Real-time tracing the changes in the intracellular pH value during apoptosis by near-infrared ratiometric fluorescence imaging

2018 ◽  
Vol 54 (65) ◽  
pp. 9071-9074 ◽  
Author(s):  
Liyun Lin ◽  
Jingjin Zhao ◽  
Liangliang Zhang ◽  
Yong Huang ◽  
Fanggui Ye ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Near Infrared ◽  
Ph Value ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Induced Apoptosis

A single-excitation dual-emission nanoprobe with near-infrared response was developed for real-time tracing the changes in the pH value during hydrogen peroxide-induced apoptosis by ratiometric fluorescence imaging.

scholarly journals Near-Infrared Dual-Emission Ratiometric Fluorescence Imaging Nanoprobe for Real-Time Tracing the Generation of Endogenous Peroxynitrite in Single Living Cells and In Vivo

ACS Omega ◽  
2020 ◽  
Vol 5 (22) ◽  
pp. 13278-13286
Author(s):  
Pengxiang Lin ◽  
Dongxia Chen ◽  
Liangliang Zhang ◽  
Jiayao Xu ◽  
Yong Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Living Cells ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Single Living Cells ◽  
Single Living

Single-excitation, dual-emission biomass quantum dots: preparation and application for ratiometric fluorescence imaging of coenzyme A in living cells

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9270-9275 ◽  
Author(s):  
Yong Cui ◽  
Rongjun Liu ◽  
Fanggui Ye ◽  
Shulin Zhao
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Fluorescence Detection ◽  
Coenzyme A ◽  
Living Cells ◽  
Fluorescence Probes ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Dual Emissions

A distinctive approach is proposed to prepare biomass quantum dots (BQDs) with single-excitation and dual-emissions property. The obtained BQDs have been employed as the fluorescence probes for ratiometric fluorescence detection and imaging of coenzyme A in living cells.

scholarly journals Intraoperative Near-Infrared Fluorescence Imaging With Second-Window Indocyanine-Green Offers Accurate and Real-Time Correction for Brain Shift and Anatomic Inconsistencies in Framless Neuronavigation

Neurosurgery ◽  
2019 ◽  
Vol 66 (Supplement_1) ◽  
Author(s):  
Steve Sungwon Cho ◽  
Ashwin G Ramayya ◽  
Clare W Teng ◽  
Steven Brem ◽  
Sunil Singhal ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Infrared Imaging ◽  
P Value ◽  
Brain Shift ◽  
Intracranial Tumors ◽  
Near Infrared Imaging ◽  
Near Infrared Fluorescence ◽  
Near Infrared Fluorescence Imaging

Abstract INTRODUCTION Neuronavigation allows neurosurgeons to localize intracranial structures in 3D space and has been shown to have positive impacts on patient survival in neuro-oncology surgery. However, its major limitation is the intraoperative brain-shift phenomenon, in which the brain moves during surgery due to physical, surgical, and/or biological factors, invalidating the preoperative registration and leading to inaccuracies in the cranium. One reliable way to account for brain-shift intraoperatively may be to use tumor-targeting near-infrared fluorophores, such as ICG accumulating in neoplastic tissue (ie, Second-Window ICG), which offers real-time visualization of tumors through normal cortex and dura. METHODS Patients undergoing craniotomy for primary resection of intracranial tumors were enrolled under an ongoing clinical trial investigating the efficacy of SWIG. For this analysis, retrospective data were collected on patients in whom neuronavigation was used to plan the craniotomy in such a way as to place the tumor in the center of the craniotomy (ie, parasagittal tumors, skull-base tumors, and large/asymmetrical tumors were excluded). During surgery, near-infrared imaging was performed before and after durotomy to localize the gross tumor. Image analysis was performed to measure the deviation between the craniotomy center and the center of the tumor as seen with near-infrared fluorescence. RESULTS A total of 63 patients (24 high-grade-gliomas, 12 meningiomas, 25 metastases, 2 others) were included in this preliminary analysis. Neuronavigation demonstrated a median deviation of 22.4% (range 7.7%-44.4%) relative to craniotomy size. Patient position was a significant predictor of neuronavigation inaccuracy, with the prone position having significantly higher inaccuracy (28.5 ± 8.8%) compared to the supine (19.3 ± 9.2%, P-value = .015) or the lateral (17.9 ± 6.6%, P-value = .012) positions. Additionally, the neuronavigation device used and postgraduate training level of the residents performing the registration trended towards significance on multivariate analysis. In contrast, near-infrared fluorescence perfectly delineated the tumor in all cases. CONCLUSION We demonstrate that near-infrared fluorescence imaging offers more accurate localization of intracranial tumors compared to frameless neuronavigation. Near-infrared imaging could potentially be used to adjust neuronavigation registrations intraoperatively to enhance accuracy. Further prospective studies with distance measurements could better explore this potential benefit of intraoperative near-infrared imaging.

scholarly journals Real-time, Near-Infrared Fluorescence Imaging with an Optimized Dye/Light Source/Camera Combination for Surgical Guidance of Prostate Cancer

2014 ◽  
Vol 21 (4) ◽  
pp. 771-780 ◽  
Author(s):  
Brian P. Neuman ◽  
John B. Eifler ◽  
Mark Castanares ◽  
Wasim H. Chowdhury ◽  
Ying Chen ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Light Source ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Surgical Guidance ◽  
Near Infrared Fluorescence Imaging

Near-infrared ratiometric fluorescence imaging of lysosomal polarity in live cells and in vivo

2021 ◽  
pp. 130397
Author(s):  
Xijuan Chao ◽  
Yan Hu ◽  
Rongjian Liu ◽  
Dejun Huang ◽  
Yingmei Zhang
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Live Cells ◽  
Ratiometric Fluorescence

Robotic Real-time Near Infrared Targeted Fluorescence Imaging in a Murine Model of Prostate Cancer: A Feasibility Study

Urology ◽  
2013 ◽  
Vol 81 (2) ◽  
pp. 451-457 ◽  
Author(s):  
Humberto Laydner ◽  
Steve S. Huang ◽  
Warren D. Heston ◽  
Riccardo Autorino ◽  
Xinning Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Feasibility Study ◽  
Murine Model ◽  
Near Infrared
Sign in / Sign up

Export Citation Format

Share Document