An aggregation-induced phosphorescence probe for calcium ion-specific detection and live-cell imaging in Arabidopsis thaliana

2019 ◽  
Vol 55 (33) ◽  
pp. 4841-4844 ◽  
Author(s):  
Guilin Chen ◽  
Zaicai Zhou ◽  
Hui Feng ◽  
Chenyan Zhang ◽  
Yifan Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Calcium Ion ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Molecular Probe ◽  
Specific Detection ◽  
Phosphorescence Probe

A molecular probe with aggregation-induced phosphorescence (AIP) properties for calcium ion-specific detection and imaging in vivo was designed.

A new mitochondrion targetable fluorescent probe for carbon monoxide-specific detection and live cell imaging

2019 ◽  
Vol 55 (64) ◽  
pp. 9444-9447 ◽  
Author(s):  
Chenxia Zhang ◽  
Hai Xie ◽  
Tongxia Zhan ◽  
Jie Zhang ◽  
Bochao Chen ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Biological Systems ◽  
Live Cell ◽  
Molecular Probe ◽  
Powerful Method ◽  
Specific Detection

A red molecular probe for carbon monoxide (CO)-specific detection based on palladium-free mediated opening of spirolactam has been applied to establish a safe and powerful method to detect and image CO changes in biological systems.

A new visible-light-excitable ICT-CHEF-mediated fluorescence ‘turn-on’ probe for the selective detection of Cd2+ in a mixed aqueous system with live-cell imaging

2015 ◽  
Vol 44 (12) ◽  
pp. 5763-5770 ◽  
Author(s):  
Shyamaprosad Goswami ◽  
Krishnendu Aich ◽  
Sangita Das ◽  
Chitrangada Das Mukhopadhyay ◽  
Deblina Sarkar ◽  
...  
Keyword(s):  
Visible Light ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous System ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A new quinoline based sensor was developed and applied for the selective detection of Cd2+ both in vitro and in vivo.

Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

2017 ◽  
pp. 47-54 ◽  
Author(s):  
Daichi Susaki ◽  
Daisuke Maruyama ◽  
Ramesh Yelagandula ◽  
Frederic Berger ◽  
Tomokazu Kawashima
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Actin Dynamics

scholarly journals Regeneration of the neurogliovascular unit visualized in vivo by transcranial live-cell imaging

2020 ◽  
Vol 343 ◽  
pp. 108808 ◽  
Author(s):  
Margarita Arango-Lievano ◽  
Yann Dromard ◽  
Pierre Fontanaud ◽  
Chrystel Lafont ◽  
Patrice Mollard ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals A NIR-emitting cyanine with large Stokes shifts for live cell imaging: large impact of the phenol group on emission

2019 ◽  
Vol 55 (88) ◽  
pp. 13223-13226 ◽  
Author(s):  
Dipendra Dahal ◽  
Lucas McDonald ◽  
Sabita Pokhrel ◽  
Sailaja Paruchuri ◽  
Michael Konopka ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Molecular Probe ◽  
Large Impact ◽  
Phenol Group ◽  
Stokes Shifts ◽  
Excellent Selectivity

A near-infrared (NIR) emitting (λem = 700–900 nm) molecular probe exhibits excellent selectivity towards mitochondria for live-cell imaging.

scholarly journals Detection and Characterization of Protein Interactions In Vivo by a Simple Live-Cell Imaging Method

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62195 ◽  
Author(s):  
Oriol Gallego ◽  
Tanja Specht ◽  
Thorsten Brach ◽  
Arun Kumar ◽  
Anne-Claude Gavin ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Method

scholarly journals Live Cell Imaging of Butterfly Pupal and Larval Wings In Vivo

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128332 ◽  
Author(s):  
Yoshikazu Ohno ◽  
Joji M. Otaki
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals PinMol: Python application for designing molecular beacons for live cell imaging of endogenous mRNAs

2018 ◽  
Author(s):  
Livia V. Bayer ◽  
Omar S. Omar ◽  
Diana P. Bratu ◽  
Irina E. Catrina
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Molecular Beacon ◽  
Structural Information ◽  
Live Cell ◽  
Molecular Beacons ◽  
Intramolecular Interactions ◽  
Live Cells ◽  
Target Rna

ABSTRACTMolecular beacons are nucleic acid oligomers labeled with a fluorophore and a quencher that fold in a hairpin-shaped structure, which fluoresce only when bound to their target RNA. They are used for the visualization of endogenous mRNAs in live cells. Here, we report a Python program (PinMol) that designs molecular beacons best suited for live cell imaging by using structural information from secondary structures of the target RNA, predicted via energy minimization approaches. PinMol takes into account the accessibility of the targeted regions, as well as the inter- and intramolecular interactions of each selected probe. To demonstrate its applicability, we synthesized an oskar mRNA-specific molecular beacon (osk1236), which is selected by PinMol to target a more accessible region than a manually designed oskar-specific molecular beacon (osk2216). We previously demonstrated osk2216 to be efficient in detecting oskar mRNA in in vivo experiments. Here, we show that osk1236 outperformed osk2216 in live cell imaging experiments.

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