A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity

2018 ◽  
Vol 54 (97) ◽  
pp. 13678-13681 ◽  
Author(s):  
Dandan Zhang ◽  
Fei Ma ◽  
Junhong Leng ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Ultrasensitive Detection ◽  
Argonaute 2 ◽  
Dual Signal Amplification

We develop a new dual-signal amplification-assisted DNAzyme biosensor for sensitive detection of Argonaute 2 (Ago2) activity.

Self-assembled poly-HRP dual signal amplification strategy for high-sensitive detection of circulating miR-142-3p in human serum

2019 ◽  
Vol 279 ◽  
pp. 440-446 ◽  
Author(s):  
Leiliang He ◽  
Lihua Ding ◽  
Songcheng Yu ◽  
Fei Yu ◽  
Yongmei Tian ◽  
...  
Keyword(s):  
Human Serum ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Self Assembled ◽  
Amplification Strategy ◽  
Dual Signal Amplification

scholarly journals Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4364
Author(s):  
Shouping Zhang ◽  
Bin Hu ◽  
Xiaojing Xia ◽  
Yanzhao Xu ◽  
Bolin Hang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Secondary Infection ◽  
Porcine Circovirus Type ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Ability ◽  
Strong Binding ◽  
Dual Signal Amplification

The frequent emergence of secondary infection and immunosuppression after porcine circovirus type 2 (PCV2) infection highlights the need to develop sensitive detection methods. A dual-signal amplification enzyme-linked immunosorbent assay (ELISA) based on a microplate coated with gold nanoparticle layers (GNPL) and tyramide signal amplification (TSA) was established. Results confirmed that the microplates coated with GNPL have a strong binding ability to the antibody without affecting the biological activity of the antibody. The microplates coated with GNPL have strong binding ability to the antibody, and the amplification of the tyramide signal is combined to further improve the sensitivity of PCV2. The PCV2 antibody does not crossreact with other viruses, demonstrating that the method has good specificity. A dual-signal amplification strategy is developed using microplates modified with GNPL and TSA to sensitively detect PCV2.

Gold Nanoparticles and Cleavage-Based Dual Signal Amplification for Ultrasensitive Detection of Silver Ions

2013 ◽  
Vol 85 (16) ◽  
pp. 7966-7970 ◽  
Author(s):  
Peng Miao ◽  
Limin Ning ◽  
Xiaoxi Li
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Silver Ions ◽  
Ultrasensitive Detection ◽  
Dual Signal Amplification

Integration of a peptide–DNA conjugate with multiple cyclic signal amplification for the ultrasensitive detection of cathepsin B activity

2020 ◽  
Vol 56 (14) ◽  
pp. 2119-2122 ◽  
Author(s):  
Zi-yue Wang ◽  
Cheng-peng Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Cathepsin B ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Ultrasensitive Detection

We integrate a peptide–DNA conjugate with multiple cyclic signal amplification for the sensitive detection of cathepsin B activity.

Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification

2019 ◽  
Vol 55 (48) ◽  
pp. 6827-6830 ◽  
Author(s):  
Yan Zhang ◽  
Xin-yan Wang ◽  
Xianwei Su ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
New Method ◽  
Sensitive Detection ◽  
Ultrasensitive Detection ◽  
Non Coding Rnas

We develop a new method for sensitive detection of long noncoding RNAs using duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification.

scholarly journals Dual signal amplification by polysaccharide and eATRP for ultrasensitive detection of CYFRA 21–1 DNA

2020 ◽  
Vol 150 ◽  
pp. 111895 ◽  
Author(s):  
Liying Zhao ◽  
Huaixia Yang ◽  
Xiaoke Zheng ◽  
Jinge Li ◽  
Lihe Jian ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Dual Signal Amplification

Ultrasensitive detection of staphylococcal enterotoxin B in foodstuff through dual signal amplification by bio-barcode and real-time PCR

2019 ◽  
Vol 283 ◽  
pp. 338-344 ◽  
Author(s):  
Yanyang Xu ◽  
Bingyang Huo ◽  
Chao Li ◽  
Yuan Peng ◽  
Sai Tian ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Signal Amplification ◽  
Staphylococcal Enterotoxin ◽  
Ultrasensitive Detection ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B ◽  
Dual Signal Amplification
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