium Sodium Tartrate ◽  
Partitioning Behavior

In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

scholarly journals Extraction of Fe (III) and Zn (II) by ionic liquids in a system based on polypropylene glycol 425 and sodium chloride.

2020 ◽  
Vol 11 (3-2020) ◽  
pp. 72-75
Author(s):  
I. V. Zinov’eva ◽  
◽  
M. I. Fedorova ◽  
Yu. A. Zakhodyaeva ◽  
A.A. Voshkin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Ionic Liquids ◽  
Sodium Chloride ◽  
Phase System ◽  
Polypropylene Glycol ◽  
Two Phase ◽  
Study Results ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Extraction Of Metals

This work presents the experimental study results of Fe(III) and Zn(II) extraction by ionic liquids based on trioctylmethylammonium chloride and rhodanide in an aqueous two-phase system based on polypropylene glycol 425 and sodium chloride. Based on the results obtained, it was concluded that these extractants are effective for the extraction of metals from aqueous solutions, and aqueous two-phase systems represent a “green” alternative to traditional extraction systems.

Partitioning Behavior of Penicillin G in Aqueous Two Phase System Based on Ionic Liquids

2013 ◽  
Vol 864-867 ◽  
pp. 324-327
Author(s):  
Bin Jiang ◽  
Zhi Biao Feng ◽  
Chun Hong Liu ◽  
Ying Cao Xu ◽  
Dong Mei Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Yield ◽  
Penicillin G ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Partitioning Behavior

An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-Butyl-3-methylimid-azolium tetrafluoroborate ([BmiBF4) and salts for the extraction of penicillin G in this paper. The phase forming abilities of different salts were investigated to choose a suitable salt.The partitioning behavior of penicillin G in the ATPS was investigated.Concentrations of (NH4)2SO4, penicillin G, and [BmiBF4 were evaluated to determine their effects on the extraction yield of penicillin G. It was found that extraction yield strongly depended on the concentration of [BmiBF4, penicillin and (NH4)2SO4. A high extraction yield around 93% was achieved with the following parameters: (NH4)2SO4 20% (wt%), penicillin 40000 ~ 45000 U/mL, [BmiBF4 20% (wt%).

Extraction and stability of selected proteins in ionic liquid based aqueous two phase systems

2014 ◽  
Vol 16 (5) ◽  
pp. 2670-2679 ◽  
Author(s):  
Rupali K. Desai ◽  
Mathieu Streefland ◽  
Rene H. Wijffels ◽  
Michel H. M. Eppink
Keyword(s):  
Ionic Liquid ◽  
Aqueous Solutions ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
The Stability

This article discusses the extraction of Rubisco in an ionic liquid (IL) based aqueous two phase system and the stability of proteins in aqueous solutions of IL.

Sign in / Sign up

Export Citation Format

Share Document