scholarly journals Robust light harvesting by a noisy antenna

2018 ◽  
Vol 20 (6) ◽  
pp. 4360-4372 ◽  
Author(s):  
Pavel Malý ◽  
Alastair T. Gardiner ◽  
Richard J. Cogdell ◽  
Rienk van Grondelle ◽  
Tomáš Mančal
Keyword(s):  
Theoretical Model ◽  
Single Molecule ◽  
Light Harvesting ◽  
Single Molecule Spectroscopy ◽  
Protein Fluctuations

Ultrafast bulk and single-molecule spectroscopy experiments described by a single theoretical model show how protein fluctuations influence photosynthetic light harvesting.

Single Molecule Spectroscopy of Oriented Recombinant Trimeric Light Harvesting Complexes of Higher Plants

2002 ◽  
Vol 3 (4) ◽  
pp. 183-188 ◽  
Author(s):  
Uwe Gerken ◽  
Henriette Wolf-Klein ◽  
Christine Huschenbett ◽  
Britta Götze ◽  
Sebastian Schuler ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Harvesting ◽  
Higher Plants ◽  
Single Molecule Spectroscopy ◽  
Light Harvesting Complexes

The architecture and function of the light-harvesting apparatus of purple bacteria: from single molecules to in vivo membranes

2006 ◽  
Vol 39 (3) ◽  
pp. 227-324 ◽  
Author(s):  
Richard J. Cogdell ◽  
Andrew Gall ◽  
Jürgen Köhler
Keyword(s):  
Quantum Mechanics ◽  
Energy Transfer ◽  
Single Molecule ◽  
Molecular Mechanisms ◽  
Light Harvesting ◽  
Structural Information ◽  
Purple Bacteria ◽  
Theoretical Background ◽  
Single Molecule Spectroscopy

1. Introduction 2292. Structures 2342.1 The structure of LH2 2342.2 Natural variants of peripheral antenna complexes 2422.3 RC–LH1 complexes 2423. Spectroscopy 2493.1 Steady-state spectroscopy 2493.2 Factors which affect the position of the Qy absorption band of Bchla 2494. Regulation of biosynthesis and assembly 2574.1 Regulation 2574.1.1 Oxygen 2574.1.2 Light 2584.1.2.1 AppA: blue-light-mediated regulation 2594.1.2.2 Bacteriophytochromes 2594.1.3 From the RC to the mature PSU 2614.2 Assembly 2614.2.1 LH1 2624.2.2 LH2 2635. Frenkel excitons 2655.1 General 2655.2 B800 2675.3 B850 2675.4 B850 delocalization 2736. Energy-transfer pathways: experimental results 2746.1 Theoretical background 2746.2 ‘Follow the excitation energy’ 2766.2.1 Bchla→Bchla energy transfer 2776.2.1.1 B800→B800 2776.2.1.2 B800→B850 2786.2.1.3 B850→B850 2796.2.1.4 B850→B875 2806.2.1.5 B875→RC 2806.2.2 Car[harr ]Bchla energy transfer 2817. Single-molecule spectroscopy 2847.1 Introduction to single-molecule spectroscopy 2847.2 Single-molecule spectroscopy on LH2 2857.2.1 Overview 2857.2.2 B800 2867.2.2.1 General 2867.2.2.2 Intra- and intercomplex disorder of site energies 2877.2.2.3 Electron-phonon coupling 2897.2.2.4 B800→B800 energy transfer revisited 2907.2.3 B850 2938. Quantum mechanics and the purple bacteria LH system 2989. Appendix 2999.1 A crash course on quantum mechanics 2999.2 Interacting dimers 30510. Acknowledgements 30611. References 307This review describes the structures of the two major integral membrane pigment complexes, the RC–LH1 ‘core’ and LH2 complexes, which together make up the light-harvesting system present in typical purple photosynthetic bacteria. The antenna complexes serve to absorb incident solar radiation and to transfer it to the reaction centres, where it is used to ‘power’ the photosynthetic redox reaction and ultimately leads to the synthesis of ATP. Our current understanding of the biosynthesis and assembly of the LH and RC complexes is described, with special emphasis on the roles of the newly described bacteriophytochromes. Using both the structural information and that obtained from a wide variety of biophysical techniques, the details of each of the different energy-transfer reactions that occur, between the absorption of a photon and the charge separation in the RC, are described. Special emphasis is given to show how the use of single-molecule spectroscopy has provided a more detailed understanding of the molecular mechanisms involved in the energy-transfer processes. We have tried, with the help of an Appendix, to make the details of the quantum mechanics that are required to appreciate these molecular mechanisms, accessible to mathematically illiterate biologists. The elegance of the purple bacterial light-harvesting system lies in the way in which it has cleverly exploited quantum mechanics.

scholarly journals Conformational Switching in a Light-Harvesting Protein as Followed by Single-Molecule Spectroscopy

2015 ◽  
Vol 108 (11) ◽  
pp. 2713-2720 ◽  
Author(s):  
Andrew Gall ◽  
Cristian Ilioaia ◽  
Tjaart P.J. Krüger ◽  
Vladimir I. Novoderezhkin ◽  
Bruno Robert ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Harvesting ◽  
Single Molecule Spectroscopy ◽  
Conformational Switching

Probing the Electronic Structure and Conformational Flexibility of Individual Light-Harvesting 3 Complexes by Optical Single-Molecule Spectroscopy

2006 ◽  
Vol 110 (37) ◽  
pp. 18710-18717 ◽  
Author(s):  
Martijn Ketelaars ◽  
Jean-Manuel Segura ◽  
Silke Oellerich ◽  
Ward P. F. de Ruijter ◽  
Gerhard Magis ◽  
...  
Keyword(s):  
Electronic Structure ◽  
Single Molecule ◽  
Light Harvesting ◽  
Conformational Flexibility ◽  
Single Molecule Spectroscopy

Individual LH3 (B800-820) Light-Harvesting Complexes Studied by Optical Single-Molecule Spectroscopy

2002 ◽  
Vol 3 (5-6) ◽  
pp. 319-320 ◽  
Author(s):  
S. Oellerich ◽  
M. Ketelaars ◽  
J.-M. Segura ◽  
G. Margis ◽  
W. de Ruijter ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Harvesting ◽  
Single Molecule Spectroscopy ◽  
Light Harvesting Complexes

scholarly journals Single Molecule Spectroscopy on the Light-Harvesting Complex II of Higher Plants

2001 ◽  
Vol 81 (1) ◽  
pp. 556-562 ◽  
Author(s):  
Carsten Tietz ◽  
Fedor Jelezko ◽  
Uwe Gerken ◽  
Sebastian Schuler ◽  
Axel Schubert ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Harvesting ◽  
Higher Plants ◽  
Single Molecule Spectroscopy ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

scholarly journals Observation of robust energy transfer in the photosynthetic protein allophycocyanin using single-molecule pump-probe spectroscopy

2021 ◽  
Author(s):  
Raymundo Moya ◽  
Audrey Norris ◽  
Toru Kondo ◽  
Gabriela Schlau-Cohen
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Light Harvesting ◽  
Asymmetric Distribution ◽  
Ultrafast Dynamics ◽  
Pump Probe ◽  
Photosynthetic Organisms ◽  
Probe Spectroscopy ◽  
The Impact ◽  
Protein Fluctuations

Abstract Photosynthetic organisms convert sunlight to electricity with near unity quantum efficiency. Absorbed photoenergy transfers through a network of chromophores positioned within protein scaffolds, which fluctuate due to thermal motion. The resultant variation in energy transfer has not yet been measured, and so how the efficiency is robust to this variation, if any, has not been determined. Here, we describe single-molecule pump-probe spectroscopy with facile spectral tuning and its application to the ultrafast dynamics of single allophycocyanin, a light-harvesting protein from cyanobacteria. Using the spectral dependence of the dynamics, energy transfer and energetic relaxation from nuclear motion were disentangled. For energy transfer, an asymmetric distribution of timescales was observed. For energetic relaxation, the timescales were slower and more heterogeneous due to the impact of the protein environment. Collectively, these results suggest that energy transfer is robust to protein fluctuations, a prerequisite for efficient light harvesting.

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