Conductive silk–polypyrrole composite scaffolds with bioinspired nanotopographic cues for cardiac tissue engineering

Imparting electroconductive and nanotopographical cues to biodegradable silk–fibroin films enhanced the maturation of cultured human stem cell-derived cardiomyocytes.

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Cardiac tissue engineering using human stem cell-derived cardiomyocytes for disease modeling and drug discovery

Drug Discovery Today Disease Models ◽

10.1016/j.ddmod.2012.11.001 ◽

2012 ◽

Vol 9 (4) ◽

pp. e219-e227 ◽

Cited By ~ 3

Author(s):

Irene C. Turnbull ◽

Deborah K. Lieu ◽

Ronald A. Li ◽

Kevin D. Costa

Keyword(s):

Tissue Engineering ◽

Stem Cell ◽

Drug Discovery ◽

Cardiac Tissue ◽

Disease Modeling ◽

Cardiac Tissue Engineering ◽

Human Stem Cell

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Application potential of three-dimensional silk fibroin scaffold using mesenchymal stem cells for cardiac regeneration

Journal of Biomaterials Applications ◽

10.1177/08853282211018529 ◽

2021 ◽

pp. 088532822110185

Author(s):

Yuksel Cetin ◽

Merve G Sahin ◽

Fatma N Kok

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Silk Fibroin ◽

Cardiac Tissue ◽

Troponin I ◽

Cardiac Tissue Engineering ◽

Swelling Ratio ◽

Differentiation Potential ◽

Cardiomyogenic Differentiation

Cardiac tissue engineering focusing on biomaterial scaffolds incorporating cells from different sources has been explored to regenerate or repair damaged area as a lifesaving approach.The aim of this study was to evaluate the cardiomyocyte differentiation potential of human adipose mesenchymal stem cells (hAD-MSCs) as an alternative cell source on silk fibroin (SF) scaffolds for cardiac tissue engineering. The change in surface morphology of SF scaffolds depending on SF concentration (1–6%, w/v) and increase in their porosity upon application of unidirectional freezing were visualized by scanning electron microscopy (SEM). Swelling ratio was found to increase 2.4 fold when SF amount was decreased from 4% to 2%. To avoid excessive swelling, 4% SF scaffold with swelling ratio of 10% (w/w) was chosen for further studies.Biodegradation rate of SF scaffolds depended on enzymatic activity was found to be 75% weight loss of SF scaffolds at the day 14. The phenotype of hAD-MSCs and their multi-linage potential into chondrocytes, osteocytes, and adipocytes were shown by flow cytometry and immunohistochemical staining, respectively.The viability of hAD-MSCs on 3D SF scaffolds was determined as 90%, 118%, and 138% after 1, 7, and 14 days, respectively. The use of 3D SF scaffolds was associated with increased production of cardiomyogenic biomarkers: α-actinin, troponin I, connexin 43, and myosin heavy chain. The fabricated 3D SF scaffolds were proved to sustain hAD-MSCs proliferation and cardiomyogenic differentiation therefore, hAD-MSCs on 3D SF scaffolds may useful tool to regenerate or repair damaged area using cardiac tissue engineering techniques.

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Fiber alignment and coculture with fibroblasts improves the differentiated phenotype of murine embryonic stem cell-derived cardiomyocytes for cardiac tissue engineering

Biotechnology and Bioengineering ◽

10.1002/bit.23353 ◽

2011 ◽

Vol 109 (3) ◽

pp. 813-822 ◽

Cited By ~ 76

Author(s):

Ian C. Parrag ◽

Peter W. Zandstra ◽

Kimberly A. Woodhouse

Keyword(s):

Tissue Engineering ◽

Stem Cell ◽

Embryonic Stem Cell ◽

Cardiac Tissue ◽

Embryonic Stem ◽

Cardiac Tissue Engineering ◽

Fiber Alignment ◽

Murine Embryonic Stem Cell

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Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

Polymers for Advanced Technologies ◽

10.1002/pat.4765 ◽

2019 ◽

Vol 31 (2) ◽

pp. 248-259 ◽

Cited By ~ 4

Author(s):

Hojjatollah Nazari ◽

Asieh Heirani‐Tabasi ◽

Maryam Hajiabbas ◽

Masoud Khalili ◽

Mohammadhossein Shahsavari Alavijeh ◽

...

Keyword(s):

Tissue Engineering ◽

Silk Fibroin ◽

Cardiac Tissue ◽

Cardiac Tissue Engineering ◽

Two Dimensional ◽

Silk Fibroin Nanofibers

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Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering

Biomaterials Science ◽

10.1039/c9bm00434c ◽

2019 ◽

Vol 7 (9) ◽

pp. 3906-3917 ◽

Cited By ~ 16

Author(s):

Kaveh Roshanbinfar ◽

Zahra Mohammadi ◽

Abdorreza Sheikh-Mahdi Mesgar ◽

Mohammad Mehdi Dehghan ◽

Oommen P. Oommen ◽

...

Keyword(s):

Carbon Nanotubes ◽

Tissue Engineering ◽

Stem Cell ◽

Carbon Nanotube ◽

Cardiac Tissue ◽

Cardiac Tissue Engineering ◽

Promising Material ◽

Positively Charged

Biohybrid hydrogels consisting of solubilized nanostructured pericardial matrix and electroconductive positively charged hydrazide-conjugated carbon nanotubes provide a promising material for stem cell-based cardiac tissue engineering.

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